Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,108	A→T	Q591L (CAG→CTG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,108	0	A	T	100.0%	201.6 / NA	65	Q591L (CAG→CTG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (31/34);  total (31/34)

CTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATG  >  NC_000913/1861‑2353                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       ctGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGtt                                                                                                                                                                                                                                                     >  1:326550/1‑251 (MQ=255)   gCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGc                                                                                                                                                                                                                                                   <  1:53559/251‑1 (MQ=255)                       taaaTCTGGAAAACTGACAGGCAGAACTTGCGCAAGCCAAATAGACGTTTAATCTCAGGCGCTTAATTCGCCTCGGGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATAAATATGCCGACTTCTTG‑TGCGAAGGTTTCCCCGATTTCAAGCCGAACACAAAGGCCAACAACTCGTCGCTGGATTTCTACCATCTGTTGCGTAATGAGGCGGaaacaac                                                                                                                                                                                                                                >  2:320511/3‑246 (MQ=255)                              ggAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTa                                                                                                                                                                                                                        <  1:82318/251‑1 (MQ=255)                              ggAAAACGGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCgcggcg                                                                                                                                                                                                                          <  1:85655/249‑1 (MQ=255)                                   acgggCAGGAAGAACTGGCGCAAGCAAAAGAGCCGTTTAATCTCTGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTc                                                                                                                                                                                                                   <  2:429785/248‑1 (MQ=255)                                        cAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAAAACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCctctt                                                                                                                                                                                                              >  1:70853/1‑250 (MQ=255)                                                  gggcccAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAAATCGCCGCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTTGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGGCGATGGATTACTACCATCTGGTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAAc                                                                                                                                                                                                   <  2:48300/246‑1 (MQ=255)                                                        aaGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGCAGGTTTCCCCGTTGGCACGCCGAACAAAAAAGCCAACACCTCGTCGATGGATTACTACCATCTGTTGAGTCATGCGGCGGAAAAATCGCGGGGCAAATTCCTCCATTAC‑ACCCACGTTggg                                                                                                                                                                                             >  2:347033/1‑251 (MQ=255)                                                           ccAAAGAGCCGTTTAATCTCGGGCGCTTACTTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGAATGCACTTCCAGCCAGGCAGTGGCGGATTAACATGCCAACTTCTTG‑AGCGGAGGTTTCCACGTTGTCAAGACGCAAAAAAAGGACAACACCTCGACGCTGGATTCATACCATCTGTTGCGTCATGCGGAGGAAAACTCGCGGCGTAAATTCCTCTATGAC‑AACAACGTTGGGGCt                                                                                                                                                                                          >  1:24689/1‑251 (MQ=255)                                                            cAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTg                                                                                                                                                                                         >  1:473497/1‑251 (MQ=255)                                                             aaatagCCGTTTAATCTCGGGCGCGTAAGTCGCCTCGTGGAAGAAGATCATCGGCTGAGACCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTGCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGGCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTgg                                                                                                                                                                                        <  2:114108/247‑1 (MQ=255)                                                              aaGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGGTGTCACCCCGAACAAAAAGGCCAACTCCTCGTCGATGAGTAACTACCCCCTGTTGCGTCCTTCGGCGGAAAAATCGCGGCGGAAATTCCTCTCTTAC‑ACCATCGTTgggggtagt                                                                                                                                                                                       >  1:147889/1‑246 (MQ=255)                                                                     gTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGTAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCgg                                                                                                                                                                                <  1:232701/251‑1 (MQ=255)                                                                      tttAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGt                                                                                                                                                                               >  1:339846/1‑251 (MQ=255)                                                                              agggCGCTTAATCTGCCTCGTGAAAGAATATCATCGGCTGAACCCGGTCATTGTTGACTTCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑CCCAACGTTGGGGCTGGATTACCGGTTATTga                                                                                                                                                                         <  2:37748/248‑1 (MQ=255)                                                                                  cgcTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTTCCGGTTATTGAGAACCt                                                                                                                                                                   >  1:492039/1‑251 (MQ=255)                                                                                   gcTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTg                                                                                                                                                                  <  2:403446/251‑1 (MQ=255)                                                                                    cTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGGAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGc                                                                                                                                                                 >  1:73613/1‑251 (MQ=255)                                                                                           cGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATc                                                                                                                                                           <  1:3115/250‑1 (MQ=255)                                                                                                cGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCGCTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCa                                                                                                                                                     <  1:87469/251‑1 (MQ=255)                                                                                                 gTGAAAGAATATCCTCTGCTGAACCCGGCCATTGTTGACTGCACTTCCAGCCAGGCGGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCa                                                                                                                                                     >  1:412795/1‑250 (MQ=255)                                                                                                    aaaGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAAcc                                                                                                                                                                    <  2:274471/232‑1 (MQ=255)                                                                                                    aaaGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAAcc                                                                                                                                                                    >  1:274471/1‑232 (MQ=255)                                                                                                    aaaGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGc                                                                                                                                                 >  1:90775/1‑251 (MQ=255)                                                                                                     aaGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATTTGCCGACTTCTTT‑CGCGAAGGGTTCCACGTTGTCACGCCGACCAAAAAGGCCCACACCTCGGCGATGGATTAATACCATCTGTTGCGCCCAGAGGCGGAAAAAACGCGGCGGACATTCCTCTATTAC‑CACACAGTTGGGGCTTGATTACCGGTTATTGAGAACCTTCAAAAACTGCTTAATGct                                                                                                                                                >  2:187308/1‑250 (MQ=255)                                                                                                       gAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCaaagctgg                                                                                                                                              >  1:28912/1‑245 (MQ=255)                                                                                                       gAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGACAACACCTCGTCGATGGATTACTACCATCTGGTGCGTCATGCGGCGGAAAAATCGCGGCGTAAAATCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGATCATTCAAAATCTGCTCAATgatgg                                                                                                                                              >  1:12012/1‑247 (MQ=255)                                                                                                             catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCTCGGCGTAAATTCCTCTATGAC‑ACCCACGTTGGGGCTGGCTTACCGGTTATTGGGAACCTGCAAAACTTGCTCAATGcttggtgtg                                                                                                                                         >  2:377939/1‑242 (MQ=255)                                                                                                             catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtgatg                                                                                                                                         <  1:136969/250‑1 (MQ=255)                                                                                                                        cccGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGt                                                                                                                             <  1:61987/251‑1 (MQ=255)                                                                                                                               aTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCgg                                                                                                                      <  2:90775/251‑1 (MQ=255)                                                                                                                                     gACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttct                                                                                                                <  1:377939/251‑1 (MQ=255)                                                                                                                                         gCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGAGTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctttca                                                                                                            <  2:278264/251‑2 (MQ=255)                                                                                                                                          cACTTCCAGCCAGGCAGTGGGGGATCAATATGCCGACTTCTTG‑CGCGAAGGGTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctttcag                                                                                                           <  2:73613/251‑3 (MQ=255)                                                                                                                                           aCTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGCAAAATCGCGGCGTAAATTCCTCTATGACCACCA‑CGTTGGGGCTGGATTACCGGATATTGTGAACCTGCAAAATCTGCTAAATGCTGGTGATGAATTGATTTAGTTCTCCGCCAttctttcagg                                                                                                          >  2:394727/1‑248 (MQ=255)                                                                                                                                              tCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGt                                                                                                         <  1:197270/249‑1 (MQ=255)                                                                                                                                                 agccagGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATtgat                                                                                                                                  <  2:176907/221‑1 (MQ=255)                                                                                                                                                 agccagGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGGTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATtgat                                                                                                                                  >  1:176907/1‑221 (MQ=255)                                                                                                                                                   ccaagCAGTGGCGGATCAATATGCCGAATTCGTG‑CGCGAAGGTGTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTGCCATCTGTTGCGGCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTAGGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCCCAATGCTTGGGATGAATTGATGAAGTTCTCCGTCATTCTTTCTGGCTCGCttt                                                                                                  <  2:329950/247‑1 (MQ=255)                                                                                                                                                          gTGGCGGTGCAATATGCCGTCTTCTTGACG‑GAAGGTTTCCCAGTTGTCACGCCTAACAAAAAGGCCAGCACCTCGGCGATGGAGTACTACCATCTTTTGCTTCATGCTGCGGAAAAGTCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGTGGCTGTATTACCGGTTATGTAGATCCTGCAAATTCTGCTCTATGCTTGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGCCTCGCTTTCTTatat                                                                                           <  2:444020/251‑1 (MQ=255)                                                                                                                                                            ggCGGATCAATAGTCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATGCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATc                                                                                          <  2:422611/250‑1 (MQ=255)                                                                                                                                                             gCGGAACAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCtt                                                                                        <  2:326550/251‑1 (MQ=255)                                                                                                                                                                     atatGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGCATTGCTGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGt                                                                                >  2:182059/1‑251 (MQ=255)                                                                                                                                                                         gCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAAGCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGa                                                                            <  1:300740/251‑1 (MQ=255)                                                                                                                                                                         gCCGACTGCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGa                                                                            <  1:248510/251‑1 (MQ=255)                                                                                                                                                                            gACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg                                                                          >  1:404375/1‑250 (MQ=255)                                                                                                                                                                                   tG‑CGCGAAGGTTTCAACGTTGTCACGCCGAACAAAAAGGACAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATACCTCTATGAC‑ACCAACGTTGGGACTAGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCAt                                                                   <  2:473497/250‑1 (MQ=255)                                                                                                                                                                                         gAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGTTGAAGTTCTCCGGCTTTCTTTCAGGTTCGCTTTCTTATATCTTCGGCATGTTAGACGAAGGCTTGAGttt                                                             >  2:423138/1‑251 (MQ=255)                                                                                                                                                                                         gAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGttt                                                             >  2:223725/1‑251 (MQ=255)                                                                                                                                                                                          aaGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTACGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTtc                                                            >  1:328600/1‑251 (MQ=255)                                                                                                                                                                                                tCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTTTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCg                                                        >  2:238844/1‑249 (MQ=255)                                                                                                                                                                                                        gTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTTGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGGTAGACGAAGGCATGAGTTTCTCCGAGGCGActa                                               <  1:438963/250‑3 (MQ=255)                                                                                                                                                                                                           aCGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATCTCTTCGGCAAGTTAGACGAAGGCATGAGTTTATCCGAGGCGActactct                                           >  2:246558/1‑245 (MQ=255)                                                                                                                                                                                                            cGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGActactctg                                          <  2:404375/251‑8 (MQ=255)                                                                                                                                                                                                             cccGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCAGGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGAGGAAGTTCTTCGGCATTCTTTCAGGTTCGCTTTCGTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTGCTCCGAGGCGACTACTCTgg                                         <  2:499677/250‑1 (MQ=255)                                                                                                                                                                                                                 aaCAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGcgcg                                     >  2:291079/1‑251 (MQ=255)                                                                                                                                                                                                                   cAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACAGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGGAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCggg                                   >  2:59206/1‑251 (MQ=255)                                                                                                                                                                                                                     aaaaGTCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCATGTTCGCTTTCTTATATCTTCGGCa                                                                                   <  1:279767/201‑1 (MQ=255)                                                                                                                                                                                                                     aaaaGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCa                                                                                   >  2:279767/1‑201 (MQ=255)                                                                                                                                                                                                                         ggccacagccTCGTCGATGGAGTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATTAC‑ACCATCGTGGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATTTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCGTTTAGGTTCGCTTTCTTATATCTTCGGTAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATgg                             <  1:300402/243‑1 (MQ=255)                                                                                                                                                                                                                             aaCACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAACCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTta                         <  1:441224/251‑1 (MQ=255)                                                                                                                                                                                                                                acCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGCTGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATAc                      >  1:70531/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCgg              >  1:102928/1‑250 (MQ=255)                                                                                                                                                                                                                                                    ccATCTGTTGCGTCAGGCGGCGGAAAAATCGCGGCGTAAATTCCGCTATGAC‑TCCAACGTGGGTGCTGGATTACCGGTTATTGAGATCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGGTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAgatg  <  2:73504/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       CTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATG  >  NC_000913/1861‑2353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑