| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 16,966 | A→G | noncoding (15/59 nt) | sokC → | small regulatory RNA antitoxin SokC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 16,966 | 0 | A | G | 87.5% | 29.8 / ‑3.2 | 17 | noncoding (15/59 nt) | sokC | small regulatory RNA antitoxin SokC |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (5/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
GCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCA‑GCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGG‑TTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGAC‑AGCCTCAAGCACCCGCCGCTATTAT‑ATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATT > NC_000913/16720‑17147 | gcgttttcggcctccccccccGCCGTTGCACTTCCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGGCTGGCCAGTTCGGATGGGAACCTCACAGAGGTCTTTTCTCGTAACCA‑GCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGcc < 1:302872/234‑1 (MQ=255)gcgcTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCA‑GCGCCGCCACTACGGCGGGGGTCAAGATTGCGATCAGAGAGGCAAACACCCCCCTATGCTTCGTCATTGCTCTCTTCTCCTTTGAC‑TTCCGGTCAGTAAGAGGCACTCTACATGTGTTTAGCGTATAGGGGGcc > 2:381748/1‑251 (MQ=255) gtttgttggcctccccccccGCCGTGGCATTTACTCGGATTCTTAGGCCGTTAAAACAGCACCCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCA‑GCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCt < 1:462563/235‑1 (MQ=255) cTTATGGGATTACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCA‑GCGCCGCCACTACGGCGGTGTTAAAGATGGAGGTCAGGGGGGCCATCATCTCCTTTTGCTGCTTCATTTCTCTCTTTTCCTT‑GACCTTTCGGTCAGTTAAAGGCACTTTACATGTGTTCCACATATAAGGGGCCTCg > 2:100885/1‑251 (MQ=255) ggggcacccccccccGCCGTTCCACTTACTCGGATTCGTAAGCCGGAAAAACAGCAACCCCCGTCTGGCCGGTTCGGATGTGACCCTCACAGAGGTCTTTTCCCGTTACCA‑GCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGG‑t < 1:381748/240‑1 (MQ=255) gggcccccccccccGCCGTTGCACTTACTCGGTTTCGTAAGCCGTAAAAACAGCCACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCA‑GCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGG‑tt < 2:34132/241‑1 (MQ=255) cTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCA‑GCGCCGCCACTACGGCGCTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGG‑TTGATGGTAAAATATCACTCGGGGAtttt > 2:262001/1‑251 (MQ=255) cGTAAGCAGTAAAAACAGCAACCTCCGTCTCGCCATTTCGGATGTGATCCTCACGGAGGTCTTTCCCGGTTCCCA‑CCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGG‑TTGATAGTAAAATATCACTCGGGGCTTTTCTCTATCt < 2:105912/251‑1 (MQ=255) gTAAGCCGTGAAAACAGCACCCTCCGTCTGGCCAGTTCGGATGTGACCCTCACAGAGGTCTTTTCTCGTTCCCA‑GCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGG‑TTGATAGTAAAATATCACTCGGGGCTTTTCTCTATCTg < 1:99659/251‑1 (MQ=255) gccatttgggaattaacccccacaGAAGTCTTTTCTCGCT‑CCACGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCAGTTCTCTCTTCTCCTT‑GTCCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGTG‑TTGATAGTAAAATATCACGCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGAT‑AGcc < 1:434992/231‑1 (MQ=255) aTGTGAACCTCACAGAGGTCTTTTCTCGTTACCA‑GCGCCGCCACTACGGCGGCGATAAAAAACACGCTCAGGGCGGCAAACATCGCCTTATGCTGCTTTATTGCTCTCTTCTCCTT‑GAACTTACGGGCCGTAAGAGGCACTCTACATGTGTTCCGCATATAGGGGGCCTAGGG‑TTGATGGTAAAATATCACTCGTGGCTTTTTTCTTTCTGCCGTTCAGCCAATTCCTGAGACAGAC‑AGCCTCAAGCAccc > 2:190323/1‑251 (MQ=255) ttcccA‑GCCCCGCCCCTGCGGCGGTAATACAGAGGCCGACCAGGGCGACAATCACCTCCTTATGCGGTTTCATGCCTCTCTTCTCCTT‑GCCTTTACGGTCGGTAAGAGGCACTCCAAATGGGTTCACAATTTAGG‑GGCCCCGGGGTTGATAAAAAAATATCCCTGGGGTTTTTTCTCAACCTCCCGTTCAGCTAATTCCGGAAACAGAT‑ACCCTCAAGCACCCGCCGCTATTAT‑ATCGCTCTCTTTAAcc < 1:23256/247‑1 (MQ=255) ttACCA‑GCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGAAAATCCTCGCCTTATGCAGCTTCATTGCTCTCTTCTCCTT‑GACCTTTCGGTCCGTAAGAGGCACTCTACATGTGTTCTGCATATAGGGGGCCTCGGG‑GTGATAGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGGGCCA‑ACTAGTCTCAAGCACCCCCCCCT‑TTATTATCCCTCTCTTTTAccc > 2:203224/1‑251 (MQ=255) cgccACTACGGCGGTGATACAGATGACGATCAGGGGGACAATCATCGCCTTTTGCTGCTTCCTTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGG‑TTGATAGTAAAATATAACTCGGGGCTTTTCTCTTTCTGCCGTTTAGCTAATGCCTGCGACAGAT‑AGCCTCAAGCACCCGCCGCTATTAT‑ATCCCTCTCTTTAACCCAttctttttt > 2:459319/1‑244 (MQ=255) cggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGG‑TTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGAC‑AGCCTCAAGCACCCGCCGCTATTAT‑ATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTa > 2:190485/1‑250 (MQ=255) ggTGATACAGAGAAGGATCGGGGCAAAAATCATCGCCTTTTGCTGCTTCATTGCTCTCTTCCCCTT‑GCCCTGAGGGTCAGAAAGGGCCCCTCTACATGTGTTCAGCATATGGGGGGCCGGGGG‑TTAATAGAAAAATATCACTCGGGTTTTTTCTTTCTCTGCCGTTCAGCAAAGGCCTGAGACAGAT‑ACCCTCAACCACCCGCCGCTATTAT‑ATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAAt < 1:459319/251‑1 (MQ=255) cctTCATGGCTCCTTTCTCCTG‑GACCTATCGGTCAGTAAGAGGCCCTCTACATGTGTTCAGCATATGGGGGGTCTCGGG‑TTGATATTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGAT‑AGCCTAAAGCATCCGCCGCTATTAT‑ATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAtt < 2:236544/250‑1 (MQ=255) | GCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCA‑GCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTT‑GACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGG‑TTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGAC‑AGCCTCAAGCACCCGCCGCTATTAT‑ATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATT > NC_000913/16720‑17147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |