Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	22,468	C→G	P26P (CCC→CCG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	22,468	0	C	G	93.5%	134.2 / 3.4	46	P26P (CCC→CCG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (21/22);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGTTATGACTCGCCGTATGTGCCTGGCTGGGACTGCCACGGTCTGCCGATCGAGCTGAAAGTCG  >  NC_000913/22223‑22709                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  cAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAAt                                                                                                                                                                                                                                              <  1:1494/251‑1 (MQ=255)  aaGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATg                                                                                                                                                                                                                                             >  2:362547/1‑251 (MQ=255)       gtGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGgc                                                                                                                                                                                                                                        >  1:288468/1‑251 (MQ=255)                         cGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTCAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatgatg                                                                                                                                                                                                                       >  1:153840/1‑250 (MQ=255)                          gCAAGGAGCAGCGATTTGCGTCGCCGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCTAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCGCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTgatgatgat                                                                                                                                                                                                                     <  2:153840/251‑1 (MQ=255)                                 gcagcGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTCGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACg                                                                                                                                                                                                              <  1:453002/251‑1 (MQ=255)                                     cGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGGATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGcat                                                                                                                                                                                                          <  1:395863/251‑1 (MQ=255)                                     cGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGcat                                                                                                                                                                                                          >  1:324258/1‑251 (MQ=255)                                           gCGTCGCTGGACGACCTGAAAGCGCAGATGGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTTACAAAACCGGCTTAAGCCTGTGATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGTGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGATTGATGATGATCTGTACGGCATCATCCg                                                                                                                                                                                                    <  2:241268/251‑1 (MQ=255)                                              gcGCTGGACGAACTGAAAGCGCAGATTTCGTGTGAGGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGTGAATCTGATGAGTGACTATAAAGCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCATGc                                                                                                                                                                                                 <  1:24917/250‑1 (MQ=255)                                                           tGAAAGCGCAGATTTCGCGTGATTAATTAACCGCCCGCGAAGTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGTAATGCTGGCTCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAgg                                                                                                                                                                                     <  2:439048/250‑1 (MQ=255)                                                            gAAAGCTCAGATTGCTCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGTAACCGAGAATCTGATGAGTGACTATAAATCAAACCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGTGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGTTAAAAAAGGc                                                                                                                                                                                    <  1:269540/250‑1 (MQ=255)                                                                 cgcAGCTTGCGCGTGATGAATTAACCGCCCGCGATTTTTTTGGGCTAACAAAACCGGCTTCAGCCTGTTATGAAATCAAACCGAAACACGGAACCGCGAATCTGATGAGTGACTATAAATCAACCCTGACTTTGCCGGAACAAGGGTTCCCGATGCTGGGCGATCTCGCAAAGCGCGAACCGGGAATGCTGGCACGTTGGACTGATGGTGATCTGTACGGCATCATCAGAGCGACTAAAAAAGACaaaaa                                                                                                                                                                               >  2:18707/1‑250 (MQ=255)                                                                     gATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACtgat                                                                                                                                                                                                                           <  1:61100/202‑1 (MQ=255)                                                                     gATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACtgat                                                                                                                                                                                                                           >  2:61100/1‑202 (MQ=255)                                                                     gATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGACTCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCt                                                                                                                                                                          >  1:335353/1‑251 (MQ=255)                                                                                                          gggCTAACAAAACCGGTGTAATCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCGTATGAGTGACTATAATTCAACCCTGAATTTGCCGTAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAAGGCTGGCGCGTTGGACTGATGATTATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCGGCATGATGGCCCTCCTTATTCGAATGgcag                                                                                                                                     <  2:453045/251‑1 (MQ=255)                                                                                                                   aaaCCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCatat                                                                                                                            >  1:231977/1‑251 (MQ=255)                                                                                                                        ggCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTc                                                                                                                       >  1:361843/1‑251 (MQ=255)                                                                                                                        ggCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTc                                                                                                                       >  1:160819/1‑251 (MQ=255)                                                                                                                              aGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCgg                                                                                                                 <  1:298324/251‑1 (MQ=255)                                                                                                                                                   gAAACACGGAACCGCGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGTGTTCACGATGCGTGGCGATCTCGCCAAGCGCGCACCGGGAATGCTGGCGCGATGGACTGATGTTCATCTGGACGGCATCATCCGTGCGGCTAAAACAGGCAAAACCACCTTCTTTCTGCATGTTGGCCCTCCTTATGCGAGTGGCAGCATTACTCTTGGTCACTCGATTAAAAATCTTCCGAAAGAc                                                                                             >  2:65444/1‑250 (MQ=255)                                                                                                                                                   gAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGt                                                                                                                        <  2:180992/223‑1 (MQ=255)                                                                                                                                                   gAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGt                                                                                                                        >  1:180992/1‑223 (MQ=255)                                                                                                                                                      acaCGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTa                                                                                         <  1:254097/249‑1 (MQ=255)                                                                                                                                                         cGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAAGTTGCCGGAAACAGGGGTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCGTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCg                                                                                      <  2:259520/251‑1 (MQ=255)                                                                                                                                                          ggAACCGAGCATCTGAAGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGt                                                                                     >  1:350929/1‑251 (MQ=255)                                                                                                                                                           gagccGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGATCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTg                                                                                    >  2:435198/4‑251 (MQ=255)                                                                                                                                                            aaCCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCTGAAACAGGGTTCCCGATGCGTGGCGAGCTCGCCAAGCTCGAACCGGGAATGCTGGCGCGGTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGa                                                                                   <  1:72497/251‑1 (MQ=255)                                                                                                                                                                   aaTCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCaa                                                                            <  2:288468/251‑1 (MQ=255)                                                                                                                                                                        gatgaGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAgg                                                                         <  1:272518/249‑1 (MQ=255)                                                                                                                                                                        gatgaGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAg                                                                          <  1:208432/248‑1 (MQ=255)                                                                                                                                                                               gACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCgg                                                                >  2:173440/1‑251 (MQ=255)                                                                                                                                                                                aCTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGt                                                               <  2:361843/251‑1 (MQ=255)                                                                                                                                                                                       aacAACCCTTAATTTGCCCGTAACGGGGTTCCCTATTGGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCTTTTGACTGATGATGATCTGTTCGGCATCAGCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCGGCATGATGGCCCTCCTTATGCGAAAGTCAGCAGTCATAGTGGTCACTCGGTTAACAAGATTCTGAAATACATTATCGTGAAGGTCAAAGGGCTTTCCGGTTATGACt                                                        <  2:488096/249‑1 (MQ=255)                                                                                                                                                                                          aaCCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCAtt                                                                                                                                                                     >  2:99657/1‑139 (MQ=255)                                                                                                                                                                                          aaCCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCAtt                                                                                                                                                                     <  1:99657/139‑1 (MQ=255)                                                                                                                                                                                          aaCCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGTTATGACTCGc                                                     >  1:32110/1‑251 (MQ=255)                                                                                                                                                                                             ccTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGTTATGACTCGCCGt                                                  >  1:484699/1‑251 (MQ=255)                                                                                                                                                                                                     tGCCGGCAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGACTGCTGGCGCGTTGGATTGATGCTGATCTGAACGGCATCATCCGTGCGGCTAACCAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGACTGGCAGCATTCATCTTGGTCACTCGGTTAACAAGATTATGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGTTATGACTCGCCGTATGTGCct                                          >  1:458596/1‑251 (MQ=255)                                                                                                                                                                                                      gCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGTTATGACTCGCCGTATGTGCctg                                         <  2:90628/251‑1 (MQ=255)                                                                                                                                                                                                       ccGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTTATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACAGTATCGTGAAGTCCAAAGGGCTTTCCGGTTATGACTCGCCGTATGTGCctgg                                        <  1:61877/251‑1 (MQ=255)                                                                                                                                                                                                              cAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGTTAGGACTCGCCGTATGTGCCTGGCTGGGAc                                 >  2:350171/1‑251 (MQ=255)                                                                                                                                                                                                                  gTTCCCGATGCATGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAAGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCTTATTGGTCACTCGGTTAACAAGATTCTGAAAGACCTTATCGTGAAGTCCAACGGGCTTTCCGGGTATGACTCGCCGTATGTGCCTGGCTGGGACTGcc                             >  2:451241/1‑251 (MQ=255)                                                                                                                                                                                                                        gATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGTTATGACTCGCCGAATGTGCCTGGCTGGGACTGCCACGGTc                       >  2:353045/1‑251 (MQ=255)                                                                                                                                                                                                                                              cgcgAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGAATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGTTATGACTCGCCGTATGTGCCTGGCTGGGACTGCCACGGTCTGCCGATCGAGCTGAAAGTCg  >  1:425156/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGACATTATCGTGAAGTCCAAAGGGCTTTCCGGTTATGACTCGCCGTATGTGCCTGGCTGGGACTGCCACGGTCTGCCGATCGAGCTGAAAGTCG  >  NC_000913/22223‑22709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑