Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,698	C→T	F177F (TTC→TTT)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,698	0	C	T	95.7%	200.2 / NA	69	F177F (TTC→TTT)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (33/33);  minor base G (1/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGT  >  NC_000913/14462‑14941                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    ggCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGAc                                                                                                                                                                                                                                       >  1:466678/1‑251 (MQ=255)             ggcggcGGACTTGGTCGTCAACGTGCTGCGCGCGGTGCTGAATTACTCTAGATCAGGGAGTGCACCCTCGAAGAAGCTGGACGTGGCGTGACCAAAGAGATCCGCATTCCGTCTCTGGAAGAGTGTTACGTTGGTCACGGTAGCGGTGCAAAACAAGGTACACAGCCGCAGACCTGTCCGCCCTGTCATGGTTCTGGTCAGGTGCAGATGCTTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTg                                                                                                                                                                                                                           <  2:302240/251‑1 (MQ=255)                      cGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAACCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCTGTGCAGCAGACCTGTCCACACTGTCAGGGCcg                                                                                                                                                                                                                  >  2:139643/1‑251 (MQ=255)                         ggTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCgg                                                                                                                                                                                                               >  1:426028/1‑251 (MQ=255)                             gtcAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACg                                                                                                                                                                                                           <  2:346059/251‑1 (MQ=255)                               gaaCGTGCGGCGCGCGGTGATGATTTACGCTATAACATGGAGCACACCCTCGACGCAGCTGCGCGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGATGAGTGTGACGTTTGCCACGGTAGCGGCGGAAAACCAGGCAAACAGCCGCAGACCTGTCCGAACTGACATGCGTCTGGTCAGGTGCAGGTGCGTCAGGGCTTCTTGGCCGTGCAGCGAACCTGTCCACACTATCAGGCCCGGGGTACg                                                                                                                                                                                                           >  2:24310/2‑249 (MQ=255)                                      cggcgCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCaa                                                                                                                                                                                                   >  1:365702/1‑250 (MQ=255)                                              ggTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTg                                                                                                                                                                                          >  1:284245/1‑251 (MQ=255)                                              ggTGCTGATTTACGCTAGAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGAGCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGGACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTg                                                                                                                                                                                          <  2:216385/251‑1 (MQ=255)                                                  cagattgacggtagaaCACGGAGCTCACCCCCGAAGAAGCTGTACGTGGCTGGACCAAAGAGAGCCGCATTCCGACTCTGGAAGGGCGGGACGTTTGCCACGGTTGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGCCCGGCCTGTCATGGTTCTGGTCAGGTGCAGATGCGGCAGGGATTCTTTGCCGTGCAGGAGACCTGGCCACACTGGCAGGGCCGCGGTACGCTGATCAAATATCCGTGcatc                                                                                                                                                                                      <  2:164501/237‑3 (MQ=255)                                                    gATTTACGCTATAACATGGAGCTCACTCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATGCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGcaacaa                                                                                                                                                                                    <  2:47375/251‑1 (MQ=255)                                                    gATTTACGCTATAACATGGAGCTCAACCTCGAAGAAGCTGTAAGTGGCGTGACCAAAGAGATCCGCCTTCCGACTCTGGAAGAGTGTGACGGTTGCCACGGTAGCGGTGCAAAACTAGGTACACAGCCGCAGGCCTGTCCGACCTGTCATGGGTCCGGTCATGTGCAGATGCGGCAGGGATTCTTGGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGTTACGCTGATCAAATATCCGTGcaacaa                                                                                                                                                                                    <  2:73375/251‑1 (MQ=255)                                                       ttACGCTATAACATGGAGCTCACACTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAAt                                                                                                                                                                                  <  2:352310/250‑1 (MQ=255)                                                         aCGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGATCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtc                                                                                                                                                                               >  1:440539/1‑251 (MQ=255)                                                          cgctagaacagtgAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGATATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCATATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCGGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtca                                                                                                                                                                              <  2:460792/239‑1 (MQ=255)                                                           gCTATAACAGGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGGGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtcat                                                                                                                                                                             <  2:365702/251‑1 (MQ=255)                                                                 aCATGGAGCCCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtcatggtcat                                                                                                                                                                       <  2:197633/251‑1 (MQ=255)                                                                    tGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTcatggtcatggt                                                                                                                                                                    >  1:476917/1‑251 (MQ=255)                                                                      gAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCAGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCTGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCg                                                                                                                                                                  <  1:128996/251‑1 (MQ=255)                                                                        gCTCACCCTCGAAGAAGCTGTACGTGGCGTGCCCAAAGCGATCCGCATTCCGACTCTGGCAGCGTGTGACGTTTGCCACGGTAGCGGTGCAACACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCCGCCCTGACCACACTGTCAGGGCCGCGGGCCGCTGATCACAGATCCGAGCAACCAATgccatggtcaaggcggtg                                                                                                                                                                >  2:261103/1‑243 (MQ=255)                                                                         cTCACCCTCGAAGAGGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGCCCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGGACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCgtgt                                                                                                                                                               >  2:393782/1‑251 (MQ=255)                                                                          gcACCCTCGAAGAAGCTGTACGTGGCGTGGCCAAGCAGATCGGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAACACCCGGTACACAGCCGCAGACCTGTCAGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTTCGATGATAACAGAGCCGTGCACCGAATgtcatgtgcatggcagtgtt                                                                                                                                                              >  2:41993/2‑237 (MQ=255)                                                                              ccTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAgc                                                                                                                                                          <  1:425599/251‑1 (MQ=255)                                                                                 cGAAGAAGCTGTACGGGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAgcg                                                                                                                                                         <  2:138341/249‑1 (MQ=255)                                                                                   aagaagCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCgcagc                                                                                                                                                     >  2:184060/1‑251 (MQ=255)                                                                                    agaagCGGTACGTGGCGTGACCAAAGAGATCCGCATGCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCgcagca                                                                                                                                                    <  2:466678/251‑1 (MQ=255)                                                                                      aagCTGTACGTGGCGTGCCCAAAGAGATCCGCATTCCGACTCTGGCAGAGTGTGACGCTTGCCACGGTAGCGGTGAAAAACCAGGTACACAGCAGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGAGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGCACGCTGATCAAAGATCCGCGCAACAATTGTCATGGGCATGGTCGTGTTGTGCGCAGCaac                                                                                                                                                  >  2:467426/1‑250 (MQ=255)                                                                                      aagCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCaaa                                                                                                                                                  <  1:498596/251‑1 (MQ=255)                                                                                        gCTGTACGTGGCGTGACCAAAGAGATCCGCTTTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTGCACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATTGTCATGGTCGTGTTGAGCGCAGCAAAAc                                                                                                                                                <  1:97247/251‑1 (MQ=255)                                                                                        gCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAAc                                                                                                                                                <  2:237346/251‑1 (MQ=255)                                                                                           gTACGGGGCGTTACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCt                                                                                                                                             <  2:354522/251‑1 (MQ=255)                                                                                                tggggcgACCAAAGAGAGCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAACCCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTGCGCTGATCAAAGATCCGTGCAACAAATGTCATGGACATGGTCGTGTTGAGCGCAGCAAGAAGCTGTCCg                                                                                                                                        >  2:333074/7‑251 (MQ=255)                                                                                                   cGTGACCAAAGAGATCCGCATTCCGACTCTGGCAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAACCGCTGCCCGtt                                                                                                                                      >  2:482783/1‑250 (MQ=255)                                                                                                      gACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCGCGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTaaa                                                                                                                                   <  1:460188/250‑1 (MQ=255)                                                                                                               gaTCCGCATTCCGACTCTGGAAGAGTGTGACGGTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAg                                                                                                                         <  1:450241/251‑1 (MQ=255)                                                                                                                          cGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACt                                                                                                              <  1:184060/251‑1 (MQ=255)                                                                                                                          cGACTCTGGAAGAGTGTGACGTTTACCACGGTAGCGTTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCCTGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGAGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCTTGTTGGGCGCAGCAAAACGCTGTCCGTAAAAATACCGGCGGGGGTGGacacg                                                                                                              >  2:401246/1‑250 (MQ=255)                                                                                                                           gACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTCCGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGGCACTg                                                                                                             >  2:15134/1‑251 (MQ=255)                                                                                                                              tctGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtcatggtcatg                                                                                                                                                                      >  1:105879/1‑191 (MQ=255)                                                                                                                              tctGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtcatggtcatg                                                                                                                                                                      <  2:105879/191‑1 (MQ=255)                                                                                                                                    agagTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGc                                                                                                     >  1:363770/1‑250 (MQ=255)                                                                                                                                          tgACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCGGCAAAACGCTGTCCGTTAAAATCCCGGCCGGGGGGGACACTGGAGACCGCaccagtc                                                                                              >  2:104682/1‑245 (MQ=255)                                                                                                                                                 tGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCggg                                                                                       >  1:481109/1‑251 (MQ=255)                                                                                                                                                 tGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCgg                                                                                        <  1:502216/250‑1 (MQ=255)                                                                                                                                                      cGGTAGCGGTGCAAAAACAGGTACACAGCAGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTAATGGTCGTGTTGAGCGCGACAACACGCTGTCCGTTACAATCCCGGCATGGGTGGGCACTGGGGGCCGCATCCGTCTTGCGGGCGagg                                                                                  >  2:48435/1‑249 (MQ=255)                                                                                                                                                         tAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTg                                                                               >  1:330622/1‑251 (MQ=255)                                                                                                                                                          aGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATccc                                                                                                                              >  1:197913/1‑203 (MQ=255)                                                                                                                                                          aGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGGCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATccc                                                                                                                              <  2:197913/203‑1 (MQ=255)                                                                                                                                                               tGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCgg                                                                         >  1:465633/1‑251 (MQ=255)                                                                                                                                                                    aaCCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAg                                                                    <  2:426028/251‑1 (MQ=255)                                                                                                                                                                        aGGTACACAGCCGCATACCTGTCCGCCCTGTCATGGTTCTGTTGAGGTGCAGATGTGTCAGGGATTCTTTGCCGTGTAGCTGACCGGTCCACACTGTCAGGGCCGCGGTACGCTGATCAACGATCCGTGCAACAAATGTCATGGTCATGGGTGTTGTGAGTGCAGCAAAACGCTGTCCGGTAAAATCCTGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCgcgaatg                                                                <  2:416153/251‑7 (MQ=255)                                                                                                                                                                                aGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGGGGACACTGGAGACCGAATCCGTCTTGCGGGCGGAGGGGAAGCGGGCGGGCATGGCGCCCCg                                                        >  2:160874/1‑251 (MQ=255)                                                                                                                                                                                          ctGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGACGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATc                                              >  1:433006/2‑251 (MQ=255)                                                                                                                                                                                              ccGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTa                                          <  1:458677/251‑1 (MQ=255)                                                                                                                                                                                                gACCTGTCATGGTTCTGGTCAGGTGCATATGCGTCAGGGATTCATGGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCTGTACGCGGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCTCACCGGCAGGCGATCTGTACg                                        <  2:464669/251‑1 (MQ=255)                                                                                                                                                                                                gACCTGTCATGGTTCTGGACAGATGAAGATGCGTCAGGGCTTCTTTGCCGAGCAGCAGACCTGTCCAAACTGTCAGGGCCGCGGTACGCTGATCACAGATCCGTGCAACCACTGTCATGGTCATGGTCGTGTTTAGCGCAGCACAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGCGACCGCATCCGTCTTGCGGGCGACGGCGCCGCGGGCGGGCATGGCGACCCGGCATGCgctccgtccg                                        >  2:59544/1‑242 (MQ=255)                                                                                                                                                                                                 aCCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACgt                                       >  1:193724/1‑251 (MQ=255)                                                                                                                                                                                                   cTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACgttc                                     >  1:119606/1‑251 (MQ=255)                                                                                                                                                                                                     gTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACgttc                                     >  1:259855/1‑249 (MQ=255)                                                                                                                                                                                                       cATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACgttcaggt                                 >  1:159008/1‑251 (MQ=255)                                                                                                                                                                                                          ggTTCTGGTCAGGTGCAGATGGGTCAGGGATTCTTTGCCGTGCAGCAGCCCTGTCCACTCTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGGGCAACAAAGGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACgttcaggttca                              <  2:300110/251‑1 (MQ=255)                                                                                                                                                                                                                   cAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAAc                      <  1:15134/250‑1 (MQ=255)                                                                                                                                                                                                                    aGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAg                    <  1:478831/251‑1 (MQ=255)                                                                                                                                                                                                                    aGGTGCAGAGGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACTCTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAg                    <  2:396825/251‑1 (MQ=255)                                                                                                                                                                                                                            atgcttcAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGTTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAAGCCCGGCAGGGGTGGACACTGGAGACCGCAGCCGTCTTGCGGGCGAAGGTGAAGCGGTCGATCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGAt            <  2:430774/245‑1 (MQ=255)                                                                                                                                                                                                                            aTGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGAt            <  1:441951/251‑1 (MQ=255)                                                                                                                                                                                                                                  cAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCgg      >  1:344743/1‑250 (MQ=255)                                                                                                                                                                                                                                    gggATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTAAGTTCAGGTTCAGGTAAAACAGCACCCGATTTTCGAGc    >  1:363208/1‑251 (MQ=255)                                                                                                                                                                                                                                      gATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGAGCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGt  <  1:160874/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    GGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGT  >  NC_000913/14462‑14941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑