Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	20,590	T→C	intergenic (‑82/+225)	insA1 ← / ← rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	20,590	0	T	C	100.0%	20.9 / NA	8	intergenic (‑82/+225)	insA1/rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/2);  total (6/2)

TCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCT  >  NC_000913/20542‑20834                                                 |                                                                                                                                                                                                                                                     aatagagtggtcttaagtttaatacctcaatgtgtatcacaatatccacATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgc                                           >  1:61710‑M1/23‑161 (MQ=255)   tagagtggtcttaagtttaatacctcactgtgtatcacaatatccACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgc                                         >  2:274043‑M1/21‑159 (MQ=255)                 gtttaatacctcaatgtGTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGCGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcaca                            >  1:43009‑M1/7‑145 (MQ=255)                  tttaatacctcaatgTGTATCTCAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGATTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgcctatctggca                                                                                                      <  2:263753‑M1/170‑32 (MQ=255)                  tttaatacctcaatgTGTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggca                                                                                                      >  1:263753‑M1/6‑144 (MQ=255)                                 tgtATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTTCGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTCAGCCACGAAGAATTCa                                                                                                                                                         >  2:297790/1‑109 (MQ=255)                                 tgtATCACAATATCAACAGTCTTGGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCa                                                                                                                                                         <  1:297790/109‑1 (MQ=255)                                        caATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgatta     >  2:439508‑M1/1‑122 (MQ=255)                                          atatCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagc  >  1:77048‑M1/1‑120 (MQ=255)                                                 |                                                                                                                                                                                                                                                     TCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCT  >  NC_000913/20542‑20834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑