Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 21,750 A→T H115L (CAT→CTT)  ribF → bifunctional riboflavin kinase/FMN adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091321,7500AT98.3% 183.5 / NA 59H115L (CAT→CTT) ribFbifunctional riboflavin kinase/FMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (25/33);  minor base G (1/0);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCAAGACTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACAATCTGGCTCTGGCAGAGAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTA  >  NC_000913/21503‑21991
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
cgcgcgCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCtttt                                                                                                                                                                                                                                                 <  1:117699/251‑1 (MQ=255)
              gggCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTc                                                                                                                                                                                                                                   >  1:322975/1‑251 (MQ=255)
                      aGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGACACACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTACAATTTCTTGCCGt                                                                                                                                                                                                                            >  2:175278/1‑250 (MQ=255)
                               ggCGCAAGCTCAACTTTCCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGTCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGGgatgat                                                                                                                                                                                                                  <  2:91337/251‑1 (MQ=255)
                                 cgcaagcgcaaCTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGAttt                                                                                                                                                                                                                <  1:427582/251‑1 (MQ=255)
                                  gcaagcgcaaCTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCATGCCGTAGGTGATGATTTc                                                                                                                                                                                                               >  1:107409/1‑251 (MQ=255)
                                              tACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCt                                                                                                                                                                                                   >  1:78907/1‑251 (MQ=255)
                                                   gtgatggtgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCg                                                                                                                                                                                              <  1:371990/251‑1 (MQ=255)
                                                     gatggtgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAAtt                                                                                                                                                                                                                                     >  1:232710/1‑210 (MQ=255)
                                                     gatggtgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAAtt                                                                                                                                                                                                                                     <  2:232710/210‑1 (MQ=255)
                                                        ggtgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAg                                                                                                                                                                                         <  1:327580/251‑1 (MQ=255)
                                                          ggatgCTTTGTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGGGAAGGc                                                                                                                                                                                       <  2:481300/250‑1 (MQ=255)
                                                            atgCTTTTTGAACCTAAACCACTGGAACTGTTTGCTACCGATAAAGCCCCTGCACGGCTGACCCTGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGAGGTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCAGAGTTAACCGCGCAAAATTTAATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGAGATGATTTCCGCTTTGGCGCTGGTAGTGAAGGCgg                                                                                                                                                                                     >  2:263599/1‑250 (MQ=255)
                                                              gCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGAtt                                                                                                                                                                                   <  1:48935/251‑1 (MQ=255)
                                                                  tttGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑Ct                                                                                                                                                                               <  1:288927/250‑1 (MQ=255)
                                                                    tGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTg                                                                                                                                                                             <  1:17677/250‑1 (MQ=255)
                                                                          ccAACCACTGGAACGGTTTGCTTCCGATAAAGCCCCGGGACGGCTGACCCGGCTGCTGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGGCGTGAAGGCGATTT‑CTTGTTATTAc                                                                                                                                                                      <  2:391897/250‑1 (MQ=255)
                                                                            aaCCACTGGACCTGTTTGCTACCGATAAAGCCCCTGCACGGCTGACCCGGCTGCGGGAAAACCTGCGTTACCTTGCCGAGTGCGGCGTTGATTACGAGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGCTGATTTCCGCTTTGGCGCTGGTCGTGAATGCGATTT‑CTTGTTATTAc                                                                                                                                                                      >  2:487400/1‑249 (MQ=255)
                                                                            aaCCACTGGAACTGTTTGCTACCGAGAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAg                                                                                                                                                                    <  2:245880/251‑1 (MQ=255)
                                                                              ccACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGaa                                                                                                                                                                  <  2:291374/251‑1 (MQ=255)
                                                                              ccACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGa                                                                                                                                                                   >  1:233055/1‑250 (MQ=255)
                                                                                   ggAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTg                                                                                                                                                             <  1:368832/251‑1 (MQ=255)
                                                                                   ggAACTGTTTGCTACCGATAAAGCCCCGGCACGGCGGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTGTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATGTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTg                                                                                                                                                             <  2:247138/251‑1 (MQ=255)
                                                                                      aCTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGc                                                                                                                                                           >  1:367974/1‑250 (MQ=255)
                                                                                          tttGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACTGAAAGCTGGCATgg                                                                                                                                                       >  1:83272/1‑250 (MQ=255)
                                                                                            tGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGaa                                                                                                                                                     <  2:26473/250‑1 (MQ=255)
                                                                                               tACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACg                                                                                                                                                 >  1:442606/1‑251 (MQ=255)
                                                                                                aCCGATAAAGCCCCTGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGATTTTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTTAAGCTTTTGCGCGTAAAATTTCTTTCCGTAGGTGATGATTTCCGCTTTTGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACg                                                                                                                                                 <  1:51399/250‑1 (MQ=255)
                                                                                                             cggcacggcTGACCCGGCTGCGGGAAAAACTGCGTTATCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGgcg                                                                                                                                                                                                     <  1:343244/178‑1 (MQ=255)
                                                                                                             cggcacggcTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGCGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGgcg                                                                                                                                                                                                     >  2:343244/9‑186 (MQ=255)
                                                                                                             cggcacggcTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCAcc                                                                                                                                   <  2:129437/243‑1 (MQ=255)
                                                                                                                        aCCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGAGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGAATGGCATACGGCTTCGAAATCACCAGTACGCaaa                                                                                                                         >  2:453134/1‑250 (MQ=255)
                                                                                                                            ggCTGTGGGAAAAACTGCGTTGCCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACtttt                                                                                                                    <  2:83798/251‑1 (MQ=255)
                                                                                                                                gCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCg                                                                                                                >  2:108027/1‑251 (MQ=255)
                                                                                                                                 cGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCgg                                                                                                               >  1:128608/1‑250 (MQ=255)
                                                                                                                                   ggAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGa                                                                                                               >  1:439048/1‑249 (MQ=255)
                                                                                                                                      aaaaCTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGCTTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTg                                                                                                          >  2:494205/1‑251 (MQ=255)
                                                                                                                                            gCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTg                                                                                                     <  1:408908/250‑1 (MQ=255)
                                                                                                                                                 aCCTTGCAGAGAGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGCAGCTTTTGCGCGTAAAATTTCTTGCCGTCGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATc                                                                                               >  2:38793/1‑251 (MQ=255)
                                                                                                                                                           gggggtcGTTTATTACGTGTTGTGTGGGCGTTTCTGCAGTAGTGTCGAGGCGTTAACCGCGCAAGAGTGCATCAGCGAACGTCTGGTGAGGCTTTTGCGCGTAAAAGTTCTTGGCGTAGATGATGATTTCCGATTTGTCGCTGGTTGTGAAGGCGATTT‑CTTGTGATTACAGAAAGCTGGCAGGGAATTCGGCTTCGATATCACCAGTACGCAAACTTTTTGCTAAGGTGGCGTGCGTATCAGCAGCAccg                                                                                     <  2:409093/245‑1 (MQ=255)
                                                                                                                                                                 cGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGc                                                                               <  1:115542/251‑1 (MQ=255)
                                                                                                                                                                  gTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCg                                                                              >  1:80576/1‑251 (MQ=255)
                                                                                                                                                                         acgggCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAgg                                                                         <  2:447914/246‑1 (MQ=255)
                                                                                                                                                                          cGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGAGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGcgc                                                                      <  2:361283/251‑3 (MQ=255)
                                                                                                                                                                             gCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGcgctg                                                                    <  1:8580/250‑5 (MQ=255)
                                                                                                                                                                              cTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGACGGTGGCGTGCGTATCAGCCGCACCGATGTGCGTCAGGcgctggg                                                                  >  2:100121/1‑245 (MQ=255)
                                                                                                                                                                                  gcgtgcgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGGGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCgg                                                                >  2:225162/1‑249 (MQ=255)
                                                                                                                                                                                  gcgtgcgtTTCGACAGGCGTTTCGCGGCGGTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGAt                                                              <  1:459765/251‑1 (MQ=255)
                                                                                                                                                                                        gtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCGTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACTAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGACAGGCGCTGGCGGGTGAccat                                                        >  2:395036/1‑248 (MQ=255)
                                                                                                                                                                                           tCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtct                                                      <  1:265283/250‑1 (MQ=255)
                                                                                                                                                                                             gACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtctggc                                                   >  1:146883/1‑251 (MQ=255)
                                                                                                                                                                                                             cgTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGtttt                                   >  2:160813/1‑250 (MQ=255)
                                                                                                                                                                                                                tAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACt                                 <  1:38793/250‑1 (MQ=255)
                                                                                                                                                                                                                 aaCCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGATTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCCGGCGCTGGCGGATGACAATCCTGCTCTGGCAGAAAGTTTACTgg                               >  2:298324/1‑251 (MQ=255)
                                                                                                                                                                                                                        cAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCAccc                        <  1:494205/251‑1 (MQ=255)
                                                                                                                                                                                                                         aaaaTTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGAGGACAATCTGGCCCTGGCAGAGAGTTTACTGGGGCACCCg                       >  1:368859/1‑251 (MQ=255)
                                                                                                                                                                                                                           aaTTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTGCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtctggctct                                                <  1:319370/224‑1 (MQ=255)
                                                                                                                                                                                                                                    aGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCAtctc            <  1:52856/251‑1 (MQ=255)
                                                                                                                                                                                                                                              gggTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTGTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCTGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCTTTTGCCATCTCCGGGCGTGTa  <  2:30125/250‑1 (MQ=255)
                                                                                                                                                                                                                                               ggTGAAGCTTTTGCGCGTAAAATTTCTTGTCGTAGGTGATGATTTCCGCTTGGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCTCCCGTTTGCCATCTCCGGGCGTGTa  <  2:78907/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
CGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCAAGACTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTT‑CTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACAATCTGGCTCTGGCAGAGAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTA  >  NC_000913/21503‑21991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑