Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	22,373	T→C	intergenic (+25/‑18)	ribF → / → ileS	bifunctional riboflavin kinase/FMN adenylyltransferase/isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	22,373	0	T	C	92.3%	102.7 / ‑1.0	40	intergenic (+25/‑18)	ribF/ileS	bifunctional riboflavin kinase/FMN adenylyltransferase/isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (17/19);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-02

GCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGAC  >  NC_000913/22127‑22618                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      gCAAACATCGGAACACGCCCAATGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATTTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGTGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACgg                                                                                                                                                                                                                                                   <  1:263599/251‑1 (MQ=255)    aaCATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAAc                                                                                                                                                                                                                                                <  2:245161/251‑1 (MQ=255)                 gCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGTCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGGCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCtgatg                                                                                                                                                                                                                                   <  1:276991/251‑1 (MQ=255)                 gCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAA‑TTCGGAATGAGCCACGGTTTGCGGCGCTGGACGGACTGAAAGCGCAGGTTGCGCGGGATGGATTAACCGCCCGCGGATTTTTTTGGCTTACAAAACCGGCTTAAAGCTGTTATGTAATTAAACCGGAATACGGAACCGAGAATCtgatg                                                                                                                                                                                                                                   >  1:40412/1‑250 (MQ=255)                               ggTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATc                                                                                                                                                                                                                     <  1:263304/251‑1 (MQ=255)                                           cagcTGGAAGTGCAGTTGTTAGATGGTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAAttt                                                                                                                                                                                                         <  2:83272/251‑1 (MQ=255)                                                  aaGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACACGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAAAACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGa                                                                                                                                                                                                   >  1:69105/1‑250 (MQ=255)                                                     tGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAAc                                                                                                                                                                                                >  1:90628/1‑250 (MQ=255)                                                         tttGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGCACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGt                                                                                                                                                                                           >  2:16141/1‑251 (MQ=255)                                                            gttgaaTGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCTTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTTAATTTGCCGGAACCAGGGTTc                                                                                                                                                                                         <  2:290018/245‑1 (MQ=255)                                                                            gcactctacagacgcgatatACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGACGCTGATCGCACTAAAAGCGCAGATTGCACGTGATGAATTAACCGCGCGAGCATTTTTTGGGCTAACAAAACCGGATTAAGCCTGATATGCAATCAAACCGAAACACGGAATCCTGCATCTGCTAAGATACTATAAATCAACCCTGACTTTGCCGGAAACAGGGTTCCCGAttcgcga                                                                                                                                                                              >  2:268126/17‑239 (MQ=255)                                                                                   aCGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAGCCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAg                                                                                                                                                                 <  1:487400/251‑1 (MQ=255)                                                                                      gTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCACGATGCGAGGCGATCTCGCCAAgcgc                                                                                                                                                              >  1:148283/1‑251 (MQ=255)                                                                                          ccATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGAGCTCGCCAAGCGCGAAc                                                                                                                                                          <  1:304538/251‑1 (MQ=255)                                                                                          ccATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCATGTTATGAAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCCAGCGCGAAc                                                                                                                                                          >  1:299647/1‑251 (MQ=255)                                                                                            atatACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTAGCCAAGCGCGAAccg                                                                                                                                                        >  1:158544/1‑250 (MQ=255)                                                                                                 cAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAAt                                                                                                                                                   <  1:1494/251‑1 (MQ=255)                                                                                                  aaGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATg                                                                                                                                                  >  2:362547/1‑251 (MQ=255)                                                                                                       gtGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGgc                                                                                                                                             >  1:288468/1‑251 (MQ=255)                                                                                                                         cGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTCAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatgatg                                                                                                                            >  1:153840/1‑250 (MQ=255)                                                                                                                          gCAAGGAGCAGCGATTTGCGTCGCCGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCTAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCGCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTgatgatgat                                                                                                                          <  2:153840/251‑1 (MQ=255)                                                                                                                                 gcagcGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTCGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACg                                                                                                                   <  1:453002/251‑1 (MQ=255)                                                                                                                                     cGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGGATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGcat                                                                                                               <  1:395863/251‑1 (MQ=255)                                                                                                                                     cGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGcat                                                                                                               >  1:324258/1‑251 (MQ=255)                                                                                                                                           gCGTCGCTGGACGACCTGAAAGCGCAGATGGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTTACAAAACCGGCTTAAGCCTGTGATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGTGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGATTGATGATGATCTGTACGGCATCATCCg                                                                                                         <  2:241268/251‑1 (MQ=255)                                                                                                                                              gcGCTGGACGAACTGAAAGCGCAGATTTCGTGTGAGGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGTGAATCTGATGAGTGACTATAAAGCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCATGc                                                                                                      <  1:24917/250‑1 (MQ=255)                                                                                                                                                           tGAAAGCGCAGATTTCGCGTGATTAATTAACCGCCCGCGAAGTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGTAATGCTGGCTCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAgg                                                                                          <  2:439048/250‑1 (MQ=255)                                                                                                                                                            gAAAGCTCAGATTGCTCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGTAACCGAGAATCTGATGAGTGACTATAAATCAAACCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGTGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGTTAAAAAAGGc                                                                                         <  1:269540/250‑1 (MQ=255)                                                                                                                                                                 cgcAGCTTGCGCGTGATGAATTAACCGCCCGCGATTTTTTTGGGCTAACAAAACCGGCTTCAGCCTGTTATGAAATCAAACCGAAACACGGAACCGCGAATCTGATGAGTGACTATAAATCAACCCTGACTTTGCCGGAACAAGGGTTCCCGATGCTGGGCGATCTCGCAAAGCGCGAACCGGGAATGCTGGCACGTTGGACTGATGGTGATCTGTACGGCATCATCAGAGCGACTAAAAAAGACaaaaa                                                                                    >  2:18707/1‑250 (MQ=255)                                                                                                                                                                     gATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACtgat                                                                                                                                <  1:61100/202‑1 (MQ=255)                                                                                                                                                                     gATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACtgat                                                                                                                                >  2:61100/1‑202 (MQ=255)                                                                                                                                                                     gATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGACTCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCt                                                                               >  1:335353/1‑251 (MQ=255)                                                                                                                                                                                                          gggCTAACAAAACCGGTGTAATCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCGTATGAGTGACTATAATTCAACCCTGAATTTGCCGTAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAAGGCTGGCGCGTTGGACTGATGATTATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCGGCATGATGGCCCTCCTTATTCGAATGgcag                                          <  2:453045/251‑1 (MQ=255)                                                                                                                                                                                                                   aaaCCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCatat                                 >  1:231977/1‑251 (MQ=255)                                                                                                                                                                                                                        ggCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTc                            >  1:361843/1‑251 (MQ=255)                                                                                                                                                                                                                        ggCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTc                            >  1:160819/1‑251 (MQ=255)                                                                                                                                                                                                                              aGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCgg                      <  1:298324/251‑1 (MQ=255)                                                                                                                                                                                                                                                   gAAACACGGAACCGCGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGTGTTCACGATGCGTGGCGATCTCGCCAAGCGCGCACCGGGAATGCTGGCGCGATGGACTGATGTTCATCTGGACGGCATCATCCGTGCGGCTAAAACAGGCAAAACCACCTTCTTTCTGCATGTTGGCCCTCCTTATGCGAGTGGCAGCATTACTCTTGGTCACTCGATTAAAAATCTTCCGAAAGAc  >  2:65444/1‑250 (MQ=255)                                                                                                                                                                                                                                                   gAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGt                             <  2:180992/223‑1 (MQ=255)                                                                                                                                                                                                                                                   gAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGt                             >  1:180992/1‑223 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      GCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTTAACAAGATTCTGAAAGAC  >  NC_000913/22127‑22618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑