Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	15,073	A→T	L302L (CTA→CTT)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	15,073	0	A	T	98.4%	184.8 / NA	62	L302L (CTA→CTT)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (29/32);  minor base G (0/1);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGT  >  NC_000913/14828‑15319                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        gACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCg                                                                                                                                                                                                                                                    <  2:259855/251‑1 (MQ=255)           aCCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTGAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGCTGGTCGCGTCAAACTGAAAGAGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCggg                                                                                                                                                                                                                                          >  1:84427/1‑250 (MQ=255)            ccGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGAGCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTGGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCATACCGGTAAGCTTTTCCGTATGCGCGGTa                                                                                                                                                                                                                                         <  2:273772/251‑1 (MQ=255)                    gTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTc                                                                                                                                                                                                                                 >  1:22421/1‑251 (MQ=255)                          cGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGCGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCCAACTGAAAGTGCCTGGCGAAACCCCGACCGGTAAGCTTTTCCGTATGCGGGGGAAAGGCGTCAAGTCt                                                                                                                                                                                                                           >  2:436699/1‑251 (MQ=255)                          cGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGGAAAGGCGTCAAGTcg                                                                                                                                                                                                                           >  1:179613/1‑250 (MQ=255)                            ggCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGt                                                                                                                                                                                                                         >  1:338520/1‑251 (MQ=255)                                aaGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAAACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCcgc                                                                                                                                                                                                                     >  1:312258/1‑251 (MQ=255)                                 aGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCcgcg                                                                                                                                                                                                                    <  1:160821/251‑1 (MQ=255)                                 aGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCcgcg                                                                                                                                                                                                                    >  1:226452/1‑251 (MQ=255)                                   gTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGt                                                                                                                                                                                                                  <  1:389108/251‑1 (MQ=255)                                   gTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACAGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGAACGGTAAGCTTTTCCGTATTAGAGGTAAAGGCGTCAAGTCTGTCCGCGGt                                                                                                                                                                                                                  >  1:423225/1‑251 (MQ=255)                                     gAAGCGGGCGGGCATGGCGCACCGGCAGGCGATCTGGACGTTCAGGTTCAGGTTAAACAG‑CACCCGATGTTCGAGCGTGAAGGCAACAACCTCTACTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCGTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTGCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTgg                                                                                                                                                                                                                <  2:433006/251‑1 (MQ=255)                                               aGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGGTCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGGAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAgggg                                                                                                                                                                                                      >  1:338085/1‑250 (MQ=255)                                                    ggCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAttt                                                                                                                                                                                                 <  1:161976/251‑1 (MQ=255)                                                      cgcACCCGGAGGCGGTCTGTTCGTTCAAGTTTAGGTTAAACAGGCACC‑GGTTTTCGAACGTGAAGGCAACCACCTCTAATGCCAAGTTCCGATCCACTTTGGTATGGCGGCGGTGGGTTGCGAAAACGGAGTTCCGGACCTTGATGGTCGCGTCAAACTGAAAATGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGc                                                                                                                                                                                               <  1:104682/251‑1 (MQ=255)                                                        cACCGGCATGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCtg                                                                                                                                                                                             <  1:480803/251‑1 (MQ=255)                                                         cccGGCAGGCGATCTGTACGTTCAGGTCCAGGTTAAACAG‑CACCCGATTTTCGAGCGGGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCGGACCGGTAAGCTTTGCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCtgt                                                                                                                                                                                            <  1:467426/250‑1 (MQ=255)                                                            ggCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGcc                                                                                                                                                                                         >  1:422052/1‑251 (MQ=255)                                                                    aTCTGTACGTTCAGGTTAAGGTTAAACAG‑CACCCGATTTTCGAGCGTGACGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAATACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCCGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGCCGCGTTgtc                                                                                                                                                                                 >  1:430518/1‑251 (MQ=255)                                                                               caggttcaggttAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGACCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGCTGGTCGCGTCAAACTGAAAGTGCCTTGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGAGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGGTGTCATCGAAACAcc                                                                                                                                                                      >  2:363802/1‑251 (MQ=255)                                                                               caggtacaggttAAACAG‑CACCCTATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTTCGCTGTGGCGCACAGGGTGATTTGCTGTGCCTCGTTGTCGTCGAAacac                                                                                                                                                                       <  2:481109/250‑1 (MQ=255)                                                                                aggttcaggttAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGGAAGCTTTTCAGTATGCGCGGTAAAGGCGTCAAGACTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGCCGCGTTGTCGTCGaaaacccg                                                                                                                                                                     >  1:199661/1‑246 (MQ=255)                                                                                           aaaCAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGaa                                                                                                                                                          <  1:482783/251‑1 (MQ=255)                                                                                                  cACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGagaa                                                                                                                                                    <  1:60076/251‑4 (MQ=255)                                                                                                  cACCCGAGTTTCGAGCGTGAAGGCAACAACCTCTAATGCGAAGTCCCGATCAACTGCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGagaa                                                                                                                                                    <  2:193724/251‑4 (MQ=255)                                                                                                   aCCCAATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGagaag                                                                                                                                                   <  1:333074/251‑5 (MQ=255)                                                                                                                    gAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCCCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAAGCGTCAAGTCTGTCCGCGGGGGCGCACAGGGCGATTTGCTGTGCCGCGTTGTCGTCGAAACCCCGGTCGGCCTGTACGCGAAGCAGAAACAGCcgctgc                                                                                                                                   >  1:188790/1‑250 (MQ=255)                                                                                                                      aGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgcaa                                                                                                                                 >  1:435859/1‑250 (MQ=255)                                                                                                                         caacaaCCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAGGCAGAAACAGCTgctgcaagagc                                                                                                                             >  1:331800/1‑251 (MQ=255)                                                                                                                            caacCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgcaagagctgc                                                                                                                          >  1:128810/1‑251 (MQ=255)                                                                                                                                 tctATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGTGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCCCAGGGGGATTTGCTGTGCCGCGTTGTCGTCGCAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgcaagagctgcaaga                                                                                                                      >  2:504419/3‑250 (MQ=255)                                                                                                                                              cccGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGc                                                                                                        <  1:393782/250‑1 (MQ=255)                                                                                                                                                             tATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGCCGCGTTGTCGTCGGAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCCACCggtgggcac                                                                                         >  1:106283/1‑243 (MQ=255)                                                                                                                                                             tATGGCGGCGCTGGGGGGCGCAATCGAAGTACCCACCCTTGTTAGGCGCGTCCCACTGTAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGAGCGGTAAAGGCGGCAAGGCTGTCCGCGGGGGCGCACAGGGGGGTTTGCAGTTCCGCGGTGTCGGCGACACCCCGGGAGGCCTGAACGAGGAGCAGAAACAACTGCTGCAAGAACTGCAAGAAAGATTCGAGTGCCCCACCCggggacacc                                                                                         >  2:263975/1‑244 (MQ=255)                                                                                                                                                                ggcggcGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTTAAAGGGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCacaaca                                                                                     <  1:138933/251‑1 (MQ=255)                                                                                                                                                                  cggcgCGGGGTGGCGAAATCGAAGTACCGACCCGGGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGc                                                                                   <  2:284245/251‑1 (MQ=255)                                                                                                                                                                    gcgctggtgggggcattCGAAGTACCGACCCTTGATGGTCGCTTCCATCTGAAAGTTCCGGGCGAACCCCATACCGGTAATCTTTTCCGGATGCGCGGTAAAGGCGTCAAGTGTGTCCGCGGTGGCGCACAGTGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCATAAACATCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGTCCCAACCGGTTAGCACCACAGccc                                                                                 <  2:262905/235‑1 (MQ=255)                                                                                                                                                                    gcgcTGGGTGGCGAACTCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGccc                                                                                 <  1:261103/251‑1 (MQ=255)                                                                                                                                                                          ggTGGCGAATTCGAAGTACCGACCCTTCATGGTCGCGTCAAACCGAAAGGGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTTGCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTc                                                                           <  2:344743/251‑1 (MQ=255)                                                                                                                                                                           gTGGCGAAAGCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCa                                                                          <  2:422052/251‑1 (MQ=255)                                                                                                                                                                               cGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAGGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAaga                                                                      <  1:38990/251‑1 (MQ=255)                                                                                                                                                                                      gAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGcttctt                                                               >  2:124476/1‑251 (MQ=255)                                                                                                                                                                                         gTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGa                                                            <  2:188178/251‑1 (MQ=255)                                                                                                                                                                                            ccGACCCTTGAGGTTCGCGTCAAACTGAAAGTGCCTGGAGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGGAAATGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGGAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATgg                                                         <  2:476917/251‑1 (MQ=255)                                                                                                                                                                                               gcccTTGATGGCCGCCGAAAACGGAAATTGCTTGGAGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGgtgt                                                      <  2:137942/250‑1 (MQ=255)                                                                                                                                                                                               aCCCTTGGTGGTCGCGTCAAACTGACAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAGACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGgtgg                                                      >  2:247032/1‑250 (MQ=255)                                                                                                                                                                                               aCCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGgtgg                                                      >  2:31475/1‑250 (MQ=255)                                                                                                                                                                                                cccgtGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACACAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGtgtg                                                     <  1:401246/247‑1 (MQ=255)                                                                                                                                                                                                cccTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGtgtg                                                     <  2:331800/251‑1 (MQ=255)                                                                                                                                                                                                    tGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGGCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTgaa                                                   <  1:265518/249‑1 (MQ=255)                                                                                                                                                                                                    cgATGGACGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCTCAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTgaag                                                  <  1:24310/249‑1 (MQ=255)                                                                                                                                                                                                      aTGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAACGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTgaaga                                                 >  2:72484/1‑249 (MQ=255)                                                                                                                                                                                                       tGGTCGCGTCAAACTGAAGGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGt                                              >  1:123011/1‑251 (MQ=255)                                                                                                                                                                                                        ggTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTTTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGGTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGt                                              <  1:59544/250‑1 (MQ=255)                                                                                                                                                                                                                    cTGAAAGTGCCTGGAGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCAAACCGGTGAGCACAACAGACAGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCt                                 >  2:466806/1‑251 (MQ=255)                                                                                                                                                                                                                                  cGAAACCCAGACCGGTAAGCTTTTCCGTATGAGCGGTAAAGGCGTCAAGTCTGGCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTTATGGTGTGAAGAAGTTTTTAGACGACCTGACCCGCTAACCTc                   <  2:84457/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaccaatgccGGTAAGCTTTGCAGTATGCGCGGTCAAGGCGTCAAGGCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGGGCCGCGTGGTCGGCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGATGCAAGAAAGCGTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGGTTTCTGACGACCTGACCCGTTAACCTccc                 <  2:72753/242‑1 (MQ=255)                                                                                                                                                                                                                                     aaCCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTcccc                >  1:48852/1‑251 (MQ=255)                                                                                                                                                                                                                                       cccAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCa               >  1:403648/1‑250 (MQ=255)                                                                                                                                                                                                                                           gACCGGTAAGCTGTGCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCTCGCGCTCAAAGAGCTTCTTTGATGGTTTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGc          <  1:297420/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAACCTGCCCgg  >  2:265387/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        GACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCG‑GTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGT  >  NC_000913/14828‑15319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑