Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 17,383 A→G intergenic (+373/‑106) sokC → / → nhaA small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091317,3830AG95.3% 118.4 / ‑3.2 43intergenic (+373/‑106)sokC/nhaAsmall regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (21/20);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

ATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATG‑ATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑A‑C‑GCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGC  >  NC_000913/17142‑17630
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
aTGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTATAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGATGATGGCGCACATTATTCAATGGATCGTa                                                                                                                                                                                                                                                  >  2:397388/1‑250 (MQ=255)
           attTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTTGATTATTTTTGAAATATTGTCCAAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCGTAAGCTATG‑ATTATCGGGGCTTAGGGTCACTCGTGAGCGCTGTCAGCCGTC‑AA‑AA‑AAC‑ACATCTCTCCGCGGATGGCGGAAATTCTTCAATGGATCGTAAAAAACGagtt                                                                                                                                                                                                                                       <  2:308009/250‑4 (MQ=255)
           attTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAACCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGagtt                                                                                                                                                                                                                                       <  2:151532/250‑4 (MQ=255)
                aCTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTACTTAATCCCTCAGAAATCCTCACCTTAAGCTATA‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AACA‑C‑TAATATCACCGCTGATGGCACAAATTCTTCAATGGATCGTAAAAAACgtgtaattcct                                                                                                                                                                                                                                 >  2:278499/1‑241 (MQ=255)
                        ttATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGTAATCTTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGACGCAAATTCTTCAATGGCTCGTAAAAAACGAGTTATTCATACACtat                                                                                                                                                                                                                          >  2:426814/1‑250 (MQ=255)
                            ctctTTATCGCACATATTGACGACAAGCTGGATTATTTTTGAAATATTGTCATAACAAATAACGCCGCCTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCCCCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTAGTGAGCGCTTACAGCCGTC‑AA‑AA‑A‑CCTCATATCACCGCTGATGGCGCAAATTCTTCAATGGTTCGAAAACAAAGAGTTATTCCTACACTATCATCt                                                                                                                                                                                                                     >  2:378540/1‑251 (MQ=255)
                                  agcGCAAATATTGACGACAAGCTGGATTATTTGTGTAAGATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGTGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGAtgt                                                                                                                                                                                                                <  2:204937/248‑3 (MQ=255)
                                  aTCGCAATTATTGACGACAAGATGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGAtttt                                                                                                                                                                                                               >  2:323167/1‑251 (MQ=255)
                                         ttattGACGACAAGCTGGATTATTGTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGACATCCTCACATTAAGCTATG‑ATTATCTGGACTTAGGGTCACTCGTGAGCGCTTACATCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACTAGTTATTCCTACACTATAATCTGATTTTAACgatg                                                                                                                                                                                                        >  2:378945/1‑251 (MQ=255)
                                                   cAAGCTGGATTATTTTTGAAATATTGCCCTACCAATTACCGCCGCCTGCCAAGAAATTAAT‑ATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCggg                                                                                                                                                                                             <  1:223063/251‑1 (MQ=255)
                                                   aaaGCTGGATTATTTTGTAAAAATTTTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTGCCTAATTAATCCCTCAGAAATCCTCACCTTAAGC‑ATGGATTATCTGGGCTTAGGGGCACTCGTGAGCGCTTACAGTCGTC‑AA‑AA‑AAC‑GCATCTCCCCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCgg                                                                                                                                                                                              <  2:303611/250‑1 (MQ=255)
                                                        tGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGag                                                                                                                                                                                                                                         >  1:56401/1‑202 (MQ=255)
                                                        tGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAAGCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGag                                                                                                                                                                                                                                         <  2:56401/203‑2 (MQ=255)
                                                           attattTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAAt                                                                                                                                                                                      <  1:341175/251‑1 (MQ=255)
                                                            ttattTTTGAAATCTTGTCCTAACAAATAACGCCGAATGAAAAGAACTTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATA‑ATTATCTGGGATTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AACAA‑A‑C‑TCATCTCACCGCTGATGGCGCAACTTCTTCAATGGATCGTAAAAAACGTGTTATTCCTACACTATAACATGATTTTAACgatgctttattcggggaacaat                                                                                                                                                                                      >  1:365850/1‑232 (MQ=255)
                                                                 tttGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGTTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGAAAAATAGTaa                                                                                                                                                                                 >  1:293822/1‑250 (MQ=255)
                                                                   tGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATG‑ATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑A‑C‑GCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTaaaaa                                                                                                                                                                              <  1:190485/250‑1 (MQ=255)
                                                                         atTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGACACTCGTGAGCGCTTACAGCCGTC‑AA‑AATA‑C‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGATATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAGACTAGTAAACACGATCt                                                                                                                                                                        >  1:244306/1‑251 (MQ=255)
                                                                                  acaaatcatgCCGACTGACAAGAAATTACTTATTACCCTTCCTAATTAACCCCTCACAAATACTCACCTTAAGCTATG‑ATTTTCTGGGCTTAGGCTCACTAGTGACCGCTTACAGCCGTC‑AA‑AA‑A‑CATCATCTCACCGCTGACGGCGCAAATTCTTCAATGGATCGTAAAAAAAGAATTATTCCTACACTATAATCTGATTTTAACAATGATTTATGCGGGGTAAACTAGTAAAAAAGCTCTATCCACCTg                                                                                                                                                               >  2:236474/10‑251 (MQ=255)
                                                                                  aaaaataacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCAACTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTg                                                                                                                                                               <  2:427193/243‑1 (MQ=255)
                                                                                         acGCCGACTGACAAGAAAGTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAgaga                                                                                                                                                        <  1:483827/250‑1 (MQ=255)
                                                                                                     aagaaaTTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGa                                                                                                                                            <  2:37814/248‑1 (MQ=255)
                                                                                                       aaaaTTAATTATTACTTTACCTAATTAATCCCTCAGAAAACCTCACCTTAAGCTATT‑ATTATCTGGGCTTAGGGTCACTCGTGATCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCATATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCTGGGTAAAATAATAAAAACGATCTATTCCCCTGAAAGAGAAATAAAAAGTGaaa                                                                                                                                          <  2:62093/250‑1 (MQ=255)
                                                                                                              attattACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGATATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAACCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTCACGATGATTTGTGCGGGGTAAATTAGTAAAAACGATCTTTTCACCTGAAAGAGAA‑Taaaaacgacaaacatatgc                                                                                                                                  >  2:444916/1‑237 (MQ=255)
                                                                                                                    aCTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATACAAAGTGAAACATCTGCATCGAt                                                                                                                             >  1:270606/1‑251 (MQ=255)
                                                                                                                             aattaatCCCTCAAAAATCCTCACCTTAAGCTATG‑ATTAACTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCCCCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATGTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCa                                                                                                                    <  2:99928/251‑1 (MQ=255)
                                                                                                                               ttaatCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGt                                                                                                                  >  2:262957/1‑251 (MQ=255)
                                                                                                                                 aatCCCTCAGAAATCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTATTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAACTAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACCTCTGCATCGATTCTTTAGCAGTgg                                                                                                                >  2:123799/1‑250 (MQ=255)
                                                                                                                                        cagaaaTCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTc                                                                                                          <  1:406917/246‑1 (MQ=255)
                                                                                                                                            aaTCCTTACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCTTGAGCGCTTACAGCCGTA‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCTCCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAg                                                                                                     <  2:401456/251‑1 (MQ=255)
                                                                                                                                            aaTCCTCACCTTAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGCGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCggg                                                                                                       >  1:217932/1‑249 (MQ=255)
                                                                                                                                                     cctAAGCTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCGATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCGCGGGAGGCATTATTc                                                                                            <  2:302391/249‑1 (MQ=255)
                                                                                                                                                           cTATG‑ATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATc                                                                                       >  2:367861/1‑250 (MQ=255)
                                                                                                                                                                              aGGGTCACTCGTGAGCGCTTACAGCCGTCTAA‑AA‑A‑C‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCg                                                                     >  1:93143/1‑250 (MQ=255)
                                                                                                                                                                              aGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑AAC‑TCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCgg                                                                    >  1:103665/1‑250 (MQ=255)
                                                                                                                                                                                                       cggcaaa‑aa‑a‑C‑TCATCGCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAATGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAAc                                           <  1:209338/246‑1 (MQ=255)
                                                                                                                                                                                                                          ctcACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGCAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGTTTATGGCCAATAGCGGCGAAACCAGGGGATGGTATc                             >  1:437986/1‑250 (MQ=255)
                                                                                                                                                                                                                            cACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCa                            <  1:48024/249‑1 (MQ=255)
                                                                                                                                                                                                                            cACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCGTTAGCAGTGATGCCTCGGGAGGCATTATGCATATCATTGCCGCGATCCGGGCGATGATTATGGCCAATAGCGGCGCAACCAGTCGATGGTATCAc                           <  2:60793/250‑1 (MQ=255)
                                                                                                                                                                                                                               cGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGAGCGGGGTAACATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCGGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACt                       >  1:497/1‑251 (MQ=255)
                                                                                                                                                                                                                                   gATGGCGCAAATTCTTCAATGGAGCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACttt                     <  2:93143/249‑1 (MQ=255)
                                                                                                                                                                                                                                             aTTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCg         <  1:323167/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    aaTAGCTCGTAAAAAACGAATGATTCCTACACTATAATCTGATTTTAACGATGAGTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCGGAAAGAGAAGTAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCTTCGGGAGTCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGc  <  1:301432/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
ATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATG‑ATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTC‑AA‑AA‑A‑C‑GCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGC  >  NC_000913/17142‑17630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑