Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,860	T→C	L490L (CTT→CTC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,860	0	T	C	100.0%	259.3 / NA	82	L490L (CTT→CTC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/48);  total (34/48)

GTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCT  >  NC_000913/23613‑24106                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         gTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGaa                                                                                                                                                                                                                                                       <  1:413633/251‑1 (MQ=255)    acaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTAGATGGTTGCTAACCGTCCTGCC‑TGGTGTATCTCCCGTCAGCGAACCTGGGGCGTACCGATGTCCCTGATCGGGCCCAAAG‑ACACGGAAAAACTGCAGCCGAGTCCCCTCGAACTg                                                                                                                                                                                                                                                    >  2:453298/1‑251 (MQ=255)     cgtaaCGCCGTGCATCTGCCGCGGGTCGCCGCAGTGGTTCGTGTGTATGGATCGGAAAGGGTGGCGTGCGCATTCATTGTATGAGATCAAAGGCGTGCCGTGGTTCCCGGACTGGTGCCAGGGGCGTATCGAGTCGAGGGTTGCTCACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCTCCTGGGGCGTACCGATGTCCCTGGTCTTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTATCCTCGAACTGa                                                                                                                                                                                                                                                   <  2:265810/248‑1 (MQ=255)      aaaaCGCCGATCATCTGCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGAt                                                                                                                                                                                                                                                  <  1:321888/251‑1 (MQ=255)        aaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCACAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGCGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCCCCTGGGGCGTACCGCTGTCACTGTTCGGGCACAACG‑ACTCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATgg                                                                                                                                                                                                                                                >  2:191976/1‑251 (MQ=255)        aaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATgg                                                                                                                                                                                                                                                >  1:158392/1‑251 (MQ=255)         aCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCCGAAAGGTCTGCGTGCGCAGGCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGGCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGga                                                                                                                                                                                                                                               <  2:448336/251‑1 (MQ=255)         aCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATgg                                                                                                                                                                                                                                                >  1:83428/1‑250 (MQ=255)            ccGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGga                                                                                                                                                                                                                                               >  1:391510/1‑248 (MQ=255)                       ccGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAAc                                                                                                                                                                                                                                 <  2:116662/251‑1 (MQ=255)                               cgccgcAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGaa                                                                                                                                                                                                                         <  1:475728/251‑1 (MQ=255)                                gccgcAGTGGTTCGTCAGCATGGATCAAAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTACTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGCGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGa                                                                                                                                                                                                                          >  1:193170/1‑249 (MQ=255)                                     aGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑ttaac                                                                                                                                                                                                                  >  1:358757/1‑247 (MQ=255)                                          ttCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGGTGAAG‑ttaacggcct                                                                                                                                                                                                             >  2:73381/1‑242 (MQ=255)                                             gtcagcgaggaacagaaaggacctcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGGGGATCCCGGACTTGGGCCATGCGCGTATCGAGCCGATGGTTGATAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTTCCGATGTCACGGTTCGTGCACAATG‑ACGCGGAAGAGCTGCATGCGCGTACCCTCGATCTGATGGAAGAAGTTCCAAAACGCGTTGAAG‑TTAAT‑GGCATCCa                                                                                                                                                                                                         <  2:483235/227‑1 (MQ=255)                                               cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAgg                                                                                                                                                                                                       <  1:202902/251‑1 (MQ=255)                                                          agacaGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGAt                                                                                                                                                                                            <  1:395486/247‑1 (MQ=255)                                                                  cTGCGTGCACAGCCACTGAAAGAGCTCGAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTGACTCCCGTCAGCGACCCTGGGGCGTACCGATGTCACTGTGCGTGAACAAAGGACA‑GGAAGAGCAGCATCCGCGTACCCTCATGCCGATGGAAGCCGAGGCAAAACGCGGTGAAGGTC‑AC‑GGCATCCACGCCGGGTGGGAGCTCGATGc                                                                                                                                                                                    >  2:298551/1‑251 (MQ=255)                                                                         ggcAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACATGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAagag                                                                                                                                                                             <  1:23250/250‑1 (MQ=255)                                                                         cgcAGTCACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTAGCGAGGCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAagag                                                                                                                                                                             <  2:125094/251‑1 (MQ=255)                                                                           cAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑ttaacgg                                                                                                                                                                                                                >  1:131114/1‑209 (MQ=255)                                                                              tCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCAGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGGTGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATATCGATGCGAAAGAGATcat                                                                                                                                                                        >  2:409289/1‑249 (MQ=255)                                                                               cACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTc                                                                                                                                                                       <  1:79061/251‑1 (MQ=255)                                                                                  tGAAAGAGGTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCATCCTGAC‑TGGTGTCTCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAGG‑ACACGCACGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGCAGTGGCAACTCCCGTTGAGG‑AGAAC‑GGCATCCAGGCGTGGTGGGaccccggtgagaacgagatcaacggg                                                                                                                                                                    >  2:444009/1‑226 (MQ=255)                                                                                          aTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc                                                                                                                                                            <  1:49613/251‑1 (MQ=255)                                                                                               aGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCTAAAGAGATCCTCGGCGACGAAGCTGacc                                                                                                                                                       <  1:433753/251‑3 (MQ=255)                                                                                                 gCGTGCAGTGGATCCCGGACTGGGGTCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAg                                                                                                                                                     <  1:112495/251‑1 (MQ=255)                                                                                                     gCAGTGGATCCCGGACTGGGGCCAGGCGAGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCTAAATAGATCCTCGGCGACGAAGCTGACCAGTatg                                                                                                                                                 <  1:145560/251‑3 (MQ=255)                                                                                                          ggATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGaaa                                                                                                                                            <  1:355721/251‑1 (MQ=255)                                                                                                            aTCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGACATGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCCGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAACTGACCAGTATGTGTAAgg                                                                                                                                          >  2:370176/1‑250 (MQ=255)                                                                                                               ccGGTCTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTACCCGTCCTGAC‑TGGTGTATCTCCCGTCTGCGCCCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACGCGGAAGAGCTGCATCCGCGTACACGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                                                                                       <  2:128615/251‑1 (MQ=255)                                                                                                               ccGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                                                                                       <  1:274520/251‑1 (MQ=255)                                                                                                                    cgggggCCCGGGGCGTATCGAGTCGATGGTTGCTAACCGGCCTGACATGG‑GTATATCCCGTCAGCGCACCTGGGGCGTACCGATGACACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATACAGGCGTGGTGGGAGCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGata                                                                                                                                  <  2:308383/249‑3 (MQ=255)                                                                                                                     tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatac                                                                                                                                 >  1:85138/1‑248 (MQ=255)                                                                                                                     tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGCCGAAGCTGACCAGTCTGTGAAAGGGCCGGatac                                                                                                                                 >  2:242477/1‑248 (MQ=255)                                                                                                                      ggtgccgggCGCTTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGGCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAATCGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGata                                                                                                                                  <  2:182285/242‑3 (MQ=255)                                                                                                                      ggggcagagggcgTATCGAGTCGAGGGTTGCTAACCGTCCTGAC‑TGTTGTATCTCCCGTCAGCGCTCCGGGGGCGTACCGATGTCACTGGTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCTCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCACCCAGGCGTGGTGGGATCTCGATGCGATAGAGATCCTCGGCGACGAAGCTGCCCATTATGTGAAAGTGCCGGatact                                                                                                                                <  2:419814/241‑5 (MQ=255)                                                                                                                            aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                          <  1:491466/251‑11 (MQ=255)                                                                                                                            aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                          <  2:461034/251‑11 (MQ=255)                                                                                                                                    aTCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtatggtt                                                                                                                  <  1:169492/251‑19 (MQ=255)                                                                                                                                        gggcGATGGTTGCGAACCGGCCTGAC‑TGGGGTAACTCCAGAAATCGCACCAGGGGCGGACCGATGTCACTGTACGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATTCAGTCGTGGTGGGATCTCGATACGAAAGAGAGCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTggc                                                                                                              <  2:249766/248‑3 (MQ=255)                                                                                                                                        aGTCGATGGGTGCTAACCGTCCTGAC‑TGGCGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTAACCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtagggtttgtc                                                                                                              >  2:293340/1‑229 (MQ=255)                                                                                                                                         gTCGATGGTTGCTACCCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAg                                                                                                                                                                                                        >  2:306909/1‑160 (MQ=255)                                                                                                                                         gTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAg                                                                                                                                                                                                        <  1:306909/160‑1 (MQ=255)                                                                                                                                             aTGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCgg                                                                                                         <  2:435776/251‑1 (MQ=255)                                                                                                                                             aTGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCgg                                                                                                         <  1:470153/251‑1 (MQ=255)                                                                                                                                                     tAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGGATGTGAAAGTGCCGGACACTCTCGCCGAATGGTTTGCCTCCGGATCTcccc                                                                                                 >  2:154678/1‑251 (MQ=255)                                                                                                                                                      aaCCGGCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTGCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAGC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACGCTCGACGTATGGTTTGACTCCGGATCTACCCa                                                                                                <  2:411794/251‑1 (MQ=255)                                                                                                                                                       aCCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCAc                                                                                               >  1:238582/1‑251 (MQ=255)                                                                                                                                                                 c‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGCTGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGCGATCCTCGGCGACGAAGCTGACCAGTATGTGACAGTGCCGGATACTCTCGCCGTATGGTTTGACTCCGGATCTACCCCCTCTTCtgttg                                                                                     >  2:369942/1‑251 (MQ=255)                                                                                                                                                                 c‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGCCCAGTATGTGAAAGTGCCGGATCCTCTCGACGTATGGTTTGACTACGGATCTACCCACTCTTCtgttg                                                                                     >  2:420372/1‑251 (MQ=255)                                                                                                                                                                    ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCGTCtgttg                                                                                     <  1:226231/249‑1 (MQ=255)                                                                                                                                                                        tATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACg                                                                               >  1:306020/1‑251 (MQ=255)                                                                                                                                                                           ctcCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGc                                                                            >  1:46217/1‑251 (MQ=255)                                                                                                                                                                             cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGt                                                                          >  1:174440/1‑251 (MQ=255)                                                                                                                                                                                gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                       <  2:83428/251‑1 (MQ=255)                                                                                                                                                                                   aGCGCAACTGGCGCGTACCAGTGTCCCTGTTCGTGCACAAAG‑GCACGGCAGAGCTGGATTCGAGTACCCTCGAACTGAGGGAAGAAGGTGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCGGTTGTTGACGTGCGTCCGGaa                                                                    <  2:118366/251‑1 (MQ=255)                                                                                                                                                                                    gcgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAt                                                                   <  1:355593/251‑1 (MQ=255)                                                                                                                                                                                      gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt                                                                 <  1:231656/251‑1 (MQ=255)                                                                                                                                                                                       ccccTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACAAGGAAGAGCTGCAGCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGAGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTg                                                                <  2:225673/249‑1 (MQ=255)                                                                                                                                                                                       cACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGACGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTACGGAATTTg                                                                >  2:415489/1‑251 (MQ=255)                                                                                                                                                                                         ccTGGGGCGTACCGATGGCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGcc                                                              <  1:360500/251‑1 (MQ=255)                                                                                                                                                                                                cgtacctaggtcactgtgccggCACAAAG‑ACACGGAAGAGCTGCATCCTCGGACCCGCGAACTGATGGAAGAAGTGGCAAAGCGCGTTGAAG‑T‑TATCGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACTAATCTGACCAGTAGGTGAAAGTGCCGGATACTCTCGACGTATGGTTGGATTCCGGATCTTCCCACTTTTCTTTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                       <  2:463813/230‑1 (MQ=255)                                                                                                                                                                                                 gtACCGATGTCACTGCTCGGGCTCAAGG‑ACTCTGTAGTGTGGCATCCGCGTACACCCGAACTGATGGGAGAAGTGGCAAAGCGCGTTGAAG‑TTAAC‑TGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGAGCCTGATACTCTCTTCGTAGGGTGCGACTCCGGAGCTACCCACTCGTCTGTTGGTGACGTGCGTCCGGAATTTGCCggctacgc                                                      <  2:458354/251‑7 (MQ=255)                                                                                                                                                                                                     cGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCg                                                  >  1:119022/1‑251 (MQ=255)                                                                                                                                                                                                         gTCACTGTTAGAGCACAAAG‑AAACGGAAGAGATGCCTCCGCGTACCCTCGACCTGCTGGAAGAAGTGGCAAACCGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGTCGTCTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGCCGTGAGTCCGGAATTTGCCGGTCACGCAGCGGAcc                                              >  2:500538/1‑250 (MQ=255)                                                                                                                                                                                                          tCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACAt                                             >  1:213132/1‑251 (MQ=255)                                                                                                                                                                                                           cACTGTTCGTGCACAAAT‑GCACCGAAGATCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACAt                                             <  1:30699/250‑1 (MQ=255)                                                                                                                                                                                                           cACTGTTCGTGCACAAAG‑ACTCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGAGCTCGATGCGAAAGAGATCCTCGGCGACGAAGTTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATg                                            <  2:177918/251‑1 (MQ=255)                                                                                                                                                                                                               ggtCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCGTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTa                                          <  2:137850/247‑1 (MQ=255)                                                                                                                                                                                                                 tCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTg                                      >  1:289218/1‑251 (MQ=255)                                                                                                                                                                                                                        caAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGCCCGGAATTTGCCGGTAACGCAGCGGACATGTATCTGGAAGGtt                               <  1:441694/251‑1 (MQ=255)                                                                                                                                                                                                                         aaaG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCCTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGCAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAACGGCCATGTATCTGGAAGGTTc                              >  2:131690/1‑251 (MQ=255)                                                                                                                                                                                                                                     agagCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGTAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGTCGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGCATTTGCCGGTCACGCAGCGGCCATGTATCTGGTAGGTTCTGAcccgcacc                   >  2:150548/1‑245 (MQ=255)                                                                                                                                                                                                                                      gagCTGCATCCGCGTACCCTCGAACTGATGTAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCTGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcg                  <  1:23998/251‑1 (MQ=255)                                                                                                                                                                                                                                           gCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggtt             <  1:262738/251‑6 (MQ=255)                                                                                                                                                                                                                                             aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttgg           <  1:385456/251‑8 (MQ=255)                                                                                                                                                                                                                                                    tACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCCGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATgg    >  2:11691/1‑250 (MQ=255)                                                                                                                                                                                                                                                     aCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt    <  1:371679/250‑1 (MQ=255)                                                                                                                                                                                                                                                     aCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTc   >  1:7771/1‑251 (MQ=255)                                                                                                                                                                                                                                                      cccTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTc   <  1:502111/250‑1 (MQ=255)                                                                                                                                                                                                                                                      cccTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCt  >  1:366720/1‑251 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         GTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCT  >  NC_000913/23613‑24106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑