Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,297	C→T	L303L (CTG→TTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,297	0	C	T	93.5%	200.4 / 2.1	79	L303L (CTG→TTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (46/26);  total (51/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

GCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTG  >  NC_000913/23051‑23543                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        gCACTGAAAGCAAAATTTTCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCGGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGTCGGGCAGGCCGTGATTCTGTCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTTCGGAGCTTGAgttgct                                                                                                                                                                                                                                                     <  2:399939/251‑1 (MQ=255)       aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACGACGCCGTCGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAATGTGCGGAGCTTGAGTTGCTGCGCtt                                                                                                                                                                                                                                               <  2:123954/251‑1 (MQ=255)          gCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAGTCTCTATTGCACCAGATTTCGACTATGCGTTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑Ac                                                                                                                                                                                                                                           >  1:47707/1‑251 (MQ=255)           cAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTCCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGAGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑Acc                                                                                                                                                                                                                                          >  1:225499/1‑251 (MQ=255)           cAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑Acc                                                                                                                                                                                                                                          <  1:255105/251‑1 (MQ=255)            aaaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGGTTACCCAATTTTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTT‑Accc                                                                                                                                                                                                                                         >  2:101602/1‑251 (MQ=255)              aaTTTGCCGTAAGCAACGTTAACGGCCCAATCGCGCTGGTTATCTGTACCACTACGCTGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGTATGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACTGGAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCAt                                                                                                                                                                                                                                       <  1:53061/251‑1 (MQ=255)                     cGTAAGCAACGTTAACGGCCCAATCTCGCTGGCGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGCCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTAAACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGttt                                                                                                                                                                                                                                 >  2:25972/1‑250 (MQ=255)                              cGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCg                                                                                                                                                                                                                       >  1:423073/1‑251 (MQ=255)                                    cGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTc                                                                                                                                                                                                                 <  1:278557/251‑1 (MQ=255)                                    cGGCCCAATCTCGCTGGGGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTc                                                                                                                                                                                                                 <  2:220551/251‑1 (MQ=255)                                      gCCCAATCTCGCTGGTGAGCTGGACCCCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCg                                                                                                                                                                                                               <  2:397928/251‑1 (MQ=255)                                               cccgccgctcttccgatcttacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                                                                                                                                                      <  2:33242/231‑1 (MQ=255)                                               cGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGTCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                                                                                                                                                      <  1:11877/251‑1 (MQ=255)                                                gCTGGTGATCTGGACCATGACGCCGTGTACGCTGCCTGCGAACCGCGCAATCTCTAGTGCTCCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTTTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCg                                                                                                                                                                                                     <  1:328995/251‑1 (MQ=255)                                                  tGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAATACACCATTCTCAGCACGGTACAAGGTGCGGAGCTTGAGTTGATGCGCTTTCACCC‑TCCGTTTATGGGCTTCGGCGATCCGGCAATCCTCGGc                                                                                                                                                                                                   >  2:395486/1‑251 (MQ=255)                                                       aTCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAACGATTACCCCATTCTCGGCACGGTAAAAGGGGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGGCGTTCGGGCAATCCCCGGCgctcc                                                                                                                                                                                              >  1:111058/1‑247 (MQ=255)                                                          tGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAACGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTACGCACGGTTAAAGGGGCGGAGGCTGGGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGATTCGGCGTTCCGGCAATCCTCGGCGATCACGt                                                                                                                                                                                           >  2:367104/1‑251 (MQ=255)                                                             accactACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGAAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATACGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCAATCACGTTAc                                                                                                                                                                                        >  1:474640/1‑251 (MQ=255)                                                                  tacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                                                                                                                                                      >  1:33242/2‑232 (MQ=255)                                                                        gTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCCCCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAACGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGGTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGAAATCCCGGGCGATCACGTTAACCTGGATGCCg                                                                                                                                                                             >  2:24608/1‑251 (MQ=255)                                                                         tGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAAGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCgg                                                                                                                                                                            >  1:359047/1‑251 (MQ=255)                                                                          ggACTCTGCCTGCGAACCGCGCAATCTCTAGTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGt                                                                                                                                                                           <  2:379488/251‑1 (MQ=255)                                                                             ccctgcctgcGAACCGCGCAATCGCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAcc                                                                                                                                                                        <  2:412669/249‑1 (MQ=255)                                                                                         aCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTcac                                                                                                                                                            <  1:217186/251‑1 (MQ=255)                                                                                               cAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACAATTCTCAGCACGGTAAAAGGGGCGGGGCTTGAGTTGATGCGCTTT‑ACCCATCCGGTTATGGGCTTCGACGTTCCGGCAATCCTAGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACcgcc                                                                                                                                                      >  2:80285/1‑250 (MQ=255)                                                                                                   ctctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTg                                                                                                                                                  >  1:338702/1‑251 (MQ=255)                                                                                                    tctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGGTTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTgg                                                                                                                                                 >  1:419814/1‑251 (MQ=255)                                                                                                    tctATTGCACCTGATTTCGACTATGCGCTGGCGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGCCCGATTCCCCCATTATCAGCACGGTAAAAGGTGCGGGGCATGAGTTGCTGCGCTTT‑ACCCACCCGTTTATGGGCTTCGACCTTCACGCCATCCTCGGCGCTCACGCTACCCTGGATGCCGGCAACGGTGCCGTTCCCACCGCGCCt                                                                                                                                                   >  2:423945/1‑249 (MQ=255)                                                                                                     ctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGc                                                                                                                                                >  1:392036/1‑251 (MQ=255)                                                                                                         tGCACCTGATTTCGACTATGCGCTGGTGCAGATCTCCGGTGAGGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAACGATTACAACATTCTCAGCACGGTAAAAGGTGCGGCGCTTTCGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCCCGCCGATCACGTTACCCTGGTTGCCGATACCGGTGCCGGTCACACAGCGCCCGGccccg                                                                                                                                            >  2:475728/1‑248 (MQ=255)                                                                                                              ctgATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCCACGTTCCGGCAATCCTCGGCGGTCACGTTACCCTGGAAGCCGGTACCGGTGCCGTTCACACCTCCCCTGGCCCCGGCCCg                                                                                                                                       >  2:427163/3‑251 (MQ=255)                                                                                                                  tttCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGTCAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGAGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCCGTCACCCCGCGCCTGGCCCCGGCCCgagcg                                                                                                                                   >  2:439013/1‑247 (MQ=255)                                                                                                                   ttCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACAGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacgg                                                                                                                                  >  1:249766/1‑250 (MQ=255)                                                                                                                          attcgcgggTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtg                                                                                                                             <  2:357835/242‑1 (MQ=255)                                                                                                                          aTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtgtg                                                                                                                           <  1:427163/251‑1 (MQ=255)                                                                                                                                 ggTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATc                                                                                                                        <  1:490821/247‑1 (MQ=255)                                                                                                                                 ggTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGAGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCgggcggccatgggatc                                                                                                                        >  2:490821/1‑233 (MQ=255)                                                                                                                                     cAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGGGATCGGTCAGa                                                                                                                 >  1:118366/1‑250 (MQ=255)                                                                                                                                             cGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACGATGTGATCGGTCAGAAATACGGcc                                                                                                        <  2:338687/251‑1 (MQ=255)                                                                                                                                                  aGGCCGTGATTCGGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaa                                                                                                   <  2:482778/251‑1 (MQ=255)                                                                                                                                                      cGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAAGACGGCCTGGAAACCg                                                                                               <  1:378526/251‑1 (MQ=255)                                                                                                                                                        tGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTTTGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCt                                                                                             >  1:380472/1‑251 (MQ=255)                                                                                                                                                          aTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTa                                                                                            >  1:404583/1‑250 (MQ=255)                                                                                                                                                             cTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTcc                                                                                                                                                                                                                <  1:334843/131‑1 (MQ=255)                                                                                                                                                             cTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTcc                                                                                                                                                                                                                >  2:334843/1‑131 (MQ=255)                                                                                                                                                               ggCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAACAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAAcc                                                                                         <  2:8002/248‑1 (MQ=255)                                                                                                                                                                  gAAAGCTCTGGTTGAAAGAGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACACTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGGGATCGGTAAGAAATACGGCCCGGAAACCGGTAAACAGGTTg                                                                                   >  2:321888/1‑251 (MQ=255)                                                                                                                                                                   aaaGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑AACCATCCGTTTATTGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTTCCCTGGATGCCGGTACCGGTTCCGTTATCACCGCTCCTGGCCACGGCCCGGACGTCTATGTTCTCGGTCAGAAATACAGCCTGGAACCCGCTAACCCTGTTgg                                                                                  >  2:488274/1‑251 (MQ=255)                                                                                                                                                                    aaGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGGCTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGc                                                                                 >  1:430931/1‑251 (MQ=255)                                                                                                                                                                     aGATCTGGTTGAAATCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGCGCTTGCGTTGCTGCGCTTT‑ACCCATCCGTTTATGGCCTTCGACGCTCCGGCAATCCTCGGCGATCCCCTTACCCTGGATGCCGGTACCGGTTACGTTCACACCGCGCCTGGCCACGGCCCGGACCACTATGTGGTCGGTCAGAAATACGGCCCTGCAACCGCTCACCCGGTTGGcc                                                                                >  1:457993/1‑251 (MQ=255)                                                                                                                                                                      gATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGccc                                                                               >  1:448336/1‑251 (MQ=255)                                                                                                                                                                           ggTTGAAAGGTTAATGCAGCGTATCGGCGTTACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTAGGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGccc                                                                               <  2:496499/246‑1 (MQ=255)                                                                                                                                                                               gAAAGCGGAATGCAGCGTATCGTCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTTCTGCGCTTT‑ACCCATCCGTTTATGGTCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAAGACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCAc                                                                      <  2:422816/251‑1 (MQ=255)                                                                                                                                                                                    cGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGCGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCCCCCCGCGCCTGGCCACGGCCCGGACGACTCTGTGATCGGTCAGAAATACCGCCCGGAAACCGCTAACCCGGTTGGCCCGGGCGGCACTTATc                                                                 >  2:272484/1‑251 (MQ=255)                                                                                                                                                                                    cGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATc                                                                 >  1:127754/1‑251 (MQ=255)                                                                                                                                                                                      tAATGCAGCGTATCGGCGTGACCGAATACACCATTCTGAGCCCGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACCCCGCCCGGCCGCGGCCCGGACGACTATGTGAGCGGTCAGAAATACGGCCTGGAACCCCCTAACACGGTTGGCCCGGACGGAACTTATcgg                                                               >  1:365430/1‑249 (MQ=255)                                                                                                                                                                                       aaTGCAGCGTATCGGCGTGACCGATTAAACCATTCTCAGCACGGTAAAAGGAGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGCTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACTGCCTGGAAACCGCTAACCCGGGTGGCCCGGACGGCACTTATCTGc                                                              >  1:332827/1‑251 (MQ=255)                                                                                                                                                                                        aTGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACCGTACAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGATACCCTGGAAGCCGGTACCGGCGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGGCTATGTGATCGGGCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGGCCTTATCTGcc                                                             >  1:396854/1‑251 (MQ=255)                                                                                                                                                                                                   cGGCGTGACCGATTACCCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTAt                                                   <  2:153986/250‑1 (MQ=255)                                                                                                                                                                                                   cGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGGACTTATc                                                  >  1:182285/1‑251 (MQ=255)                                                                                                                                                                                                      cGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCg                                                >  1:384659/1‑250 (MQ=255)                                                                                                                                                                                                       gTGACCGATTACACCATTCCCAGAACGGTAAAAGGTGTGGAGCTTGAGTTGCTGCGCTTT‑CCCCATCCGTTTATGGGCGTCGACGTTCCGGCAATCCGCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGAc                                              <  2:445739/251‑1 (MQ=255)                                                                                                                                                                                                           ccGATTACTCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTGGCTGCGCTTT‑ACCCTTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTTACACCGCGCCTGGCCACGGCCCGGACGACTATTTGATCGGTCAGAAATACGGCCTGGAAACCTCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTg                                          <  1:295447/251‑1 (MQ=255)                                                                                                                                                                                                            cGATTACCCCATTCCCGGCACGGTAAAAGGTGCGGAGCTTGAGCGGCTGCGCTTT‑ACCCACCCTGTTAGGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTCCCCTGGATGCCGGTACCGGTGCCGTTCACGCCGCGCCTGGCCACGGCCCGGACGACTATGTGAGCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCGCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTgg                                         <  2:47707/251‑1 (MQ=255)                                                                                                                                                                                                                    ccATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACTCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTg                                 >  1:6885/1‑251 (MQ=255)                                                                                                                                                                                                                           cagCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCt                          >  1:210846/3‑251 (MQ=255)                                                                                                                                                                                                                              cACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGAAGCTGGAAGGCGTGAACGTCTTc                        >  1:375834/1‑250 (MQ=255)                                                                                                                                                                                                                              cACGGCAAAAGATGCGGAGCTAGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGGGATCGGTCAGAAATACGGCCTGGAGCACGCTAACCCGGTTGGCCCGGACGGCACTTATCTTCCGGGCACTTATCCGCCGCTGGATGGAGTGGACGTCTTc                        >  2:360500/1‑250 (MQ=255)                                                                                                                                                                                                                                 ggTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGGTCGGTCAGACATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGCCGCGGGCTGGCGAGAACGACTTCaagg                    >  2:357975/1‑249 (MQ=255)                                                                                                                                                                                                                                 ggTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAg                    <  1:25972/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggTAAAAGGCGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGAGATCACGTTCCCCTGACAGCCGGTCCCGGTGCCGTTCCCACCCCACCTGGCCACGCACCGGACGACTATGGGATCGGGCCGACATACAGCCTGGACACCGATACCCCCGATGGCCCGCAAGGCACTTATCTGCCGTGCACTTATCAGACACTGGGTGGCGTGAACAACTTCaacg                    >  2:286426/1‑249 (MQ=255)                                                                                                                                                                                                                                    aaaaGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTACCCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCACAGCg                  >  2:184033/1‑250 (MQ=255)                                                                                                                                                                                                                                    aaaaGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGAAGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGCCTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCg                  >  1:483235/1‑250 (MQ=255)                                                                                                                                                                                                                                     aaaGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGCTCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCAGGAAACCGCTAACCCGGTTGGACCGGAAGGCACCTATCTGCCGGGCAATTATCCGACCCTGGATGGCGGGAACGTCTTCAAAGCGa                 >  2:402765/1‑250 (MQ=255)                                                                                                                                                                                                                                     aaaGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGAAGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGCCTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGCCGGCCCTTATCTGCCGGGCACTTATACGCCGCTGGATGGCGTGATCGTCTTCAAAGCGa                 >  2:231656/1‑250 (MQ=255)                                                                                                                                                                                                                                       aGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCAATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCATGGCCACGGCCCGGACGAATATGTGATCGGTCAGAAATACAGCCTGGAAAACGCTAACACGGTTGGCCCGGACGGCCCTTATATGCCGGGCACTTATCCGACGCTGGTTGGAGTGAACGTCTTCCGAGCGAACg              >  2:433753/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTGCGGAGCTTGAGATGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGAc            >  1:373539/1‑251 (MQ=255)                                                                                                                                                                                                                                                   tGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGtt   <  1:184033/250‑1 (MQ=255)                                                                                                                                                                                                                                                   tGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTg  >  1:117740/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        GCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTG  >  NC_000913/23051‑23543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑