Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 10,024 C→A G157G (GGG→GGT)  satP ← acetate/succinate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,0240CA93.0% 112.0 / ‑3.5 43G157G (GGG→GGT) satPacetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (18/22);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAG  >  NC_000913/9792‑10260
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             
ccgtgctgcagtccattgattgAAGGGCCATACGTTGAAACTGCACCATAAGTGGTTTCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAATCAATTAGTGGGA‑TTCACCACTCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCCCCCCAGATCAGCCCAATCCAGCCGGCAAAg                                                                                                                                                                                                                             <  2:156575/230‑1 (MQ=255)
      tGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGACGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGCTCAGCCCAATCCATCCGGCAACGTGgatg                                                                                                                                                                                                                       >  1:38041/1‑251 (MQ=255)
                 gCTGGAAGGGCCATACGTTGAAACTGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCAGCGCCAGATAGATTGCACTGGCACCACAGAGCAGACCAATCCAGCCGGCAAAGTGTATGATTGCGGCGt                                                                                                                                                                                                             <  1:422348/250‑1 (MQ=255)
                            cATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGt                                                                                                                                                                                                 >  1:313888/1‑251 (MQ=255)
                             aTACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGtt                                                                                                                                                                                                <  1:191727/251‑1 (MQ=255)
                                        cGGCACCAGAAGTGGTTGCTGCATGCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACATATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCca                                                                                                                                                                                     <  2:291351/251‑1 (MQ=255)
                                         ggCACCGGAAGTGGTTGCTGCATTCAGACCGCAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACAACTCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGcc                                                                                                                                                                                      >  2:214286/1‑249 (MQ=255)
                                            accagAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGTATAAAACAATCCCCCCGAGCGGGGGGATCTAAAAACAATTAGTGGGA‑TTCACAAATCGCCAGGACGTTGCGACCAAACTGCTCGTTCAGGACTTCACCCATCTCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGCCAAAGTGGATGATTGCGGCGTACCCGGCACTGTTACCGATCGCcagc                                                                                                                                                                                   >  1:38951/5‑249 (MQ=255)
                                                     ggTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAAc                                                                                                                                                                          >  2:423885/1‑249 (MQ=255)
                                                         gctgcaTTCAGCCCGAAGGGTTCAAGACTCGTCGTTAGCGAATAATAAAATCCCCCCGAGCGGGGGGATCTCAAAACAAGTAGTGGGACGTC‑CCAATCGGCAGAACGGGGCTACCAAACTGCTCGTTCAGGACTTCGCCCATCGCCAGATAGATGTCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGGGGATGATTGCGGCGTTACCGGCAATGTTCCCGATCGCCAGCAGGGCAAACAGCAc                                                                                                                                                                     <  2:259614/247‑1 (MQ=255)
                                                                        aaGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGaa                                                                                                                                                      >  1:39659/1‑250 (MQ=255)
                                                                         agagTGCAAGACGGGACGGTAGCGAATAAAAAACCCCCCCCGAGCGGGGGGATCACAAAACCATTAGTTGGA‑TTCCCCAATCGGCAGAACGGTGCGACCAAACTGCTCTTTCAGTACTTCTCCCAGCGCCAGATAGAGTGCACTGGCCCCACAGCTCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAATaaaa                                                                                                                                                    <  2:377805/251‑1 (MQ=255)
                                                                           agTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGAAAACAGCACAGTCAGGCTAAAGAAAACg                                                                                                                                                  >  2:359168/1‑251 (MQ=255)
                                                                           agTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGAGGGATCTCAACACAATTAGTGGGT‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCCGACCAATCCAGCCGGCAAAGTGGATGATTACGGCGTTACCGGCCATGTTACCGATCGCCAGCCGTGCAAACAGCCCAGTCAGGCTAAAGAAAAc                                                                                                                                                   >  1:129139/1‑250 (MQ=255)
                                                                                aaGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTg                                                                                                                                             <  1:342872/251‑1 (MQ=255)
                                                                                         cGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAAGTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAAcgcg                                                                                                                                    <  1:158091/251‑1 (MQ=255)
                                                                                           ttAGCGAGTAAAAAATTCCCCCCGAGCTGGGGGATCGCAAAACAATTTGTGGGC‑TTCACCAACCGGAAGAACGGTCCGACCAAACTGCACGTTCAGTACTCCACCCATCGCCAGATAGATTGCACTGGCACCACAGAGCAGACCAATCCAGCCGGCAAAGTGGATGAGTGCGGCGTTACCGGCAATGTTACCGAGCGCCAGCAGGTCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAAcgcgg                                                                                                                                   <  2:342341/250‑2 (MQ=255)
                                                                                                         aaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTg                                                                                                                   <  1:121847/251‑1 (MQ=255)
                                                                                                              ccccGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCgaa                                                                                                              <  1:359168/251‑1 (MQ=255)
                                                                                                                      ggggggATCTAAAAACAATGAGTGGGA‑GTCACTAAGCGGCAGAACGGTGCGATCAAACGGATAGGTCAGTACTGCACCCATCGCCAGATAGAATGCACGGGCTCCACAGATGAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTGACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAAGTGCAGAACGCGTGCGGCTTTCAG‑AGTGCCGAAGAACATaa                                                                                                      <  2:38951/251‑1 (MQ=255)
                                                                                                                       gggggATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATaa                                                                                                      >  1:415084/1‑250 (MQ=255)
                                                                                                                       gggggATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATaa                                                                                                      >  1:198434/1‑250 (MQ=255)
                                                                                                                                    aCAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAg                                                                                                                                                               <  2:404201/181‑1 (MQ=255)
                                                                                                                                    aCAATTAGTGGGA‑TTCACCAATCGGAAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAg                                                                                                                                                               >  1:404201/1‑181 (MQ=255)
                                                                                                                                      aaTTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGcc                                                                                      <  2:362781/251‑1 (MQ=255)
                                                                                                                                           gTGGGA‑TTCACCATTCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTCCCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCGAAAGAAAACGAATTGCAGAACGCGAGCGCCTTTCAG‑CGTGCCGAAGAACATACACAGCGTAAATACGCCCcac                                                                                  >  2:433393/1‑250 (MQ=255)
                                                                                                                                              ggA‑TTCACCAAGCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAAGCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGAc                                                                              <  2:313888/251‑1 (MQ=255)
                                                                                                                                                        aaTCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGt                                                                        >  2:53009/1‑248 (MQ=255)
                                                                                                                                                        aaTCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGa                                                                     <  1:53009/251‑1 (MQ=255)
                                                                                                                                                           cGGCAGAACGGTGCGCCCAAACTGCTCGTTCAGTACTTCACCCATCTCCAGATAGATTGCCCTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAACCAGCACAGTCAGGCTAGAGAACACACATTGCAGCACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCCGGTAGaccc                                                                  >  2:260812/1‑249 (MQ=255)
                                                                                                                                                                    ggTGCGACAAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACt                                                         <  1:186499/251‑1 (MQ=255)
                                                                                                                                                                              aaCTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTgg                                               <  1:246031/251‑1 (MQ=255)
                                                                                                                                                                                   cTCGTTCAGTACTTCACGCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGACAACGAATTGCAGAACGCGTGCGCCTTT‑AGTCGTGCCGAAGTACATAAACAGCGTAACTCCGCCACACAGACCCCGGTAGCCACCAAGGAAcggggcctttagcgcac                                          >  2:94721/1‑235 (MQ=255)
                                                                                                                                                                                        tCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGTGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGt                                      <  2:391422/250‑1 (MQ=255)
                                                                                                                                                                                        tCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATAAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTc                                     >  1:196256/1‑251 (MQ=255)
                                                                                                                                                                                                   cccagtgactgaaagagtgcacTGGCACCACAGATCTGACCAATCCAGCCGGCAAAGTGGAGGATTGCGGCGTTATCGGCAATGCTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACATACTCAGGTAGACACCAAGGATCTGTGCATTTGGCGCGTCTGGCAGACCCAGttt                          <  2:219464/230‑1 (MQ=255)
                                                                                                                                                                                                         gccagatagagtgaaatGGCACCACAGATAAGACAAAGCCAGCCGGCAAAGTGGATGATTGCGGCGTGACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGGGCGCCTTTCAG‑CGTGCCGAAGAACAGAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGGTTCGGCAt                    <  2:38041/235‑1 (MQ=255)
                                                                                                                                                                                                         gCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCAt                    >  1:23020/1‑251 (MQ=255)
                                                                                                                                                                                                         gCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCAt                    <  2:415084/251‑1 (MQ=255)
                                                                                                                                                                                                           cAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATca                  <  1:302250/251‑1 (MQ=255)
                                                                                                                                                                                                             gatagatTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATcagc                >  1:280108/1‑251 (MQ=255)
                                                                                                                                                                                                                        cTGGCACCACAGCTCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTCCCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGCACGCGTGCGCCTTTCAG‑CGTGCCGAAGACCATAAACAGCGTAAATACGCCCCACCGACCCAGGTAGACACCAAGGAACTGCGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCAACCGCAGAATCGCacc     >  2:170770/1‑249 (MQ=255)
                                                                                                                                                                                                                           gCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCaa  <  1:181082/251‑2 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             
CCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAG  >  NC_000913/9792‑10260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑