Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,366	C→T	intergenic (‑10/+16)	yaaW ← / ← yaaI	putative enzyme‑specific chaperone YaaW/DUF2541 domain‑containing protein YaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,366	0	C	T	93.6%	126.5 / 0.6	48	intergenic (‑10/+16)	yaaW/yaaI	putative enzyme‑specific chaperone YaaW/DUF2541 domain‑containing protein YaaI
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (17/27);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.21e-01

CCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATG  >  NC_000913/11133‑11606                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  ccaccaATATGCTGTAATTCGCCAGCAATCAGCTGCCAGTTGCTGCGATGTTGCTCGGGATGCCCTTCCATCTATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGTCGAGTTGTGCCTTTGTCATTATGGGTGAGCAATCGTGCTAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTTTGGTTTCTATATTCTGGCGtt                                                                                                                                                                                                                                  <  2:29291/251‑1 (MQ=255)     caAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAgtc                                                                                                                                                                                                                              <  1:48047/251‑1 (MQ=255)     caAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAgtc                                                                                                                                                                                                                              <  1:401600/251‑1 (MQ=255)         aTGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGtcgtcg                                                                                                                                                                                                                          <  2:79666/251‑1 (MQ=255)         aTGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGtcgtcg                                                                                                                                                                                                                          >  1:79666/1‑251 (MQ=255)                 aTTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAAt                                                                                                                                                                                                                  >  1:80548/1‑251 (MQ=255)                              cAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGACCTTCCATCGATTTACCCAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGcc                                                                                                                                                                                                    >  2:62587/1‑251 (MQ=255)                                gCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCat                                                                                                                                                                                                  <  1:416554/251‑1 (MQ=255)                                gCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCat                                                                                                                                                                                                  <  1:360396/251‑1 (MQ=255)                                       gTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGAt                                                                                                                                                                                           <  1:337739/251‑1 (MQ=255)                                         ggcggcgATGTTGCTCGGGATTCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTTAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCGGGCGTTAGTCGTCGCCTATAATTGT‑CAGCGTGGCCATATCCGATg                                                                                                                                                                                          <  2:94512/249‑1 (MQ=255)                                            ggcgATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTTTCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTAATTATGGGTGAGCAATCGGGCGACATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGCGGTTTCTATATTCTGGAGTTATTCGTCTCCG‑TAATTTTTCTGCTTGGCTATATCCGTTGAGtt                                                                                                                                                                                      >  2:239905/1‑251 (MQ=255)                                               gATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCAc                                                                                                                                                                                   <  1:443387/251‑1 (MQ=255)                                                          ggATGCCCTTCCAACGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGAccgg                                                                                                                                                                        <  2:334339/251‑3 (MQ=255)                                                                ccTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAgg                                                                                                                                                                  <  1:388152/251‑1 (MQ=255)                                                                        cGATGTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGTGAAAGTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGAttttt                                                                                                                                                          <  1:366329/251‑1 (MQ=255)                                                                         gATTTAAACAGGTTGGTGCGTATCAGTACGCGGGAGAAGATAGTTTTGACTTTTTCATTATGGGTGAGCAATCGGGCGAAAGTTGCAAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATATGGATTATTTAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCGGGCGTTAGTCGTCGCCGATAATGTT‑CAGCGTGGCTATATCCGATGAGTTCAACGTATGACCGGAAAAGGTGATTTTTg                                                                                                                                                         <  2:351129/251‑1 (MQ=255)                                                                                aCAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGTAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTAAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGAGTTAGTCGTCGCCGATAATTTT‑CAGCGAGGCCATATCCGGTGAGTTCACCGTATGACCGGAAAAGGGGGATTTTGAGACGcc                                                                                                                                                  >  1:93889/1‑250 (MQ=255)                                                                                                       cTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCCACTGTTCCTCACTACCATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATTATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAACGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATcacttt                                                                                                                           >  1:141778/1‑246 (MQ=255)                                                                                                       cTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGt                                                                                                                           >  1:305758/1‑251 (MQ=255)                                                                                                                  aGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATc                                                                                                                <  1:302540/251‑1 (MQ=255)                                                                                                                  aGTTTTGCCTTTTTCATTATGGGGGAGCAATCGGGCGAAATTTGCTAACTGTTCCTCACTACAATTCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATc                                                                                                                <  2:406614/251‑1 (MQ=255)                                                                                                                     tttGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGGGGTTTTTGAGACGCAGCGTTTATTTTCGTTATCGCTGTTAATGTTTATcc                                                                                                               >  2:235346/1‑249 (MQ=255)                                                                                                                       tGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCcagt                                                                                                            <  1:465603/250‑1 (MQ=255)                                                                                                                        gCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCcagtc                                                                                                           <  1:1825/250‑1 (MQ=255)                                                                                                                           tttttCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAACGGTGCTTTTTGAGACGCAGCGTTTCTTGTCGTTATCATTGTTATTGTTGTTCCGGTCAGTg                                                                                                       >  2:118942/1‑251 (MQ=255)                                                                                                                            ttttCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCcagtcagt                                                                                                        <  1:72271/249‑1 (MQ=255)                                                                                                                            ttttCATTATGGGTGAGAAATTGGGCTAAATTGTCCAACTTTGCCACACTACAAGGCTTAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGGTTCTATATTCGGGCGTTATTCGTCGCTGATAATTTT‑CAGCGTGGTCATATCCGATTAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGGTTATGGGCGTTATCACTGTTAATGTTGACCCAGTCAGTg                                                                                                       <  2:311120/250‑1 (MQ=255)                                                                                                                               tCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCTCTGTTAATGTTGATCCAGTCAGTGGtt                                                                                                    <  1:200077/250‑1 (MQ=255)                                                                                                                                      tggggAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAATAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCctt                                                                                             <  2:220465/246‑1 (MQ=255)                                                                                                                                               aTCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTg                                                                                                                                                                                                                                       <  1:105918/104‑1 (MQ=255)                                                                                                                                               aTCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTg                                                                                                                                                                                                                                       >  2:105918/1‑104 (MQ=255)                                                                                                                                               aTCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTc                                                                                   >  1:231937/1‑251 (MQ=255)                                                                                                                                                 cGGGCGAAATTTGCCAACTGTTCCTCACTACAATGTGGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAAGTTT‑CAGCTTTGCCATTTCCGATGAGTTCACCGTATTGCCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATTACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCt                                                                                  <  1:207401/250‑1 (MQ=255)                                                                                                                                                                   tGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTg                                                                                                                            >  1:364850/1‑190 (MQ=255)                                                                                                                                                                   tGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTg                                                                                                                            <  2:364850/190‑1 (MQ=255)                                                                                                                                                                                    cTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGggcg                                              >  1:238904/1‑251 (MQ=255)                                                                                                                                                                                          aaaaTCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTg                                        >  1:301696/1‑251 (MQ=255)                                                                                                                                                                                           aaaTCCAGATCTGAATCATTCAGGTAAATAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGa                                       <  2:338026/251‑1 (MQ=255)                                                                                                                                                                                                cAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGCTAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTTTCGCTGTTAATGTTTATCCAGTCAGGGGTTTGCCCTTTTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATa                                   >  2:190788/1‑250 (MQ=255)                                                                                                                                                                                                           cATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGTAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCAcc                       <  1:62587/251‑1 (MQ=255)                                                                                                                                                                                                              tCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGCAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGACGGCATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCt                    >  1:464684/1‑251 (MQ=255)                                                                                                                                                                                                                    aaTTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGACGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTg              >  2:198659/1‑251 (MQ=255)                                                                                                                                                                                                                     aTTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTg                                                                                                       <  2:217313/161‑1 (MQ=255)                                                                                                                                                                                                                     aTTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTg                                                                                                       >  1:217313/1‑161 (MQ=255)                                                                                                                                                                                                                          cattcattTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAAGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATc        <  1:235346/251‑1 (MQ=255)                                                                                                                                                                                                                           attcattTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGCTGCACCGCTTAACTGTAAATCg       >  1:434008/1‑251 (MQ=255)                                                                                                                                                                                                                                 tttttGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATg  <  1:108081/250‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  CCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATG  >  NC_000913/11133‑11606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑