Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,032	C→T	F214F (TTC→TTT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,032	0	C	T	95.2%	180.4 / ‑1.2	63	F214F (TTC→TTT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (28/32);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTC‑CAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGG  >  NC_000913/22786‑23276                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAgg                                                                                                                                                                                                                                                  >  2:265486/1‑251 (MQ=255)      ttGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGGGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAggtggtc                                                                                                                                                                                                                                             >  2:356569/1‑246 (MQ=255)            gTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGAGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATc                                                                                                                                                                                                                                         >  2:448187/1‑249 (MQ=255)                           ttATCCGGCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGc                                                                                                                                                                                                                         <  2:356107/250‑1 (MQ=255)                             aTCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCCTGGACTTCAAAACTGAAGCCACCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACCCTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAACGGACGTTGAGTCTTCCGACAAAACTTCTCCGTCCATCTACGTTGCTTTT‑CAGTCGGTCGATCAGGATGCACTGAAAGCaa                                                                                                                                                                                                                       >  2:243926/1‑250 (MQ=255)                             aTCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCaa                                                                                                                                                                                                                       >  1:425430/1‑250 (MQ=255)                                     gggCGTGCTGGGCGACTGGTTGCACTCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTg                                                                                                                                                                                                                 <  2:292874/248‑1 (MQ=255)                                         gTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGACGCCAACATCATCCGCGCGCTGGGCAAACTCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGAGCGTTGACTGCCGTTCTGCGCTGGCGGCAACGGACGTTGAGTCTTACGACAACACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACCGAAAGCAACAtttacctta                                                                                                                                                                                                            >  2:320997/1‑243 (MQ=255)                                         gTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTa                                                                                                                                                                                                            >  1:229796/1‑249 (MQ=255)                                             tGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCGAAACTGAAGCCACCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGCAGTTGAGTATTACGACAACACTTCTCCGTCCATCGACGTTGCTTTC‑CAGGCCGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAAc                                                                                                                                                                                                      >  2:255105/1‑251 (MQ=255)                                             tGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAAc                                                                                                                                                                                                      >  1:120092/1‑251 (MQ=255)                                              gggcgtatGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGGGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCaa                                                                                                                                                                                                       <  2:348562/242‑1 (MQ=255)                                              gggCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAAc                                                                                                                                                                                                      <  1:303790/250‑1 (MQ=255)                                                            aCCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGATGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTTGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAATCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAtc                                                                                                                                                                                       <  1:369689/251‑1 (MQ=255)                                                              ccGTACCTGACCATGGACTTCAAAACGGAAGCCAACTTCAGCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCGGCACAAAGGCGCGAAGCCGGTACACGGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTGATCGGCCCAAtcgc                                                                                                                                                                                     <  2:231977/251‑3 (MQ=255)                                                                gTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGt                                                                                                                                                                                                    >  1:55836/1‑234 (MQ=255)                                                                gTACCTGACCATGGACTTCAAAACGGAAGCCAACATCATCCGCGCGCTGGGCAAAATCAGCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGt                                                                                                                                                                                                    <  2:55836/234‑1 (MQ=255)                                                                         cATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGAAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCt                                                                                                                                                                          >  1:281383/1‑251 (MQ=255)                                                                            ggaattCAAAACTGAAGCCAACATCAACCGCGCGCTGGCCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGACGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGAGCTGGa                                                                                                                                                                       <  2:269656/247‑1 (MQ=255)                                                                             gACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGTCAAAAGCATCGGCAACGGTCACCTGCCCAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATGTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGac                                                                                                                                                                      <  2:500961/251‑1 (MQ=255)                                                                                ttCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGaccac                                                                                                                                                                   >  2:217186/1‑251 (MQ=255)                                                                                 tCAAAACGGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACGTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGaccac                                                                                                                                                                   <  2:393554/250‑1 (MQ=255)                                                                                        tGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGt                                                                                                                                                           >  1:8002/1‑251 (MQ=255)                                                                                         gAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTg                                                                                                                                                          <  1:65444/251‑1 (MQ=255)                                                                                           aGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTgg                                                                                                                                                         >  2:251561/1‑250 (MQ=255)                                                                                                        cgcgcgCTGGGCAAACTCACCGGTAACGGTCACCTGTGCAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAACCTGCTCCGTCCATCGACGTTG‑TTTCTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCTCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTctgcctgcgaa                                                                                                                                           <  1:20772/251‑4 (MQ=255)                                                                                                            cgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTAAGCCGTGGACTCTGCCTGCGAACcgc                                                                                                                                       >  1:496499/1‑251 (MQ=255)                                                                                                                gggCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTC‑CAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCC‑AATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAAt                                                                                                                                  <  2:237385/251‑1 (MQ=255)                                                                                                                 ggCAAAATCATCGGCATCTGGCCCCTGCACAAAGGCGCGATGCCGGTACACTGGTGCGGTGACTGCCGTTCTGCGCTGGCGGAAGCTGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGGCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACTTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAATCGCGCAAt                                                                                                                                  <  2:400878/251‑1 (MQ=255)                                                                                                                         catcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTggggat                                                                                                                                                                            >  2:29508/1‑197 (MQ=255)                                                                                                                         catcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtgat                                                                                                                                                                            <  1:29508/201‑4 (MQ=255)                                                                                                                                         cTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACt                                                                                                           >  1:482778/1‑250 (MQ=255)                                                                                                                                           tcacaAAGGCGCGAAGCCGGTACACTGGTGCGTTGTCTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTATCCGTCCATCGACGGTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGGTATCGGCCCAAGCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCGGCCTGCGAACCGCGCAATCTCGTTTGCACTGGATTGCGACTAt                                                                                                         <  2:503381/249‑1 (MQ=255)                                                                                                                                               aaaGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCt                                                                                                    >  2:115229/1‑251 (MQ=255)                                                                                                                                               aaaGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCt                                                                                                    >  1:379488/1‑251 (MQ=255)                                                                                                                                                aaGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCCCCTGATTTCGCCTATGCGCTg                                                                                                   >  2:125876/1‑251 (MQ=255)                                                                                                                                                            cggtacACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACAGAAAGCAAAATTTGCCGTCAGAAAAGTTAACGGCCCAATCTCGCTGGTGATCTGTACCACTACGCCGTGGCATCTGCCTGCAAACCGCGCAATCTCTATTCCACCTGATTTCGACAATGCTCTGGAGCAGTTCGAc                                                                                       >  2:274235/6‑251 (MQ=255)                                                                                                                                                             ggtacACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACg                                                                                      <  1:265486/247‑1 (MQ=255)                                                                                                                                                             ggtacACTGGTGCGTTGACTGCCGTTCGGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACg                                                                                      <  2:149249/247‑1 (MQ=255)                                                                                                                                                                  aCTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGCCTATGCGATGGTGCAGATCGACGGTc                                                                                   >  1:368787/1‑249 (MQ=255)                                                                                                                                                                              aCTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTg                                                                     <  2:86781/251‑1 (MQ=255)                                                                                                                                                                              aCTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGAGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTg                                                                     >  2:306621/1‑251 (MQ=255)                                                                                                                                                                                      tCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAg                                                              <  1:251561/250‑1 (MQ=255)                                                                                                                                                                                         gcgcGGGCTGAAGCTGAAGTTTAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTTAAAGCAAAATTTGCCTTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTTTATTGCATCTGATTTCGACTATGCGCTGGTGCAGATCGTCGTTCAGGTCGTGATTCTGGCGAAAGATCt                                                          <  1:360000/251‑1 (MQ=255)                                                                                                                                                                                                   aagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAACATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGc                                                >  1:230879/1‑251 (MQ=255)                                                                                                                                                                                                        gaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCAGAAGATCTGTTTGaagacgcaat                                           >  2:450379/1‑243 (MQ=255)                                                                                                                                                                                                          agTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGc                                         >  1:117650/1‑251 (MQ=255)                                                                                                                                                                                                               attactACGACAAATCTTTTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGTATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTTGTGATCTGGACCACTACGCCGTGGACTCTGCCTTCGAACCGCGCAATCGCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGt                                    <  1:54215/246‑1 (MQ=255)                                                                                                                                                                                                               aggaTTACGACAAAACTTCTCCGTCCAACGTCGTTGCTGTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCCCTGGTGAGCTGGACCACTACGCCGTGGACTCTGCCGGCGAACCGCGCAATCTCTATTGCACCTGATTTCGAATATGCGCTGGTGCAGATCGACGGTCAGGTCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTACTGCAGCGt                                    <  2:351610/248‑1 (MQ=255)                                                                                                                                                                                                               aGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGt                                    <  2:425430/251‑1 (MQ=255)                                                                                                                                                                                                                           aaaCTTCTCCGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTggcc                        >  1:228682/1‑248 (MQ=255)                                                                                                                                                                                                                                   ccggccATCGACGTTTCTTTT‑CAGGCGGACGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGACCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCGGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGGATCTGCGTGACCGATTac                  <  2:225698/245‑1 (MQ=255)                                                                                                                                                                                                                                    cGTCCATCGACGTTGCTTTT‑CAGGCGGTCGGTCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTctg                                                                                                                                                   >  1:277680/1‑119 (MQ=255)                                                                                                                                                                                                                                    cGTCCATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc               <  1:78738/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGTCCAGCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTctg                                                                                                                                                   <  2:277680/119‑1 (MQ=255)                                                                                                                                                                                                                                     gtccctctaCGTTGCTTTT‑CAGGCGGTCGACCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTCTTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCTAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCa              <  2:281383/243‑1 (MQ=255)                                                                                                                                                                                                                                       ccattggcGTTGCTTTT‑CAGGCGGTCGATTAGGATGCACAGAAAGCAAAATTTTCCGTAAGCAACGTTAACGGCCCAATCTATCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCTCCGGAGTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCAt             <  2:278228/243‑1 (MQ=255)                                                                                                                                                                                                                                        cATCGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTTACCGATTACAcccttt           >  2:6420/1‑247 (MQ=255)                                                                                                                                                                                                                                         agcGACGGTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtct          <  1:267733/249‑1 (MQ=255)                                                                                                                                                                                                                                           cGACGTTGCTTTT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtct          <  1:356569/249‑1 (MQ=255)                                                                                                                                                                                                                                           cGACGTTGCGTTT‑CAGTCGGTCGATCAGGATGCACTGAAAGCAAAAGTTTCCGTAAGAAACGTTAACGGCCCAATCTCGCGGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAACTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATtctca        <  2:460447/251‑2 (MQ=255)                                                                                                                                                                                                                                             aCGTTGCTTGT‑CAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCGGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagc      <  1:74963/251‑4 (MQ=255)                                                                                                                                                                                                                                                  gCTTTT‑CAGGCGGTCGATCAGGATGCCCTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACgg  <  2:32924/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTC‑CAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGG  >  NC_000913/22786‑23276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑