Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,956	G→C	L263L (CTG→CTC)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,956	0	G	C	100.0%	231.8 / NA	72	L263L (CTG→CTC)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (37/35);  total (37/35)

GACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGC  >  NC_000913/14710‑15200                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      gACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTAtt                                                                                                                                                                                                                                                   >  1:273772/1‑251 (MQ=255) gACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCAGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTAtt                                                                                                                                                                                                                                                   <  2:140232/251‑1 (MQ=255)  aCCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCACAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACCACCTCTATTg                                                                                                                                                                                                                                                  >  2:60076/1‑251 (MQ=255)   cctttCCACACTTTCATGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGGGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGc                                                                                                                                                                                                                                                 <  2:447582/247‑1 (MQ=255)    cTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACACATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGGGGACACTGGAGACCGCATCCGTCTTGCGGGCGACGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCg                                                                                                                                                                                                                                                >  1:84457/1‑251 (MQ=255)         cacaCTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTc                                                                                                                                                                                                                                           >  1:31765/1‑251 (MQ=255)            ccTGTCAGGGCCTCGGAACGCTGATCAGAGATCCGTGCAACAAATGTCATGATCATGGTCTGGTTGAGCGCAGCAAAACGCTGGCCGATAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTGTTGCGGGCGAAGTTGAAGCGGGCGAGCAGGGCGGACCGGTAGGCGATCTGGACGTCCAGGTTCATGTTAAACAG‑CATCTGATTTTCGTGCTTGAAGGCAACAACCTCTATTGCGAATTCCCg                                                                                                                                                                                                                                        <  2:114824/250‑1 (MQ=255)            acTGTCAGGTCCGCGTGACGCTGATCAAAGATCCGTGCAACAAATTTCATGTTCATGGTCGTGTGGAGCGCAGCAAAACGCTGTCCGTTAAAAGCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCATGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAATGCGATAACCTCTATTGCGTAGTCCCg                                                                                                                                                                                                                                        <  2:461679/251‑1 (MQ=255)               gTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAAGCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGAt                                                                                                                                                                                                                                      <  2:402433/250‑1 (MQ=255)                 cacggtcgcgTTACGCTTATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTTTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTTCGTTCAGGTTCATGTTAAACCG‑CACCCGATTTTCTAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCaa                                                                                                                                                                                                                                   <  1:38378/245‑1 (MQ=255)                 cAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCa                                                                                                                                                                                                                                    <  1:350193/250‑1 (MQ=255)                 cAGGGACGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATAGTCGTGTTGAGCGCAGCAAAACGATGTCAGTTAAAATCCCGGCAAGGGTGGACACTGGAGAACGCATCAGTATTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGAACCGGCAGGCGCTCTGTAAGTTCCGGTTCAGGTTAAACAG‑CACCCGATTTTAGAGCGTGAAGGCAACAACCTCAATTGCGAAGTccacatca                                                                                                                                                                                                                                    >  1:504265/1‑244 (MQ=255)                   gggttgcgGTTCGCTGATCAAAGATCCGTGCAAGAAATGTCATGGTCATGGTCGTGTTGATCGCAGCATAACGCTGTCCGTTAAATTCCCGGTAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGTGCGAAGTTGAAGCGTGCGAGCATGGCGCACCGGCTGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACATCCTCTATTGCGAAGTCCCTTTCAACt                                                                                                                                                                                                                                 <  2:416066/246‑1 (MQ=255)                   gggCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACt                                                                                                                                                                                                                                 >  1:151697/1‑251 (MQ=255)                        gcgGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCt                                                                                                                                                                                                                            <  1:497889/251‑1 (MQ=255)                           gTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGCTTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATg                                                                                                                                                                                                                         >  1:440014/1‑251 (MQ=255)                           gTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGGCGTGTTGAGCGCAGCAAAACGCTCTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGTGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTAt                                                                                                                                                                                                                          <  1:323994/250‑1 (MQ=255)                              cGCTGCTCAAAGATCCGTGCAAAAAATATCATGGTCATGGTCGTGTGGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGTGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTTCGTTCATGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTTTgg‑cg                                                                                                                                                                                                                     <  2:340176/251‑1 (MQ=255)                                   aTCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCt                                                                                                                                                                                                                >  1:88269/1‑251 (MQ=255)                                     cAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTg                                                                                                                                                                                                               <  1:139643/250‑1 (MQ=255)                                      aaaGATCCGTGCAACACATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGACGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGGGCGTGAAGGCACCAACCTCTATAGCGAAGTCCCGATCAACTTCGCTATGA‑CGGCGCTggg                                                                                                                                                                                                             >  1:408586/1‑251 (MQ=255)                                       aaGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CCCCCGATTTTCGAGCGTGAAGGCAACAACCTCTTTTGCGAAGTCCCGATCACCTTCGCTATGG‑CGGCGCTgggg                                                                                                                                                                                                            >  2:235756/1‑250 (MQ=255)                                        aGATCCGGGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAATCGCTGTCCGTTAAAATCCCGGCAGGGGGGGACACTGGAGACCGCATCCGTCTTGCGGTCGAAGGGGAAGCGGGCGAGCATGGCGCACCGGCGGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGAGTTTCGAGCGTGAGGGCAACAACCTCTATTGCGCAGTCCCGATCAACTTTGCTATGG‑CGGGGCTggggg                                                                                                                                                                                                           >  2:122538/1‑249 (MQ=255)                                            ccGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAACACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGAGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGGTCAGGTTAAAGAG‑CACCCGATTTTAGAGCGTGAAGGCAACAACCTCTATTGAGAAGTCCCGGTCAACTTCGCTATGG‑CGGCGCCGGGGGGCgc                                                                                                                                                                                                       >  1:226350/1‑250 (MQ=255)                                             cGTGCAACAAATGTCATGGTCAGGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGaa                                                                                                                                                                                                      <  2:151697/251‑1 (MQ=255)                                                   acaaatgtccgtgtcagtgtcGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGaa                                                                                                                                                                                                <  2:330622/233‑1 (MQ=255)                                                    caaATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAg                                                                                                                                                                                               <  2:119606/251‑1 (MQ=255)                                                           catggtcatggtCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGGGAAATCgcaataacgac                                                                                                                                                                                        >  2:160821/1‑241 (MQ=255)                                                           catggtcatggtCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGa                                                                                                                                                                                         <  2:363770/250‑1 (MQ=255)                                                              ggtcatggtcGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGAccc                                                                                                                                                                                      <  1:254403/250‑1 (MQ=255)                                                                  aagggtgtgtgtggcgcAGCAAAACGTTGTCCGTTAAAATCCCGGCAGGGGGGGACACTGGAGACCGCATCCGGCTGGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGGACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGAt                                                                                                                                                                                 <  2:252898/238‑1 (MQ=255)                                                                    ggtcGTGTTGAGCGCAGCAAACCGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGAGAAGCGGGCGAGCATAGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTGCGCTATGG‑CGGCGCCGGGTGGCGAAAACGAAAACCCGACCCTTGTTgg                                                                                                                                                                               >  2:138933/1‑251 (MQ=255)                                                                       cGTGTGGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTcg                                                                                                                                                                            <  2:31765/251‑1 (MQ=255)                                                                         tgtTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGCGCGTGACGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGAGCTGGGTGGCGAAATCGAACGACCGACCCTTgtagggctgg                                                                                                                                                                          >  2:100641/1‑242 (MQ=255)                                                                                  cagcaAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTAAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCCAACTg                                                                                                                                                                  >  1:280715/1‑250 (MQ=255)                                                                                               tCCGTTAAAATCCCGACAGGGGTGGACACTGGCGACCGCATCCGTCTTGCGGGCGCAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGGACGTTCAGGTTCAGGTTAAACAG‑CAACCGCTTTTCGAGCGTGAAGGCAACAACATCTATTCCGCAGTCCCGATCAACTTCGCTATGGATGGC‑CTGGGTGGCGCCAACGCAGAACCGACCCCTTATGGTcgcagcccaccaacctggccttgtgc                                                                                                                                                    >  2:201439/1‑228 (MQ=255)                                                                                                          cccGGCAGGGGTGGACACTGGAGACCTCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGATCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCTAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAACTGCCTGCCTAAACCCAGACCg                                                                                                                                         <  2:226350/251‑1 (MQ=255)                                                                                                           ccGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCgg                                                                                                                                        >  1:81234/1‑251 (MQ=255)                                                                                                                aGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGc                                                                                                                                   >  1:188178/1‑251 (MQ=255)                                                                                                                       gACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCg                                                                                                                            <  2:259855/251‑1 (MQ=255)                                                                                                                                 aCCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTGAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGCTGGTCGCGTCAAACTGAAAGAGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCggg                                                                                                                  >  1:84427/1‑250 (MQ=255)                                                                                                                                  ccGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGAGCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTGGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCATACCGGTAAGCTTTTCCGTATGCGCGGTa                                                                                                                 <  2:273772/251‑1 (MQ=255)                                                                                                                                          gTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTc                                                                                                         >  1:22421/1‑251 (MQ=255)                                                                                                                                                cGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGCGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCCAACTGAAAGTGCCTGGCGAAACCCCGACCGGTAAGCTTTTCCGTATGCGGGGGAAAGGCGTCAAGTCt                                                                                                   >  2:436699/1‑251 (MQ=255)                                                                                                                                                cGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGGAAAGGCGTCAAGTcg                                                                                                   >  1:179613/1‑250 (MQ=255)                                                                                                                                                  ggCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGt                                                                                                 >  1:338520/1‑251 (MQ=255)                                                                                                                                                      aaGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAAACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCcgc                                                                                             >  1:312258/1‑251 (MQ=255)                                                                                                                                                       aGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCcgcg                                                                                            >  1:226452/1‑251 (MQ=255)                                                                                                                                                       aGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCcgcg                                                                                            <  1:160821/251‑1 (MQ=255)                                                                                                                                                         gTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGt                                                                                          <  1:389108/251‑1 (MQ=255)                                                                                                                                                         gTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACAGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGAACGGTAAGCTTTTCCGTATTAGAGGTAAAGGCGTCAAGTCTGTCCGCGGt                                                                                          >  1:423225/1‑251 (MQ=255)                                                                                                                                                           gAAGCGGGCGGGCATGGCGCACCGGCAGGCGATCTGGACGTTCAGGTTCAGGTTAAACAG‑CACCCGATGTTCGAGCGTGAAGGCAACAACCTCTACTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCGTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTGCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTgg                                                                                        <  2:433006/251‑1 (MQ=255)                                                                                                                                                                     aGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGGTCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGGAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAgggg                                                                              >  1:338085/1‑250 (MQ=255)                                                                                                                                                                          ggCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAttt                                                                         <  1:161976/251‑1 (MQ=255)                                                                                                                                                                            cgcACCCGGAGGCGGTCTGTTCGTTCAAGTTTAGGTTAAACAGGCACC‑GGTTTTCGAACGTGAAGGCAACCACCTCTAATGCCAAGTTCCGATCCACTTTGGTATGG‑CGGCGGTGGGTTGCGAAAACGGAGTTCCGGACCTTGATGGTCGCGTCAAACTGAAAATGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGc                                                                       <  1:104682/251‑1 (MQ=255)                                                                                                                                                                              cACCGGCATGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCtg                                                                     <  1:480803/251‑1 (MQ=255)                                                                                                                                                                               cccGGCAGGCGATCTGTACGTTCAGGTCCAGGTTAAACAG‑CACCCGATTTTCGAGCGGGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCGGACCGGTAAGCTTTGCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCtgt                                                                    <  1:467426/250‑1 (MQ=255)                                                                                                                                                                                  ggCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGcc                                                                 >  1:422052/1‑251 (MQ=255)                                                                                                                                                                                    cAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGCTTTTCGAGCGTGAAGGCACCAACCTCTCTTGCGAAGTCACGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGCAGt                                                                                                                                                                                              >  2:232930/1‑124 (MQ=255)                                                                                                                                                                                    cAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGt                                                                                                                                                                                              <  1:232930/124‑1 (MQ=255)                                                                                                                                                                                          aTCTGTACGTTCAGGTTAAGGTTAAACAG‑CACCCGATTTTCGAGCGTGACGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAATACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCCGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGCCGCGTTgtc                                                         >  1:430518/1‑251 (MQ=255)                                                                                                                                                                                                     caggttcaggttAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGACCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGCTGGTCGCGTCAAACTGAAAGTGCCTTGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGAGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGGTGTCATCGAAACAcc                                              >  2:363802/1‑251 (MQ=255)                                                                                                                                                                                                     caggtacaggttAAACAG‑CACCCTATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTTCGCTGTGGCGCACAGGGTGATTTGCTGTGCCTCGTTGTCGTCGAAacac                                               <  2:481109/250‑1 (MQ=255)                                                                                                                                                                                                      aggttcaggttAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGGAAGCTTTTCAGTATGCGCGGTAAAGGCGTCAAGACTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGCCGCGTTGTCGTCGaaaacccg                                             >  1:199661/1‑246 (MQ=255)                                                                                                                                                                                                                 aaaCAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGaa                                  <  1:482783/251‑1 (MQ=255)                                                                                                                                                                                                                        cACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGagaa                            <  1:60076/251‑4 (MQ=255)                                                                                                                                                                                                                        cACCCGAGTTTCGAGCGTGAAGGCAACAACCTCTAATGCGAAGTCCCGATCAACTGCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGagaa                            <  2:193724/251‑4 (MQ=255)                                                                                                                                                                                                                         aCCCAATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGagaag                           <  1:333074/251‑5 (MQ=255)                                                                                                                                                                                                                                          gAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCCCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAAGCGTCAAGTCTGTCCGCGGGGGCGCACAGGGCGATTTGCTGTGCCGCGTTGTCGTCGAAACCCCGGTCGGCCTGTACGCGAAGCAGAAACAGCcgctgc           >  1:188790/1‑250 (MQ=255)                                                                                                                                                                                                                                            aGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgcaa         >  1:435859/1‑250 (MQ=255)                                                                                                                                                                                                                                               caacaaCCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAGGCAGAAACAGCTgctgcaagagc     >  1:331800/1‑251 (MQ=255)                                                                                                                                                                                                                                                  caacCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgcaagagctgc  >  1:128810/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAG‑CACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGC  >  NC_000913/14710‑15200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑