Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,627	G→C	intergenic (+7/+184)	nhaR → / ← insB1	DNA‑binding transcriptional activator NhaR/IS1 family protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,627	0	G	C	100.0%	82.7 / NA	29	intergenic (+7/+184)	nhaR/insB1	DNA‑binding transcriptional activator NhaR/IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/13);  total (16/13)

CCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGAT  >  NC_000913/19394‑19818                                                                                                                                                                                                                                          |                                                                                                                                                                                                 ccGCGTTGATGAAAGCTTTTGGTGCGATGAACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACCGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTCATCGGCACCTCCCCCCAAAGTTa                                                                                                                                                                                   >  1:361610/1‑249 (MQ=255)        gatgaAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCCCCTCCCCCCAAAGTTAAGGTgggg                                                                                                                                                                           <  2:503433/250‑1 (MQ=255)                         cGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTAc                                                                                                                                                         <  1:172490/251‑1 (MQ=255)                               acaATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCCGCGGCGCGTTAATCGGCACCTCCCCCTAAAGTTAAGGGGGGGGAGATAGATTAGTTGTACATAAcc                                                                                                                                                   >  2:380519/1‑251 (MQ=255)                                    gCAATCTTCGTTGTCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGAt                                                                                                                                              <  2:293434/251‑1 (MQ=255)                                                             aTGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGCATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCg                                                                                                                     >  1:235019/1‑251 (MQ=255)                                                                cATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGTTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCtt                                                                                                                  <  1:32090/251‑1 (MQ=255)                                                                  tatGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGAACCCGGCGGTACAGCGAATCTGCACTACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGAGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGAATAGGCTCATTATTTGCCCGCTTg                                                                                                                 >  1:471884/1‑250 (MQ=255)                                                                   atGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTga                                                                                                                <  2:386152/250‑1 (MQ=255)                                                                     gACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATACCCACGATTTTGACTCGGCTCATTATTTGCCCGCTTggga                                                                                                              >  2:106787/1‑247 (MQ=255)                                                                        ttttATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACAtt                                                                                                          >  1:205110/1‑251 (MQ=255)                                                                              gCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGCATGTGATGGAAGAGAACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTAGGCTCATTATTTGCCCGCTTGAGACATTATTTcc                                                                                                    >  2:495674/1‑251 (MQ=255)                                                                                       aCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAcg                                                                                           <  1:249128/251‑1 (MQ=255)                                                                                            cGTAGAAATTGGTCGCGTCGAGAATTTGATGGAAGAGTACCATGCTATTTATGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGGCCGGCGACGCGTTAATCGGCACCTCCCCCCAAAGTGAAGATGGGGGAGATAGATTAGTTGTACATAACCGCGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCggg                                                                                       <  1:60565/250‑1 (MQ=255)                                                                                                     tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGaataaat                                                                              <  2:33953/250‑1 (MQ=255)                                                                                                                agaATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGGCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGa                                                                  <  2:189207/251‑1 (MQ=255)                                                                                                                               agTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATCCGGATTATTCTGCGCTTTTTTGTCCAGCGGCGCGTTAATAGGCACCTCCCCCCAAAGTTAAGGGGGGGGAGATAGATTAGTTGTACATAACCCCGATTTTGACTCGGCTCCTTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAggc                                                                        >  1:205675/1‑229 (MQ=255)                                                                                                                               agTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCCCTTGAGACATTATTTCCATATGTACGCGGGCGACTAAATAGTGGAATATGATTACTTCCTTCATgg                                                   >  2:275173/1‑251 (MQ=255)                                                                                                                               agTACCATGCTATTGTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAACCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAAGAGAGGa                                                                        <  2:205675/230‑1 (MQ=255)                                                                                                                                   ccATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGAt                                               <  1:430037/251‑1 (MQ=255)                                                                                                                                     aTGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGc                                             >  2:5587/1‑251 (MQ=255)                                                                                                                                         tATTTTTGCTGAGCGGATGATTCAGCACACGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGCATCTGATTACTTCCTTCATGGGGATGCTGa                                          >  1:400985/1‑250 (MQ=255)                                                                                                                                          aTTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGaa                                         >  1:479570/1‑250 (MQ=255)                                                                                                                                           tttttGCTGAGCGGATGATTCAGCACCCGGCGATACAGCGAATCTGCAATACGGATTATTCTTCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGGGGGGGAGAATGCTTAGTTGTACATACCCACGATTTTGACTCTGCTCATTATTTGCCCGCTTGAGCCATTATTTCAATTTGTACGCGGGCGCATAACTAGAGTCATCTGATTTCTTCCTTCATGGGGATGCTGaca                                        >  2:76182/1‑248 (MQ=255)                                                                                                                                                gCTGAGCGGTTGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGGCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGagta                                  <  2:479570/251‑1 (MQ=255)                                                                                                                                                 cTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                            <  2:377020/192‑1 (MQ=255)                                                                                                                                                 cTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                            >  1:377020/1‑192 (MQ=255)                                                                                                                                                         atgatTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGAATCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTATGCGGGCGAATAAATAGAGGAATCTGTTTACTTCCTTCATGGGGATGCTGACAAGAGTAGTAATTGCt                         >  2:273703/1‑251 (MQ=255)                                                                                                                                                                                gCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttcattcg  >  2:143793/1‑228 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                 CCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGAT  >  NC_000913/19394‑19818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑