Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 10,015 G→A C160C (TGC→TGT)  satP ← acetate/succinate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,0150GA97.7% 125.7 / ‑4.3 44C160C (TGC→TGT) satPacetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (19/23);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

ACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAG  >  NC_000913/9770‑10260
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
ccactgtagttttGCCAGCGTACCGTACTGCATTCAGTTGCTGAAATGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCTAGCGGGGGTATCTCAAAACAATTAGTGGGA‑TTCACCAATCGTCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGa                                                                                                                                                                                                                                                    <  1:38289/241‑1 (MQ=255)
                      ccgtgctgcagtccattgattgAAGGGCCATACGTTGAAACTGCACCATAAGTGGTTTCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAATCAATTAGTGGGA‑TTCACCACTCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCCCCCCAGATCAGCCCAATCCAGCCGGCAAAg                                                                                                                                                                                                                             <  2:156575/230‑1 (MQ=255)
                            tGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGACGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGCTCAGCCCAATCCATCCGGCAACGTGgatg                                                                                                                                                                                                                       >  1:38041/1‑251 (MQ=255)
                                       gCTGGAAGGGCCATACGTTGAAACTGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCAGCGCCAGATAGATTGCACTGGCACCACAGAGCAGACCAATCCAGCCGGCAAAGTGTATGATTGCGGCGt                                                                                                                                                                                                             <  1:422348/250‑1 (MQ=255)
                                                  cATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGt                                                                                                                                                                                                 >  1:313888/1‑251 (MQ=255)
                                                   aTACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGtt                                                                                                                                                                                                <  1:191727/251‑1 (MQ=255)
                                                              cGGCACCAGAAGTGGTTGCTGCATGCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACATATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCca                                                                                                                                                                                     <  2:291351/251‑1 (MQ=255)
                                                               ggCACCGGAAGTGGTTGCTGCATTCAGACCGCAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACAACTCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGcc                                                                                                                                                                                      >  2:214286/1‑249 (MQ=255)
                                                                  accagAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGTATAAAACAATCCCCCCGAGCGGGGGGATCTAAAAACAATTAGTGGGA‑TTCACAAATCGCCAGGACGTTGCGACCAAACTGCTCGTTCAGGACTTCACCCATCTCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGCCAAAGTGGATGATTGCGGCGTACCCGGCACTGTTACCGATCGCcagc                                                                                                                                                                                   >  1:38951/5‑249 (MQ=255)
                                                                           ggTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAAc                                                                                                                                                                          >  2:423885/1‑249 (MQ=255)
                                                                               gctgcaTTCAGCCCGAAGGGTTCAAGACTCGTCGTTAGCGAATAATAAAATCCCCCCGAGCGGGGGGATCTCAAAACAAGTAGTGGGACGTC‑CCAATCGGCAGAACGGGGCTACCAAACTGCTCGTTCAGGACTTCGCCCATCGCCAGATAGATGTCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGGGGATGATTGCGGCGTTACCGGCAATGTTCCCGATCGCCAGCAGGGCAAACAGCAc                                                                                                                                                                     <  2:259614/247‑1 (MQ=255)
                                                                                              aaGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGaa                                                                                                                                                      >  1:39659/1‑250 (MQ=255)
                                                                                               agagTGCAAGACGGGACGGTAGCGAATAAAAAACCCCCCCCGAGCGGGGGGATCACAAAACCATTAGTTGGA‑TTCCCCAATCGGCAGAACGGTGCGACCAAACTGCTCTTTCAGTACTTCTCCCAGCGCCAGATAGAGTGCACTGGCCCCACAGCTCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAATaaaa                                                                                                                                                    <  2:377805/251‑1 (MQ=255)
                                                                                                 agTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGAAAACAGCACAGTCAGGCTAAAGAAAACg                                                                                                                                                  >  2:359168/1‑251 (MQ=255)
                                                                                                 agTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGAGGGATCTCAACACAATTAGTGGGT‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCCGACCAATCCAGCCGGCAAAGTGGATGATTACGGCGTTACCGGCCATGTTACCGATCGCCAGCCGTGCAAACAGCCCAGTCAGGCTAAAGAAAAc                                                                                                                                                   >  1:129139/1‑250 (MQ=255)
                                                                                                      aaGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTg                                                                                                                                             <  1:342872/251‑1 (MQ=255)
                                                                                                               cGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAAGTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAAcgcg                                                                                                                                    <  1:158091/251‑1 (MQ=255)
                                                                                                                 ttAGCGAGTAAAAAATTCCCCCCGAGCTGGGGGATCGCAAAACAATTTGTGGGC‑TTCACCAACCGGAAGAACGGTCCGACCAAACTGCACGTTCAGTACTCCACCCATCGCCAGATAGATTGCACTGGCACCACAGAGCAGACCAATCCAGCCGGCAAAGTGGATGAGTGCGGCGTTACCGGCAATGTTACCGAGCGCCAGCAGGTCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAAcgcgg                                                                                                                                   <  2:342341/250‑2 (MQ=255)
                                                                                                                               aaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTg                                                                                                                   <  1:121847/251‑1 (MQ=255)
                                                                                                                                    ccccGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCgaa                                                                                                              <  1:359168/251‑1 (MQ=255)
                                                                                                                                            ggggggATCTAAAAACAATGAGTGGGA‑GTCACTAAGCGGCAGAACGGTGCGATCAAACGGATAGGTCAGTACTGCACCCATCGCCAGATAGAATGCACGGGCTCCACAGATGAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTGACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAAGTGCAGAACGCGTGCGGCTTTCAG‑AGTGCCGAAGAACATaa                                                                                                      <  2:38951/251‑1 (MQ=255)
                                                                                                                                             gggggATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATaa                                                                                                      >  1:415084/1‑250 (MQ=255)
                                                                                                                                             gggggATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATaa                                                                                                      >  1:198434/1‑250 (MQ=255)
                                                                                                                                                          aCAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAg                                                                                                                                                               <  2:404201/181‑1 (MQ=255)
                                                                                                                                                          aCAATTAGTGGGA‑TTCACCAATCGGAAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAg                                                                                                                                                               >  1:404201/1‑181 (MQ=255)
                                                                                                                                                            aaTTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGcc                                                                                      <  2:362781/251‑1 (MQ=255)
                                                                                                                                                                 gTGGGA‑TTCACCATTCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTCCCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCGAAAGAAAACGAATTGCAGAACGCGAGCGCCTTTCAG‑CGTGCCGAAGAACATACACAGCGTAAATACGCCCcac                                                                                  >  2:433393/1‑250 (MQ=255)
                                                                                                                                                                    ggA‑TTCACCAAGCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAAGCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGAc                                                                              <  2:313888/251‑1 (MQ=255)
                                                                                                                                                                              aaTCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGt                                                                        >  2:53009/1‑248 (MQ=255)
                                                                                                                                                                              aaTCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGa                                                                     <  1:53009/251‑1 (MQ=255)
                                                                                                                                                                                 cGGCAGAACGGTGCGCCCAAACTGCTCGTTCAGTACTTCACCCATCTCCAGATAGATTGCCCTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAACCAGCACAGTCAGGCTAGAGAACACACATTGCAGCACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCCGGTAGaccc                                                                  >  2:260812/1‑249 (MQ=255)
                                                                                                                                                                                          ggTGCGACAAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACt                                                         <  1:186499/251‑1 (MQ=255)
                                                                                                                                                                                                    aaCTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTgg                                               <  1:246031/251‑1 (MQ=255)
                                                                                                                                                                                                         cTCGTTCAGTACTTCACGCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGACAACGAATTGCAGAACGCGTGCGCCTTT‑AGTCGTGCCGAAGTACATAAACAGCGTAACTCCGCCACACAGACCCCGGTAGCCACCAAGGAAcggggcctttagcgcac                                          >  2:94721/1‑235 (MQ=255)
                                                                                                                                                                                                              tCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGTGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGt                                      <  2:391422/250‑1 (MQ=255)
                                                                                                                                                                                                              tCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATAAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTc                                     >  1:196256/1‑251 (MQ=255)
                                                                                                                                                                                                                         cccagtgactgaaagagtgcacTGGCACCACAGATCTGACCAATCCAGCCGGCAAAGTGGAGGATTGCGGCGTTATCGGCAATGCTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACATACTCAGGTAGACACCAAGGATCTGTGCATTTGGCGCGTCTGGCAGACCCAGttt                          <  2:219464/230‑1 (MQ=255)
                                                                                                                                                                                                                               gccagatagagtgaaatGGCACCACAGATAAGACAAAGCCAGCCGGCAAAGTGGATGATTGCGGCGTGACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGGGCGCCTTTCAG‑CGTGCCGAAGAACAGAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGGTTCGGCAt                    <  2:38041/235‑1 (MQ=255)
                                                                                                                                                                                                                               gCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCAt                    >  1:23020/1‑251 (MQ=255)
                                                                                                                                                                                                                               gCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCAt                    <  2:415084/251‑1 (MQ=255)
                                                                                                                                                                                                                                 cAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATca                  <  1:302250/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gatagatTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATcagc                >  1:280108/1‑251 (MQ=255)
                                                                                                                                                                                                                                              cTGGCACCACAGCTCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTCCCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGCACGCGTGCGCCTTTCAG‑CGTGCCGAAGACCATAAACAGCGTAAATACGCCCCACCGACCCAGGTAGACACCAAGGAACTGCGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCAACCGCAGAATCGCacc     >  2:170770/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 gCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCaa  <  1:181082/251‑2 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
ACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGA‑TTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAG‑CGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAG  >  NC_000913/9770‑10260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑