Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,270	G→A	G294S (GGC→AGC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,270	0	G	A	94.7%	207.8 / ‑1.9	77	G294S (GGC→AGC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (41/30);  total (45/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

GCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTG  >  NC_000913/23027‑23512                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    gCTTTTCAGGCGGTCGATCAGGATGCCCTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACgg                                                                                                                                                                                                                                               <  2:32924/250‑1 (MQ=255)     ttcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTaaa                                                                                                                                                                                                                                           >  1:445739/3‑250 (MQ=255)       cAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAg                                                                                                                                                                                                                                         <  1:243926/250‑1 (MQ=255)          gcggTCGATCAGGAGGCACTAAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACGACGTCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGTCGAAATATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGc                                                                                                                                                                                                                                     <  2:45915/248‑1 (MQ=255)                gATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCt                                                                                                                                                                                                                               >  1:338687/1‑251 (MQ=255)                   cAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTg                                                                                                                                                                                                                             <  1:362273/250‑1 (MQ=255)                     ggATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCCTTCTCAGCACGGGAAAAGGTGCGGAGCGTGAgt                                                                                                                                                                                                                          >  1:265810/1‑250 (MQ=255)                         gCACTGAAAGCAAAATTTTCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCGGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGTCGGGCAGGCCGTGATTCTGTCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTTCGGAGCTTGAgttgct                                                                                                                                                                                                                      <  2:399939/251‑1 (MQ=255)                             tGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTAGCTGGTGATCTGGACCACTACGCAGTGGACTATGCCTGCGAACCGCGCACTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTTGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATAGGCGTGCCCGACTACACCATTCTCAGCACGGCAAAAggggaggggcttggactgtcgcgc                                                                                                                                                                                                                  >  2:215072/1‑229 (MQ=255)                               aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCAGCACGGTAAAAGGTg                                                                                                                                                                                                                                      >  2:423572/1‑229 (MQ=255)                               aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTg                                                                                                                                                                                                                                      <  1:423572/229‑1 (MQ=255)                               aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACGACGCCGTCGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAATGTGCGGAGCTTGAGTTGCTGCGCtt                                                                                                                                                                                                                <  2:123954/251‑1 (MQ=255)                                  gCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAGTCTCTATTGCACCAGATTTCGACTATGCGTTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑Ac                                                                                                                                                                                                            >  1:47707/1‑251 (MQ=255)                                   cAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTCCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGAGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑Acc                                                                                                                                                                                                           >  1:225499/1‑251 (MQ=255)                                   cAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑Acc                                                                                                                                                                                                           <  1:255105/251‑1 (MQ=255)                                    aaaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGGTTACCCAATTTTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTT‑Accc                                                                                                                                                                                                          >  2:101602/1‑251 (MQ=255)                                      aaTTTGCCGTAAGCAACGTTAACGGCCCAATCGCGCTGGTTATCTGTACCACTACGCTGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGTATGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACTGGAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCAt                                                                                                                                                                                                        <  1:53061/251‑1 (MQ=255)                                             cGTAAGCAACGTTAACGGCCCAATCTCGCTGGCGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGCCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTAAACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGttt                                                                                                                                                                                                  >  2:25972/1‑250 (MQ=255)                                                      cGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCg                                                                                                                                                                                        >  1:423073/1‑251 (MQ=255)                                                         tAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACAATTCTCAGCACGGTAAAAGGTGCGGAGCtt                                                                                                                                                                                                                              >  1:346162/1‑211 (MQ=255)                                                            cGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTc                                                                                                                                                                                  <  1:278557/251‑1 (MQ=255)                                                            cGGCCCAATCTCGCTGGGGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTc                                                                                                                                                                                  <  2:220551/251‑1 (MQ=255)                                                              gCCCAATCTCGCTGGTGAGCTGGACCCCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCg                                                                                                                                                                                <  2:397928/251‑1 (MQ=255)                                                                       cccgccgctcttccgatcttacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                                                                                                                       <  2:33242/231‑1 (MQ=255)                                                                       cGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGTCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                                                                                                                       <  1:11877/251‑1 (MQ=255)                                                                        gCTGGTGATCTGGACCATGACGCCGTGTACGCTGCCTGCGAACCGCGCAATCTCTAGTGCTCCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTTTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCg                                                                                                                                                                      <  1:328995/251‑1 (MQ=255)                                                                          tGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAATACACCATTCTCAGCACGGTACAAGGTGCGGAGCTTGAGTTGATGCGCTTTCACCC‑TCCGTTTATGGGCTTCGGCGATCCGGCAATCCTCGGc                                                                                                                                                                    >  2:395486/1‑251 (MQ=255)                                                                               aTCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAACGATTACCCCATTCTCGGCACGGTAAAAGGGGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGGCGTTCGGGCAATCCCCGGCgctcc                                                                                                                                                               >  1:111058/1‑247 (MQ=255)                                                                                  tGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAACGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTACGCACGGTTAAAGGGGCGGAGGCTGGGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGATTCGGCGTTCCGGCAATCCTCGGCGATCACGt                                                                                                                                                            >  2:367104/1‑251 (MQ=255)                                                                                     accactACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGAAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATACGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCAATCACGTTAc                                                                                                                                                         >  1:474640/1‑251 (MQ=255)                                                                                          tacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                                                                                                                       >  1:33242/2‑232 (MQ=255)                                                                                                gTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCCCCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAACGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGGTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGAAATCCCGGGCGATCACGTTAACCTGGATGCCg                                                                                                                                              >  2:24608/1‑251 (MQ=255)                                                                                                 tGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAAGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCgg                                                                                                                                             >  1:359047/1‑251 (MQ=255)                                                                                                  ggACTCTGCCTGCGAACCGCGCAATCTCTAGTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGt                                                                                                                                            <  2:379488/251‑1 (MQ=255)                                                                                                     ccctgcctgcGAACCGCGCAATCGCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAcc                                                                                                                                         <  2:412669/249‑1 (MQ=255)                                                                                                                 aCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTcac                                                                                                                             <  1:217186/251‑1 (MQ=255)                                                                                                                       cAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACAATTCTCAGCACGGTAAAAGGGGCGGGGCTTGAGTTGATGCGCTTT‑ACCCATCCGGTTATGGGCTTCGACGTTCCGGCAATCCTAGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACcgcc                                                                                                                       >  2:80285/1‑250 (MQ=255)                                                                                                                           ctctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTg                                                                                                                   >  1:338702/1‑251 (MQ=255)                                                                                                                            tctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGGTTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTgg                                                                                                                  >  1:419814/1‑251 (MQ=255)                                                                                                                            tctATTGCACCTGATTTCGACTATGCGCTGGCGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGCCCGATTCCCCCATTATCAGCACGGTAAAAGGTGCGGGGCATGAGTTGCTGCGCTTT‑ACCCACCCGTTTATGGGCTTCGACCTTCACGCCATCCTCGGCGCTCACGCTACCCTGGATGCCGGCAACGGTGCCGTTCCCACCGCGCCt                                                                                                                    >  2:423945/1‑249 (MQ=255)                                                                                                                             ctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGc                                                                                                                 >  1:392036/1‑251 (MQ=255)                                                                                                                                 tGCACCTGATTTCGACTATGCGCTGGTGCAGATCTCCGGTGAGGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAACGATTACAACATTCTCAGCACGGTAAAAGGTGCGGCGCTTTCGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCCCGCCGATCACGTTACCCTGGTTGCCGATACCGGTGCCGGTCACACAGCGCCCGGccccg                                                                                                             >  2:475728/1‑248 (MQ=255)                                                                                                                                      ctgATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCCACGTTCCGGCAATCCTCGGCGGTCACGTTACCCTGGAAGCCGGTACCGGTGCCGTTCACACCTCCCCTGGCCCCGGCCCg                                                                                                        >  2:427163/3‑251 (MQ=255)                                                                                                                                          tttCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGTCAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGAGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCCGTCACCCCGCGCCTGGCCCCGGCCCgagcg                                                                                                    >  2:439013/1‑247 (MQ=255)                                                                                                                                           ttCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACAGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacgg                                                                                                   >  1:249766/1‑250 (MQ=255)                                                                                                                                                  attcgcgggTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtg                                                                                              <  2:357835/242‑1 (MQ=255)                                                                                                                                                  aTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtgtg                                                                                            <  1:427163/251‑1 (MQ=255)                                                                                                                                                         ggTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATc                                                                                         <  1:490821/247‑1 (MQ=255)                                                                                                                                                         ggTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGAGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCgggcggccatgggatc                                                                                         >  2:490821/1‑233 (MQ=255)                                                                                                                                                             cAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGGGATCGGTCAGa                                                                                  >  1:118366/1‑250 (MQ=255)                                                                                                                                                                     cGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACGATGTGATCGGTCAGAAATACGGcc                                                                         <  2:338687/251‑1 (MQ=255)                                                                                                                                                                          aGGCCGTGATTCGGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaa                                                                    <  2:482778/251‑1 (MQ=255)                                                                                                                                                                              cGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAAGACGGCCTGGAAACCg                                                                <  1:378526/251‑1 (MQ=255)                                                                                                                                                                                tGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTTTGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCt                                                              >  1:380472/1‑251 (MQ=255)                                                                                                                                                                                  aTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTa                                                             >  1:404583/1‑250 (MQ=255)                                                                                                                                                                                     cTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTcc                                                                                                                                                                                 <  1:334843/131‑1 (MQ=255)                                                                                                                                                                                     cTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTcc                                                                                                                                                                                 >  2:334843/1‑131 (MQ=255)                                                                                                                                                                                       ggCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAACAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAAcc                                                          <  2:8002/248‑1 (MQ=255)                                                                                                                                                                                          gAAAGCTCTGGTTGAAAGAGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACACTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGGGATCGGTAAGAAATACGGCCCGGAAACCGGTAAACAGGTTg                                                    >  2:321888/1‑251 (MQ=255)                                                                                                                                                                                           aaaGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑AACCATCCGTTTATTGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTTCCCTGGATGCCGGTACCGGTTCCGTTATCACCGCTCCTGGCCACGGCCCGGACGTCTATGTTCTCGGTCAGAAATACAGCCTGGAACCCGCTAACCCTGTTgg                                                   >  2:488274/1‑251 (MQ=255)                                                                                                                                                                                            aaGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGGCTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGc                                                  >  1:430931/1‑251 (MQ=255)                                                                                                                                                                                             aGATCTGGTTGAAATCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGCGCTTGCGTTGCTGCGCTTT‑ACCCATCCGTTTATGGCCTTCGACGCTCCGGCAATCCTCGGCGATCCCCTTACCCTGGATGCCGGTACCGGTTACGTTCACACCGCGCCTGGCCACGGCCCGGACCACTATGTGGTCGGTCAGAAATACGGCCCTGCAACCGCTCACCCGGTTGGcc                                                 >  1:457993/1‑251 (MQ=255)                                                                                                                                                                                              gATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGccc                                                >  1:448336/1‑251 (MQ=255)                                                                                                                                                                                                   ggTTGAAAGGTTAATGCAGCGTATCGGCGTTACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTAGGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGccc                                                <  2:496499/246‑1 (MQ=255)                                                                                                                                                                                                       gAAAGCGGAATGCAGCGTATCGTCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTTCTGCGCTTT‑ACCCATCCGTTTATGGTCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAAGACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCAc                                       <  2:422816/251‑1 (MQ=255)                                                                                                                                                                                                            cGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGCGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCCCCCCGCGCCTGGCCACGGCCCGGACGACTCTGTGATCGGTCAGAAATACCGCCCGGAAACCGCTAACCCGGTTGGCCCGGGCGGCACTTATc                                  >  2:272484/1‑251 (MQ=255)                                                                                                                                                                                                            cGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATc                                  >  1:127754/1‑251 (MQ=255)                                                                                                                                                                                                              tAATGCAGCGTATCGGCGTGACCGAATACACCATTCTGAGCCCGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACCCCGCCCGGCCGCGGCCCGGACGACTATGTGAGCGGTCAGAAATACGGCCTGGAACCCCCTAACACGGTTGGCCCGGACGGAACTTATcgg                                >  1:365430/1‑249 (MQ=255)                                                                                                                                                                                                               aaTGCAGCGTATCGGCGTGACCGATTAAACCATTCTCAGCACGGTAAAAGGAGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGCTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACTGCCTGGAAACCGCTAACCCGGGTGGCCCGGACGGCACTTATCTGc                               >  1:332827/1‑251 (MQ=255)                                                                                                                                                                                                                aTGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACCGTACAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGATACCCTGGAAGCCGGTACCGGCGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGGCTATGTGATCGGGCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGGCCTTATCTGcc                              >  1:396854/1‑251 (MQ=255)                                                                                                                                                                                                                           cGGCGTGACCGATTACCCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTAt                    <  2:153986/250‑1 (MQ=255)                                                                                                                                                                                                                           cGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGGACTTATc                   >  1:182285/1‑251 (MQ=255)                                                                                                                                                                                                                              cGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCg                 >  1:384659/1‑250 (MQ=255)                                                                                                                                                                                                                               gTGACCGATTACACCATTCCCAGAACGGTAAAAGGTGTGGAGCTTGAGTTGCTGCGCTTT‑CCCCATCCGTTTATGGGCGTCGACGTTCCGGCAATCCGCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGAc               <  2:445739/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccGATTACTCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTGGCTGCGCTTT‑ACCCTTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTTACACCGCGCCTGGCCACGGCCCGGACGACTATTTGATCGGTCAGAAATACGGCCTGGAAACCTCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTg           <  1:295447/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGATTACCCCATTCCCGGCACGGTAAAAGGTGCGGAGCTTGAGCGGCTGCGCTTT‑ACCCACCCTGTTAGGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTCCCCTGGATGCCGGTACCGGTGCCGTTCACGCCGCGCCTGGCCACGGCCCGGACGACTATGTGAGCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCGCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTgg          <  2:47707/251‑1 (MQ=255)                                                                                                                                                                                                                                            ccATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACTCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTg  >  1:6885/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTG  >  NC_000913/23027‑23512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑