Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,893	T→C	A135A (GCT→GCC)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,893	0	T	C	100.0%	185.0 / NA	55	A135A (GCT→GCC)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/26);  total (29/26)

AACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTG‑CCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGG  >  NC_000913/17660‑18136                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    aaCATGCTGTTATGGATAAAGTACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGgtgtgct                                                                                                                                                                                                                                    <  2:217999/251‑4 (MQ=255)     ggCTGTTATGGATAAATGACGCGCTGATGGCGGGATTTTTCCTGTTTGTCGGTCTGGAAGTTAAACGTGAGCTGATGCCAGGATCGCTAGCCAGCTTAAGCCAGGCCGCATTTCCAGTTATCGCCGATATTTGTGGGGTGATT‑TGTCCGGCCTTACTCTATATGGCCTTTAACTATGCCGAACCGCTTACCCGCGACGGGGGGGCGGTCCCTGCGGCTACTTACATTGCCTTTGCACtcgggtggctggcg                                                                                                                                                                                                                                >  2:241389/2‑238 (MQ=255)      gCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTAGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCCGCTTACGCCAGGCCGCATTTCCAGTTTTCGCCGCTATTGGTGGGATGATTGTT‑TCGGCATTACTCTATCTGGCCTTTAAATATGCCGATCCGATTCACCGCGCAGGGTGGGCGATCCCGGCGGCTTCTGACCTTGCCGTTGCACTTGGTGGTCTGgcgc                                                                                                                                                                                                                               >  2:356597/1‑251 (MQ=255)              gtatacatgacttgcgtaTGGCGGTATTTTTCCTGTTAGTCGGTCGGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGTCGCATTTCCATTGCTCGCCGCTATTTGTGGTATGATTGTT‑CCGGCATTACTCGATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGATGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTGTGCACTTGGTGTTCTGGCGCTGTTggg                                                                                                                                                                                                                        <  2:217932/233‑1 (MQ=255)                  aaaTGACGCGCTGATGGCGGGATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGCTGCAAGGATCGCTAGGCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCAATACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGGCCTTGCCTTTGCCCTTGGTGGTGTGGCGCTGTTGGGAAGtg                                                                                                                                                                                                                   >  2:401710/1‑250 (MQ=255)                                 ggCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAgcg                                                                                                                                                                                                     <  2:103665/250‑1 (MQ=255)                                    ggTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACCTTGCCTTTGCACTTGGTGGGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTg                                                                                                                                                                                                  >  2:238529/1‑250 (MQ=255)                                          tttCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATc                                                                                                                                                                                            <  1:397388/250‑1 (MQ=255)                                             ccTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCtttt                                                                                                                                                                                        >  1:26166/1‑251 (MQ=255)                                                   aGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATgg                                                                                                                                                                                  >  1:367871/1‑251 (MQ=255)                                                       gggcTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGctct                                                                                                                                                                              <  1:278499/248‑1 (MQ=255)                                                           tGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCt                                                                                                                                                                          >  1:471954/1‑251 (MQ=255)                                                               aGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCtat                                                                                                                                                                        <  1:236631/249‑1 (MQ=255)                                                               aGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCtat                                                                                                                                                                        >  2:236631/1‑249 (MQ=255)                                                               aGTTAAAAGTGAACTGAGGTAAGGATCGCTAGGAAGCATACGACAGGCCGCATTCACAGTTATCGCCGCTATGGGTGGGATGATTGTT‑CCGCCATTACTCTATCTGTCCTTTAACTAAGCCGATCCGATTACCCGCGAAGGGTGGGCGAGCCCGGCGGATACGGACATTGTCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGGTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCtatga                                                                                                                                                                      <  2:497/251‑3 (MQ=255)                                                                   aaaCGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATcg                                                                                                                                                                   >  1:167830/1‑250 (MQ=255)                                                                     aCGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATcgacg                                                                                                                                                                <  1:426814/251‑1 (MQ=255)                                                                          aaatgctGCACGGATCGGTACCCCGCTTACGCCAGCCCGAATTTCCCGTTATCGCCGCCCTTGGTGGGATGATTGTT‑CCGCAATTAATCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCCCCTGGCGGGCTGGCGCTGTGGCGCAGCCGTGTTCCGCCAGAGCAGAAGATCTTTTTGCCGTCTCTGGATATCATCGACGATCtt                                                                                                                                                           >  2:458593/7‑251 (MQ=255)                                                                            cTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTgg                                                                                                                                                         >  2:287907/1‑251 (MQ=255)                                                                                   aaGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCcatc                                                                                                                                                  <  1:124689/251‑1 (MQ=255)                                                                                     ggATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGCTGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGCTCTTGGGGCcatcat                                                                                                                                                >  2:207181/1‑251 (MQ=255)                                                                                       aTCGCTAGCCAGCTTACGCCAGGCCGCATTGCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTTGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTa                                                                                                                                              <  1:91218/251‑1 (MQ=255)                                                                                             aGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCg                                                                                                                                        >  1:151093/1‑251 (MQ=255)                                                                                              gccagttttagcccggCCGCATTTCCAGTTATCGCCGCTATGGGTGGGAGGATGTTG‑CCGGCATTCCTTTATCCGGTCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTTCTTGCATTGCCTTTGCTCTTGGTGTGCTGGCGCTTTTGGGTAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATTGCTCTGGCTATTACCGACGATCTTGGGGCCATCATTatcaccg                                                                                                                                        <  2:505923/236‑4 (MQ=255)                                                                                                cAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCAt                                                                                                                                     >  1:52879/1‑251 (MQ=255)                                                                                                  gCTTCCGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTg                                                                                                                                   <  1:378945/251‑1 (MQ=255)                                                                                                      aCGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGAGTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGAGGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCt                                                                                                                               <  1:236474/251‑1 (MQ=255)                                                                                                          cAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTacac                                                                                                                           <  1:393604/251‑1 (MQ=255)                                                                                                                   tttCCAGTTATCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGTTCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATAACtt                                                                                                                  >  2:85715/1‑251 (MQ=255)                                                                                                                         gtgaTCGCCGCTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGCAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGTTGGCTCTGGCTATTATCGACGATCTTGGGGACATCATTATCATCGCATTGTTCTTCACTAATGCCTTATCggt                                                                                                            >  2:92327/4‑249 (MQ=255)                                                                                                                          ttATCGCCGCTATTGGTGGGCTGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATg                                                                                                           >  2:185467/1‑251 (MQ=255)                                                                                                                              cgccgcTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGCTCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTCTACTAATGACTTATCGATGGCct                                                                                                       >  2:268226/1‑251 (MQ=255)                                                                                                                               gcagcTATTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGGTTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGGTGGCTCTGGCTATTATCGACGTTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACCTATCGATGGCctc                                                                                                      >  2:812/4‑251 (MQ=255)                                                                                                                                     aTTGGTGGGATGATTGTT‑CCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCtt                                                                                                  <  1:256397/249‑1 (MQ=255)                                                                                                                                                     gttccGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAg                                                                                  <  1:238529/247‑1 (MQ=255)                                                                                                                                                       tccGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGCTTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGAAGGCCTCTCTTGGCGTCGCGGCTGTAGCa                                                                                >  2:406467/2‑250 (MQ=255)                                                                                                                                                                aCTCTATTCGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTa                                                                       <  2:293822/250‑1 (MQ=255)                                                                                                                                                                aCTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTAGCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTAc                                                                      <  1:444916/251‑1 (MQ=255)                                                                                                                                                                        tGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTCCTCGCGGt                                                               >  2:414017/1‑250 (MQ=255)                                                                                                                                                                              ttAACTATGCCGATCCGATTACCCGCGAAGGGAGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGTTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGGATTGAAt                                                        >  1:98053/1‑251 (MQ=255)                                                                                                                                                                                aaCTATGACGATCCGATTACCCGCGAAGGGTGGGCGCTCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTATTGGCGTCGAGGCTGTAGCTATAGCGGTACTccagggttttgatat                                                      >  2:264479/1‑237 (MQ=255)                                                                                                                                                                                    aTGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCtgtgt                                                  >  1:234406/1‑251 (MQ=255)                                                                                                                                                                                      gCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACCAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCtgtgtg                                                 <  1:12789/250‑1 (MQ=255)                                                                                                                                                                                              gatTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTACCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTAcgcc                                        >  1:487514/1‑251 (MQ=255)                                                                                                                                                                                                  aCCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTGGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCa                                     <  1:63229/250‑1 (MQ=255)                                                                                                                                                                                                         aaGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTc                             <  1:264562/251‑1 (MQ=255)                                                                                                                                                                                                              ggggCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTatat                        <  1:344266/250‑1 (MQ=255)                                                                                                                                                                                                                ggCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTc                      >  1:37044/1‑251 (MQ=255)                                                                                                                                                                                                                       ccGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTgg               >  1:204841/1‑251 (MQ=255)                                                                                                                                                                                                                                attGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAgtgt       <  1:85715/248‑1 (MQ=255)                                                                                                                                                                                                                                   gACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGtgttg     <  1:207181/249‑1 (MQ=255)                                                                                                                                                                                                                                    aCATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTgg  >  1:322053/1‑251 (MQ=255)                                                                                                                                                                                                                                    aCATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGGACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTgg  <  2:52879/251‑1 (MQ=255)                                                                                                                                                                                                                                     cATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTcg                                                                   <  2:34798/185‑1 (MQ=255)                                                                                                                                                                                                                                     cATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTcg                                                                   >  1:34798/1‑185 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    AACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTG‑CCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGG  >  NC_000913/17660‑18136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑