Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 19,613 T→C V300A (GTG→GCG)  nhaR → DNA‑binding transcriptional activator NhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091319,6130TC97.1% 99.1 / ‑4.1 34V300A (GTG→GCG) nhaRDNA‑binding transcriptional activator NhaR
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (17/16);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

AGAAATCCTCGGCGAGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGAT  >  NC_000913/19368‑19818
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                              
aGAAATCCTCGGCGAGGTTGATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGt                                                                                                                                                                                                            <  2:124204/250‑1 (MQ=255)
         cGGCGAGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTGTGCCGATAAAACTGTTGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCa                                                                                                                                                                                                   <  1:136513/250‑1 (MQ=255)
         cGGCGAGGTTGAGTATGCCGTGTTGATGAAAGCTTTTGGTTCGATGCACAATTCAATCTTCGGTGCCCAAACTCTTTATGCATATGATTTTTATGCCGATAAAACTGTCGGAGAAATTGTTCTCGTCGAGAATGTGATGGTAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCTCCCGGCGGTACAGCTAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAACCGGCa                                                                                                                                                                                                   <  2:486569/250‑1 (MQ=255)
                          ccGCGTTGATGAAAGCTTTTGGTGCGATGAACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACCGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTCATCGGCACCTCCCCCCAAAGTTa                                                                                                                                                                                   >  1:361610/1‑249 (MQ=255)
                                 gatgaAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCCCCTCCCCCCAAAGTTAAGGTgggg                                                                                                                                                                           <  2:503433/250‑1 (MQ=255)
                                                  cGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTAc                                                                                                                                                         <  1:172490/251‑1 (MQ=255)
                                                        acaATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCCGCGGCGCGTTAATCGGCACCTCCCCCTAAAGTTAAGGGGGGGGAGATAGATTAGTTGTACATAAcc                                                                                                                                                   >  2:380519/1‑251 (MQ=255)
                                                             gCAATCTTCGTTGTCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGAt                                                                                                                                              <  2:293434/251‑1 (MQ=255)
                                                                                      aTGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGCATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCg                                                                                                                     >  1:235019/1‑251 (MQ=255)
                                                                                         cATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGTTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCtt                                                                                                                  <  1:32090/251‑1 (MQ=255)
                                                                                           tatGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGAACCCGGCGGTACAGCGAATCTGCACTACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGAGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGAATAGGCTCATTATTTGCCCGCTTg                                                                                                                 >  1:471884/1‑250 (MQ=255)
                                                                                            atGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTga                                                                                                                <  2:386152/250‑1 (MQ=255)
                                                                                              gACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATACCCACGATTTTGACTCGGCTCATTATTTGCCCGCTTggga                                                                                                              >  2:106787/1‑247 (MQ=255)
                                                                                                 ttttATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACAtt                                                                                                          >  1:205110/1‑251 (MQ=255)
                                                                                                       gCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGCATGTGATGGAAGAGAACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTAGGCTCATTATTTGCCCGCTTGAGACATTATTTcc                                                                                                    >  2:495674/1‑251 (MQ=255)
                                                                                                          gATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATc                                                                                                                                                                                                       <  1:304739/149‑1 (MQ=255)
                                                                                                          gATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATc                                                                                                                                                                                                       >  2:304739/1‑149 (MQ=255)
                                                                                                                aCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAcg                                                                                           <  1:249128/251‑1 (MQ=255)
                                                                                                                     cGTAGAAATTGGTCGCGTCGAGAATTTGATGGAAGAGTACCATGCTATTTATGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGGCCGGCGACGCGTTAATCGGCACCTCCCCCCAAAGTGAAGATGGGGGAGATAGATTAGTTGTACATAACCGCGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCggg                                                                                       <  1:60565/250‑1 (MQ=255)
                                                                                                                              tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGaataaat                                                                              <  2:33953/250‑1 (MQ=255)
                                                                                                                                         agaATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGGCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGa                                                                  <  2:189207/251‑1 (MQ=255)
                                                                                                                                                        agTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATCCGGATTATTCTGCGCTTTTTTGTCCAGCGGCGCGTTAATAGGCACCTCCCCCCAAAGTTAAGGGGGGGGAGATAGATTAGTTGTACATAACCCCGATTTTGACTCGGCTCCTTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAggc                                                                        >  1:205675/1‑229 (MQ=255)
                                                                                                                                                        agTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCCCTTGAGACATTATTTCCATATGTACGCGGGCGACTAAATAGTGGAATATGATTACTTCCTTCATgg                                                   >  2:275173/1‑251 (MQ=255)
                                                                                                                                                        agTACCATGCTATTGTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAACCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAAGAGAGGa                                                                        <  2:205675/230‑1 (MQ=255)
                                                                                                                                                            ccATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGAt                                               <  1:430037/251‑1 (MQ=255)
                                                                                                                                                              aTGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGc                                             >  2:5587/1‑251 (MQ=255)
                                                                                                                                                                  tATTTTTGCTGAGCGGATGATTCAGCACACGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGCATCTGATTACTTCCTTCATGGGGATGCTGa                                          >  1:400985/1‑250 (MQ=255)
                                                                                                                                                                   aTTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGaa                                         >  1:479570/1‑250 (MQ=255)
                                                                                                                                                                    tttttGCTGAGCGGATGATTCAGCACCCGGCGATACAGCGAATCTGCAATACGGATTATTCTTCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGGGGGGGAGAATGCTTAGTTGTACATACCCACGATTTTGACTCTGCTCATTATTTGCCCGCTTGAGCCATTATTTCAATTTGTACGCGGGCGCATAACTAGAGTCATCTGATTTCTTCCTTCATGGGGATGCTGaca                                        >  2:76182/1‑248 (MQ=255)
                                                                                                                                                                         gCTGAGCGGTTGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGGCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGagta                                  <  2:479570/251‑1 (MQ=255)
                                                                                                                                                                          cTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                            <  2:377020/192‑1 (MQ=255)
                                                                                                                                                                          cTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                            >  1:377020/1‑192 (MQ=255)
                                                                                                                                                                                  atgatTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGAATCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTATGCGGGCGAATAAATAGAGGAATCTGTTTACTTCCTTCATGGGGATGCTGACAAGAGTAGTAATTGCt                         >  2:273703/1‑251 (MQ=255)
                                                                                                                                                                                                         gCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttcattcg  >  2:143793/1‑228 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                              
AGAAATCCTCGGCGAGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGAT  >  NC_000913/19368‑19818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: