Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,803	T→C	G471G (GGT→GGC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,803	0	T	C	100.0%	262.0 / NA	87	G471G (GGT→GGC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/53);  total (34/53)

AAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCC  >  NC_000913/23556‑24046                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      aaaaaGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTAAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGgtgt                                                                                                                                                                                                                                                     >  1:88556/1‑250 (MQ=255)   aaaGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTAcc                                                                                                                                                                                                                                                  <  1:362595/251‑1 (MQ=255)   aaaGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCACGTCAGCGCACCTGGGGCGTAcc                                                                                                                                                                                                                                                  >  2:427841/1‑251 (MQ=255)   aaaGGCGCGCGGCTGCACGTGGAGAAAATGCAGCACAGCTACCCGTGCTGCTGGCGGCACAAAACGCCGATCATCTGTCGCGCGTCGCCGCGGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGACGATGGTTGCTAACCGTCCTGAC‑TTGTGTATCTCCCGTCAGCGCGCCTGTGGCGTAcc                                                                                                                                                                                                                                                  <  2:365430/251‑1 (MQ=255)      ggCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGAt                                                                                                                                                                                                                                               <  2:121240/251‑1 (MQ=255)        cgcgcTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGt                                                                                                                                                                                                                                             >  1:56897/1‑251 (MQ=255)              gctgcACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACt                                                                                                                                                                                                                                         <  2:210846/249‑1 (MQ=255)              gctgcACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTg                                                                                                                                                                                                                                        <  2:376895/250‑1 (MQ=255)                 gcACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTc                                                                                                                                                                                                                                     <  1:331891/250‑1 (MQ=255)                 gcACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTc                                                                                                                                                                                                                                     <  1:30555/250‑1 (MQ=255)                     gTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGGTCATCTTCCGCGCGACGCCGCAGTGGATCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTACCCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCCCCTGGGGCGTACCGATGTCACTGTTCGTGc                                                                                                                                                                                                                                 >  1:220371/1‑250 (MQ=255)                       ggagaAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGcaca                                                                                                                                                                                                                              <  2:174334/250‑1 (MQ=255)                          gaAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAg                                                                                                                                                                                                                           <  1:488274/251‑1 (MQ=255)                           aaaaTGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑a                                                                                                                                                                                                                         <  1:82533/251‑1 (MQ=255)                            aaaTGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ac                                                                                                                                                                                                                        >  1:506656/1‑251 (MQ=255)                              aTGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGGACCGATGTCACTGTTCGTGCACAAAA‑acac                                                                                                                                                                                                                      >  2:426267/1‑251 (MQ=255)                                        gCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTGCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTg                                                                                                                                                                                                            <  1:222920/251‑1 (MQ=255)                                         cTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTTTCGAGTCGATGGTGGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATTTCACTGTTCGTGCACAAAG‑ACACgggagggatgc                                                                                                                                                                                                           >  2:405037/1‑242 (MQ=255)                                           aTCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCAt                                                                                                                                                                                                         <  1:357975/251‑1 (MQ=255)                                              cgagctgcgGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCGGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCCGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGGATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACCAAG‑ACAAGGAAGAGCTGCATCcg                                                                                                                                                                                                      >  2:355593/4‑251 (MQ=255)                                              cGTGCTGCTGGCGTAACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCcg                                                                                                                                                                                                      <  2:6885/251‑1 (MQ=255)                                                tgctgcTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCcgcg                                                                                                                                                                                                    >  1:356948/1‑251 (MQ=255)                                                 gctgctGGCGTCACAAAACGCCGATCATCTGCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGTGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCcgcg                                                                                                                                                                                                    <  1:161800/250‑1 (MQ=255)                                                    gctgGCGACACAACACGCCTATCATCCTCCGCGCGACGACGCAGTGGTTCGTCAGCATGGAACAGAAAGGTCTGCGGGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTAc                                                                                                                                                                                                 <  2:380472/250‑1 (MQ=255)                                                     gtgGCGTCACAAAACGCCGATCGTCTTCCGCGCGAGGGCGCAGGGGTTCGTGAGCGTGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTAccc                                                                                                                                                                                               <  1:33365/250‑1 (MQ=255)                                                     ctgGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTAccc                                                                                                                                                                                               >  1:116662/1‑251 (MQ=255)                                                          gTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGaa                                                                                                                                                                                          <  1:413633/251‑1 (MQ=255)                                                             acaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTAGATGGTTGCTAACCGTCCTGCC‑TGGTGTATCTCCCGTCAGCGAACCTGGGGCGTACCGATGTCCCTGATCGGGCCCAAAG‑ACACGGAAAAACTGCAGCCGAGTCCCCTCGAACTg                                                                                                                                                                                       >  2:453298/1‑251 (MQ=255)                                                              cgtaaCGCCGTGCATCTGCCGCGGGTCGCCGCAGTGGTTCGTGTGTATGGATCGGAAAGGGTGGCGTGCGCATTCATTGTATGAGATCAAAGGCGTGCCGTGGTTCCCGGACTGGTGCCAGGGGCGTATCGAGTCGAGGGTTGCTCACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCTCCTGGGGCGTACCGATGTCCCTGGTCTTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTATCCTCGAACTGa                                                                                                                                                                                      <  2:265810/248‑1 (MQ=255)                                                               aaaaCGCCGATCATCTGCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGAt                                                                                                                                                                                     <  1:321888/251‑1 (MQ=255)                                                                 aaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCACAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGCGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCCCCTGGGGCGTACCGCTGTCACTGTTCGGGCACAACG‑ACTCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATgg                                                                                                                                                                                   >  2:191976/1‑251 (MQ=255)                                                                 aaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATgg                                                                                                                                                                                   >  1:158392/1‑251 (MQ=255)                                                                  aCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCCGAAAGGTCTGCGTGCGCAGGCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGGCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGga                                                                                                                                                                                  <  2:448336/251‑1 (MQ=255)                                                                  aCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATgg                                                                                                                                                                                   >  1:83428/1‑250 (MQ=255)                                                                     ccGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGga                                                                                                                                                                                  >  1:391510/1‑248 (MQ=255)                                                                                ccGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAAc                                                                                                                                                                    <  2:116662/251‑1 (MQ=255)                                                                                        cgccgcAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGaa                                                                                                                                                            <  1:475728/251‑1 (MQ=255)                                                                                         gccgcAGTGGTTCGTCAGCATGGATCAAAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTACTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGCGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGa                                                                                                                                                             >  1:193170/1‑249 (MQ=255)                                                                                              aGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑ttaac                                                                                                                                                     >  1:358757/1‑247 (MQ=255)                                                                                                   ttCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGGTGAAG‑ttaacggcct                                                                                                                                                >  2:73381/1‑242 (MQ=255)                                                                                                      gtcagcgaggaacagaaaggacctcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGGGGATCCCGGACTTGGGCCATGCGCGTATCGAGCCGATGGTTGATAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTTCCGATGTCACGGTTCGTGCACAATG‑ACGCGGAAGAGCTGCATGCGCGTACCCTCGATCTGATGGAAGAAGTTCCAAAACGCGTTGAAG‑TTAATGGCATCCa                                                                                                                                             <  2:483235/227‑1 (MQ=255)                                                                                                        cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAgg                                                                                                                                           <  1:202902/251‑1 (MQ=255)                                                                                                                   agacaGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGAt                                                                                                                                <  1:395486/247‑1 (MQ=255)                                                                                                                           cTGCGTGCACAGCCACTGAAAGAGCTCGAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTGACTCCCGTCAGCGACCCTGGGGCGTACCGATGTCACTGTGCGTGAACAAAGGACA‑GGAAGAGCAGCATCCGCGTACCCTCATGCCGATGGAAGCCGAGGCAAAACGCGGTGAAGGTC‑ACGGCATCCACGCCGGGTGGGAGCTCGATGc                                                                                                                        >  2:298551/1‑251 (MQ=255)                                                                                                                                  ggcAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACATGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAagag                                                                                                                 <  1:23250/250‑1 (MQ=255)                                                                                                                                  cgcAGTCACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTAGCGAGGCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAagag                                                                                                                 <  2:125094/251‑1 (MQ=255)                                                                                                                                    cAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑ttaacgg                                                                                                                                                   >  1:131114/1‑209 (MQ=255)                                                                                                                                       tCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCAGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGGTGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATATCGATGCGAAAGAGATcat                                                                                                            >  2:409289/1‑249 (MQ=255)                                                                                                                                        cACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTc                                                                                                           <  1:79061/251‑1 (MQ=255)                                                                                                                                           tGAAAGAGGTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCATCCTGAC‑TGGTGTCTCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAGG‑ACACGCACGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGCAGTGGCAACTCCCGTTGAGG‑AGAACGGCATCCAGGCGTGGTGGGaccccggtgagaacgagatcaacggg                                                                                                        >  2:444009/1‑226 (MQ=255)                                                                                                                                                   aTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc                                                                                                <  1:49613/251‑1 (MQ=255)                                                                                                                                                        aGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCTAAAGAGATCCTCGGCGACGAAGCTGacc                                                                                           <  1:433753/251‑3 (MQ=255)                                                                                                                                                          gCGTGCAGTGGATCCCGGACTGGGGTCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAg                                                                                         <  1:112495/251‑1 (MQ=255)                                                                                                                                                              gCAGTGGATCCCGGACTGGGGCCAGGCGAGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCTAAATAGATCCTCGGCGACGAAGCTGACCAGTatg                                                                                     <  1:145560/251‑3 (MQ=255)                                                                                                                                                                   ggATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGaaa                                                                                <  1:355721/251‑1 (MQ=255)                                                                                                                                                                     aTCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGACATGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCCGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAACTGACCAGTATGTGTAAgg                                                                              >  2:370176/1‑250 (MQ=255)                                                                                                                                                                        ccGGTCTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTACCCGTCCTGAC‑TGGTGTATCTCCCGTCTGCGCCCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACGCGGAAGAGCTGCATCCGCGTACACGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                           <  2:128615/251‑1 (MQ=255)                                                                                                                                                                        ccGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                           <  1:274520/251‑1 (MQ=255)                                                                                                                                                                             cgggggCCCGGGGCGTATCGAGTCGATGGTTGCTAACCGGCCTGACATGG‑GTATATCCCGTCAGCGCACCTGGGGCGTACCGATGACACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATACAGGCGTGGTGGGAGCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGata                                                                      <  2:308383/249‑3 (MQ=255)                                                                                                                                                                              tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatac                                                                     >  1:85138/1‑248 (MQ=255)                                                                                                                                                                              tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGCCGAAGCTGACCAGTCTGTGAAAGGGCCGGatac                                                                     >  2:242477/1‑248 (MQ=255)                                                                                                                                                                               ggtgccgggCGCTTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGGCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAATCGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGata                                                                      <  2:182285/242‑3 (MQ=255)                                                                                                                                                                               ggggcagagggcgTATCGAGTCGAGGGTTGCTAACCGTCCTGAC‑TGTTGTATCTCCCGTCAGCGCTCCGGGGGCGTACCGATGTCACTGGTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCTCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCACCCAGGCGTGGTGGGATCTCGATGCGATAGAGATCCTCGGCGACGAAGCTGCCCATTATGTGAAAGTGCCGGatact                                                                    <  2:419814/241‑5 (MQ=255)                                                                                                                                                                                     aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                              <  1:491466/251‑11 (MQ=255)                                                                                                                                                                                     aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                              <  2:461034/251‑11 (MQ=255)                                                                                                                                                                                             aTCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtatggtt                                                      <  1:169492/251‑19 (MQ=255)                                                                                                                                                                                                 gggcGATGGTTGCGAACCGGCCTGAC‑TGGGGTAACTCCAGAAATCGCACCAGGGGCGGACCGATGTCACTGTACGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATTCAGTCGTGGTGGGATCTCGATACGAAAGAGAGCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTggc                                                  <  2:249766/248‑3 (MQ=255)                                                                                                                                                                                                 aGTCGATGGGTGCTAACCGTCCTGAC‑TGGCGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTAACCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtagggtttgtc                                                  >  2:293340/1‑229 (MQ=255)                                                                                                                                                                                                  gTCGATGGTTGCTACCCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAg                                                                                                                                            >  2:306909/1‑160 (MQ=255)                                                                                                                                                                                                  gTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAg                                                                                                                                            <  1:306909/160‑1 (MQ=255)                                                                                                                                                                                                      aTGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCgg                                             <  2:435776/251‑1 (MQ=255)                                                                                                                                                                                                      aTGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCgg                                             <  1:470153/251‑1 (MQ=255)                                                                                                                                                                                                              tAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGGATGTGAAAGTGCCGGACACTCTCGCCGAATGGTTTGCCTCCGGATCTcccc                                     >  2:154678/1‑251 (MQ=255)                                                                                                                                                                                                               aaCCGGCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTGCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAGCGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACGCTCGACGTATGGTTTGACTCCGGATCTACCCa                                    <  2:411794/251‑1 (MQ=255)                                                                                                                                                                                                                aCCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCAc                                   >  1:238582/1‑251 (MQ=255)                                                                                                                                                                                                                          c‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGCTGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGCGATCCTCGGCGACGAAGCTGACCAGTATGTGACAGTGCCGGATACTCTCGCCGTATGGTTTGACTCCGGATCTACCCCCTCTTCtgttg                         >  2:369942/1‑251 (MQ=255)                                                                                                                                                                                                                          c‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGCCCAGTATGTGAAAGTGCCGGATCCTCTCGACGTATGGTTTGACTACGGATCTACCCACTCTTCtgttg                         >  2:420372/1‑251 (MQ=255)                                                                                                                                                                                                                             ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCGTCtgttg                         <  1:226231/249‑1 (MQ=255)                                                                                                                                                                                                                                 tATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACg                   >  1:306020/1‑251 (MQ=255)                                                                                                                                                                                                                                    ctcCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGc                >  1:46217/1‑251 (MQ=255)                                                                                                                                                                                                                                      cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGt              >  1:174440/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg           <  2:83428/251‑1 (MQ=255)                                                                                                                                                                                                                                            aGCGCAACTGGCGCGTACCAGTGTCCCTGTTCGTGCACAAAG‑GCACGGCAGAGCTGGATTCGAGTACCCTCGAACTGAGGGAAGAAGGTGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCGGTTGTTGACGTGCGTCCGGaa        <  2:118366/251‑1 (MQ=255)                                                                                                                                                                                                                                             gcgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAt       <  1:355593/251‑1 (MQ=255)                                                                                                                                                                                                                                               gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt     <  1:231656/251‑1 (MQ=255)                                                                                                                                                                                                                                                ccccTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACAAGGAAGAGCTGCAGCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGAGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTg    <  2:225673/249‑1 (MQ=255)                                                                                                                                                                                                                                                cACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGACGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTACGGAATTTg    >  2:415489/1‑251 (MQ=255)                                                                                                                                                                                                                                                  ccTGGGGCGTACCGATGGCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGcc  <  1:360500/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      AAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCC  >  NC_000913/23556‑24046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑