Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,439	C→G	S116S (TCG→TCC)	yaaI ←	DUF2541 domain‑containing protein YaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,439	0	C	G	90.5%	120.8 / 6.4	42	S116S (TCG→TCC)	yaaI	DUF2541 domain‑containing protein YaaI
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base G (17/21);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-01

CCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCG  >  NC_000913/11196‑11683                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ccTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAgg                                                                                                                                                                                                                                               <  1:388152/251‑1 (MQ=255)         cGATGTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGTGAAAGTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGAttttt                                                                                                                                                                                                                                       <  1:366329/251‑1 (MQ=255)          gATTTAAACAGGTTGGTGCGTATCAGTACGCGGGAGAAGATAGTTTTGACTTTTTCATTATGGGTGAGCAATCGGGCGAAAGTTGCAAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATATGGATTATTTAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCGGGCGTTAGTCGTCGCCGATAATGTTCAGCGTGGCTATATCCGATGAGTTCAACGTATGACCGGAAAAGGTGATTTTTg                                                                                                                                                                                                                                      <  2:351129/251‑1 (MQ=255)                 aCAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGTAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTAAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGAGTTAGTCGTCGCCGATAATTTTCAGCGAGGCCATATCCGGTGAGTTCACCGTATGACCGGAAAAGGGGGATTTTGAGACGcc                                                                                                                                                                                                                               >  1:93889/1‑250 (MQ=255)                                        cTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCCACTGTTCCTCACTACCATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATTATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAACGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATcacttt                                                                                                                                                                                                        >  1:141778/1‑246 (MQ=255)                                        cTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGt                                                                                                                                                                                                        >  1:305758/1‑251 (MQ=255)                                                   aGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATc                                                                                                                                                                                             <  1:302540/251‑1 (MQ=255)                                                   aGTTTTGCCTTTTTCATTATGGGGGAGCAATCGGGCGAAATTTGCTAACTGTTCCTCACTACAATTCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATc                                                                                                                                                                                             <  2:406614/251‑1 (MQ=255)                                                      tttGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGGGGTTTTTGAGACGCAGCGTTTATTTTCGTTATCGCTGTTAATGTTTATcc                                                                                                                                                                                            >  2:235346/1‑249 (MQ=255)                                                        tGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCcagt                                                                                                                                                                                         <  1:465603/250‑1 (MQ=255)                                                         gCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCcagtc                                                                                                                                                                                        <  1:1825/250‑1 (MQ=255)                                                            tttttCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAACGGTGCTTTTTGAGACGCAGCGTTTCTTGTCGTTATCATTGTTATTGTTGTTCCGGTCAGTg                                                                                                                                                                                    >  2:118942/1‑251 (MQ=255)                                                             ttttCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCcagtcagt                                                                                                                                                                                     <  1:72271/249‑1 (MQ=255)                                                             ttttCATTATGGGTGAGAAATTGGGCTAAATTGTCCAACTTTGCCACACTACAAGGCTTAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGGTTCTATATTCGGGCGTTATTCGTCGCTGATAATTTTCAGCGTGGTCATATCCGATTAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGGTTATGGGCGTTATCACTGTTAATGTTGACCCAGTCAGTg                                                                                                                                                                                    <  2:311120/250‑1 (MQ=255)                                                                tCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCTCTGTTAATGTTGATCCAGTCAGTGGtt                                                                                                                                                                                 <  1:200077/250‑1 (MQ=255)                                                                       tggggAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAATAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCctt                                                                                                                                                                          <  2:220465/246‑1 (MQ=255)                                                                                aTCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTc                                                                                                                                                                >  1:231937/1‑251 (MQ=255)                                                                                  cGGGCGAAATTTGCCAACTGTTCCTCACTACAATGTGGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAAGTTTCAGCTTTGCCATTTCCGATGAGTTCACCGTATTGCCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATTACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCt                                                                                                                                                               <  1:207401/250‑1 (MQ=255)                                                                                                    tGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTg                                                                                                                                                                                                         >  1:364850/1‑190 (MQ=255)                                                                                                    tGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTg                                                                                                                                                                                                         <  2:364850/190‑1 (MQ=255)                                                                                                                     cTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGggcg                                                                                                                           >  1:238904/1‑251 (MQ=255)                                                                                                                           aaaaTCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTg                                                                                                                     >  1:301696/1‑251 (MQ=255)                                                                                                                            aaaTCCAGATCTGAATCATTCAGGTAAATAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGa                                                                                                                    <  2:338026/251‑1 (MQ=255)                                                                                                                                 cAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGCTAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTTTCGCTGTTAATGTTTATCCAGTCAGGGGTTTGCCCTTTTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATa                                                                                                                >  2:190788/1‑250 (MQ=255)                                                                                                                                            cATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGTAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCAcc                                                                                                    <  1:62587/251‑1 (MQ=255)                                                                                                                                               tCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGCAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGACGGCATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCt                                                                                                 >  1:464684/1‑251 (MQ=255)                                                                                                                                                     aaTTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGACGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTg                                                                                           >  2:198659/1‑251 (MQ=255)                                                                                                                                                      aTTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTg                                                                                                                                                                                    <  2:217313/161‑1 (MQ=255)                                                                                                                                                      aTTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTg                                                                                                                                                                                    >  1:217313/1‑161 (MQ=255)                                                                                                                                                           cattcattTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAAGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATc                                                                                     <  1:235346/251‑1 (MQ=255)                                                                                                                                                            attcattTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGCTGCACCGCTTAACTGTAAATCg                                                                                    >  1:434008/1‑251 (MQ=255)                                                                                                                                                                  tttttGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATg                                                                               <  1:108081/250‑1 (MQ=255)                                                                                                                                                                             tCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCagaga                                                                    <  1:156886/250‑1 (MQ=255)                                                                                                                                                                                             aGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCTATGAGTTCACCGTATGACCGGAAAAGGTGATTGTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTcac                                                    <  2:227001/250‑1 (MQ=255)                                                                                                                                                                                                cgtcgCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATg                                                <  1:106425/251‑1 (MQ=255)                                                                                                                                                                                                 gtcgCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGc                                               >  1:375659/1‑251 (MQ=255)                                                                                                                                                                                                       gATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGATGGAATATTAAGACTCTGACTGGCGCAACGGGCGGCTTTGAAATAAACAGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACaa                                          >  2:405988/1‑250 (MQ=255)                                                                                                                                                                                                       gATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACaaa                                         <  2:305758/251‑1 (MQ=255)                                                                                                                                                                                                        aTAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGGGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAAc                                        >  2:221978/1‑251 (MQ=255)                                                                                                                                                                                                                                   gAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGt             >  1:30140/1‑251 (MQ=255)                                                                                                                                                                                                                                           cGTATGACCGGACAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTAc     >  2:106119/1‑251 (MQ=255)                                                                                                                                                                                                                                              aTGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTAcgcg  <  1:190788/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCG  >  NC_000913/11196‑11683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑