Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,225	C→T	M44I (ATG→ATA)  R132R (CGC→CGT)	yaaW ← mbiA →	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,225	0	C	T	98.2%	172.4 / NA	56	M44I (ATG→ATA)  R132R (CGC→CGT)	yaaW mbiA	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/33);  minor base A (1/0);  total (23/33)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCA  >  NC_000913/10980‑11462                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               catgtgttACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTGCAGCTTCAATCTTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCAGCGATTTAAACAGTTCGTTGCGTATCat                                                                                                                                                                                                                                           <  1:273054/245‑2 (MQ=255) cAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGGCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAg                                                                                                                                                                                                                                           <  1:273060/251‑1 (MQ=255)       ttACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGATCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCt                                                                                                                                                                                                                                     >  1:220465/1‑251 (MQ=255)         aCGCAGAAATTTTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATTCAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTgg                                                                                                                                                                                                                                   <  2:382725/251‑1 (MQ=255)             aGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGagag                                                                                                                                                                                                                               <  1:223137/251‑1 (MQ=255)               aaaTTGTTCCAGTAACTGTTGCTCACTCTCAAACGAAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCCGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATACCCTTCCATCGATTTACACAGTTAGTTGAGTATCAGTACGCTGGAGAGGc                                                                                                                                                                                                                             >  2:453553/1‑251 (MQ=255)                      tCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGGGtttt                                                                                                                                                                                                                      >  2:126596/1‑251 (MQ=255)                         aGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGcc                                                                                                                                                                                                                   >  2:1825/1‑251 (MQ=255)                         aGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCAAGCAAAGTGGCCCGATACAATTTAACGTGTCCGCGCAGTATGTTGGCGATACTATCGCCACCAAAATGCTGAAATTATCCAGCACTCAGCTGCCAGTTGAGGCGAGGTCGCTCGGGATGCCCTTCAATCGAATTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGCGTTTTGcc                                                                                                                                                                                                                   >  1:249489/1‑251 (MQ=255)                               tGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGATCAAAATGTCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTACATCGATTTAAACCGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTTCCtttttt                                                                                                                                                                                                             >  2:406806/1‑250 (MQ=255)                               tGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTACATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGCGAGGCGCGTTTTGCCTTTTTc                                                                                                                                                                                                             >  2:387390/1‑251 (MQ=255)                                 ttGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCAACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGAATCAGTACGCTGGAGAGGCGAGTTTTGACTTTTTCAt                                                                                                                                                                                                           >  1:366807/1‑251 (MQ=255)                                      cAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATgg                                                                                                                                                                                                      >  2:156886/1‑251 (MQ=255)                                            cAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCAGCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGc                                                                                                                                                                                                <  1:69762/251‑1 (MQ=255)                                                           ctTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCTATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAAt                                                                                                                                                                                   <  1:74714/249‑1 (MQ=255)                                                                        cAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATGTACCGTGTCCGCGCAGGTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTCTCATTATGGGTGAGCCATCGGGCGAAATTTGCCACCTGTTCCt                                                                                                                                                                    <  2:383937/251‑1 (MQ=255)                                                                             tCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACt                                                                                                                                                                <  1:503413/250‑1 (MQ=255)                                                                              cAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTAc                                                                                                                                                              <  1:397477/251‑1 (MQ=255)                                                                               aaTCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGGGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTAc                                                                                                                                                              >  1:227001/1‑250 (MQ=255)                                                                                     tttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCCTTATGGGGGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACCATGCTg                                                                                                                                                       >  2:107019/1‑251 (MQ=255)                                                                                     tttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTA‑TTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTg                                                                                                                                                       >  1:349343/1‑250 (MQ=255)                                                                                            cATCTAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTAc                                                                                                                                                                                                                                        >  1:57649/1‑163 (MQ=255)                                                                                            cATCTAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTAc                                                                                                                                                                                                                                        <  2:57649/163‑1 (MQ=255)                                                                                                gAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTTAGCGATCGGGCGAACTTTGCCACCTGTTACTCCCTACAATGCTGCAGAAAAtac                                                                                                                                             >  1:229554/1‑248 (MQ=255)                                                                                                gAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGGTTTGCCTTTTTCATTTTGGGAGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATTCTGAAGAAAATcc                                                                                                                                             >  2:302540/1‑250 (MQ=255)                                                                                                       acggCCCGATCCAATTTGCCTTGGCCGTGCAGTTTGTTGGCGATACTATCGCCCCCAAAATGCTGTA‑TTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGaa                                                                                                                                    <  2:349343/249‑1 (MQ=255)                                                                                                       aTGGCCCTATACAATTAACCTTGTCCGTGCAGTTTGTTGTCGATACTATCGCCACCAAAATGCTGTAAGTCTCCAGCAATCAGCTGCCAGTTGCGGCTATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGTCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGa                                                                                                                                     <  2:410588/251‑1 (MQ=255)                                                                                                       aTGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCCGGCGAAATTTGCCAACTGTTCCTCACTACAATGTGGAAGAAAATcccgcactt                                                                                                                                      >  2:72271/1‑243 (MQ=255)                                                                                                                   aaTTTACCGTGTCCGCGCAGTTTGTTGGCGATGCTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTGCATCGATTTAAACATTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGAGAGCAATCGGGCGAAATTTGCTAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTa                                                                                                                         <  1:453553/251‑1 (MQ=255)                                                                                                                         ccGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAAc                                                                                                                   <  1:126596/251‑1 (MQ=255)                                                                                                                               ccGCGCTGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTTCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGTCGAGTTTTGTCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGACAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCACGTAATTAAcattcat                                                                                                             <  2:450502/251‑1 (MQ=255)                                                                                                                                cgcgCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAAcattcatt                                                                                                            <  1:87539/251‑1 (MQ=255)                                                                                                                                   gcAGTTTGTTGGCGATACTATCGCCACCAAAAGTCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCGCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGAGCTGAATCATTCAGGTAATTAACATTCAttttt                                                                                                         <  1:85131/251‑1 (MQ=255)                                                                                                                                     aGTTGGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCGCCAGCAATCAGCTGCCAGTGGCGGTGAGGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTGGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAAGGCTGAAGAAAATCCAGATCTGAATCATTCACGTAATTATCATTCAGTTTTtg                                                                                                       <  2:229554/251‑1 (MQ=255)                                                                                                                                                          ccaccaATATGCTGTAATTCGCCAGCAATCAGCTGCCAGTTGCTGCGATGTTGCTCGGGATGCCCTTCCATCTATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGTCGAGTTGTGCCTTTGTCATTATGGGTGAGCAATCGTGCTAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTTTGGTTTCTATATTCTGGCGtt                                                                                  <  2:29291/251‑1 (MQ=255)                                                                                                                                                              caAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATc                                                                                                                                              >  2:473841/1‑187 (MQ=255)                                                                                                                                                              caAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATc                                                                                                                                              <  1:473841/187‑1 (MQ=255)                                                                                                                                                              caAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAgtc                                                                              <  1:48047/251‑1 (MQ=255)                                                                                                                                                              caAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAgtc                                                                              <  1:401600/251‑1 (MQ=255)                                                                                                                                                                  aTGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGtcgtcg                                                                          <  2:79666/251‑1 (MQ=255)                                                                                                                                                                  aTGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGtcgtcg                                                                          >  1:79666/1‑251 (MQ=255)                                                                                                                                                                          aTTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAAt                                                                  >  1:80548/1‑251 (MQ=255)                                                                                                                                                                                       cAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGACCTTCCATCGATTTACCCAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGcc                                                    >  2:62587/1‑251 (MQ=255)                                                                                                                                                                                         gCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCat                                                  <  1:360396/251‑1 (MQ=255)                                                                                                                                                                                         gCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCat                                                  <  1:416554/251‑1 (MQ=255)                                                                                                                                                                                                gTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGAt                                           <  1:337739/251‑1 (MQ=255)                                                                                                                                                                                                  ggcggcgATGTTGCTCGGGATTCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTTAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCGGGCGTTAGTCGTCGCCTATAATTGT‑CAGCGTGGCCATATCCGATg                                          <  2:94512/249‑1 (MQ=255)                                                                                                                                                                                                     ggcgATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTTTCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTAATTATGGGTGAGCAATCGGGCGACATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGCGGTTTCTATATTCTGGAGTTATTCGTCTCCG‑TAATTTTTCTGCTTGGCTATATCCGTTGAGtt                                      >  2:239905/1‑251 (MQ=255)                                                                                                                                                                                                        gATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCAc                                   <  1:443387/251‑1 (MQ=255)                                                                                                                                                                                                                   ggATGCCCTTCCAACGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGAccgg                        <  2:334339/251‑3 (MQ=255)                                                                                                                                                                                                                         ccTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAgg                  <  1:388152/251‑1 (MQ=255)                                                                                                                                                                                                                              cATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCa                                                                                                                                 <  2:252114/136‑1 (MQ=255)                                                                                                                                                                                                                              cATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCa                                                                                                                                 >  1:252114/1‑136 (MQ=255)                                                                                                                                                                                                                                 cGATGTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGTGAAAGTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGAttttt          <  1:366329/251‑1 (MQ=255)                                                                                                                                                                                                                                  gATTTAAACAGGTTGGTGCGTATCAGTACGCGGGAGAAGATAGTTTTGACTTTTTCATTATGGGTGAGCAATCGGGCGAAAGTTGCAAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATATGGATTATTTAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCGGGCGTTAGTCGTCGCCGATAATGTT‑CAGCGTGGCTATATCCGATGAGTTCAACGTATGACCGGAAAAGGTGATTTTTg         <  2:351129/251‑1 (MQ=255)                                                                                                                                                                                                                                         aCAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGTAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTAAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGAGTTAGTCGTCGCCGATAATTTT‑CAGCGAGGCCATATCCGGTGAGTTCACCGTATGACCGGAAAAGGGGGATTTTGAGACGcc  >  1:93889/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               CAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTT‑CAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCA  >  NC_000913/10980‑11462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑