Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,711 A→T A83A (GCT→GCA)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,7110AT96.7% 74.5 / ‑3.5 30A83A (GCT→GCA) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (7/22);  total (8/22)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

GGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGG  >  NC_000913/7465‑7956
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
ggCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCa                                                                                                                                                                                                                                                    <  1:69619/251‑1 (MQ=255)
           gaattCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGcc                                                                                                                                                                                                                                          <  1:270619/246‑1 (MQ=255)
                     gAAAATTATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCACCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGtt                                                                                                                                                                                                                                 <  1:86650/249‑1 (MQ=255)
                            agcccgtgggCGATGAGCAAAAAGACGGCGAACCGAACGCCCCTCCAGCGCATCCCCAGCCC‑GCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCATCGCAACGCCGGCCAGGTTGCCGCTAc                                                                                                                                                                                                                         <  2:203242/242‑1 (MQ=255)
                               ccgtAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                                                                                                                                                     <  1:378756/248‑1 (MQ=255)
                                    ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGGAACGCCGGCCAGGTTGCCGCTAACCAcgcgcc                                                                                                                                                                                                                >  1:374532/1‑250 (MQ=255)
                                           aGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCa                                                                                                                                                                                                           >  1:189626/1‑249 (MQ=255)
                                                 aaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGt                                                                                                                                                                                                   <  1:42738/251‑1 (MQ=255)
                                                         ccaacagaccccccaccccacgctCCCCAGCCCTGGGCGCCATCTACCAGACCGGTTCGACACGAACCGGCCCATTGGCAGCACGTTCCTTAGAATGCTGTGCGAAGGAA‑CATCTGGCAAACGAGTCCCCCCTGCCGATAAACGCGGCAACCCACAGCCAGAAGCCGGCTCCAGGCCCACCGGCGGTAACTGCCAGCGCACCGCCGGCAAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACCCaac                                                                                                                                                                                            <  2:58785/228‑2 (MQ=255)
                                                                                             gcgcAATATCCCATGCCGTTCCGCCACGAACCGGCCTATTGCAATCCCTTTCTTTAAAAAGCTGTGCGAGGGAA‑ACTCCGGCAAACGAGGCCGCCATGCCGATAAACGCGGCCACCCCCACCCAGAAGCCGGCTCCAGGACCACCGGCGGTAATTCCCAGCGCAACTCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGATTGAAATGAGGTTAAACCGCCTGGCTGTGGATGa                                                                                                                                                        <  2:121880/251‑1 (MQ=255)
                                                                                                caaattcccaggcgggccctccACAAAACTGCCCATTGACTAAACTTTCTTTATAAAGCT‑TGGGAGGGAAACTTTCGGCAAGCGAGGTCGCCATGCCGATAAACGCGGCAACCCCCATCCAGAAGACTGCTCCAGTGCCACCGGCGGGAATTGCCGGCGCAACGCGGGCCAGGTGGCTGCTGCCCACGCGCGCCGCAAGACTGGGACAGAATGCCAGAAAGGAGTTTAAACCGCCTGTCTGTGGATGAATg                                                                                                                                                     <  2:67471/231‑1 (MQ=255)
                                                                                                       ccATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCGGTGCGAGGGAC‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAGCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAAGGACTGAAATGATGTTAAACCTCCTGGCTGTGGATGAATGCTAtttt                                                                                                                                              <  1:141531/251‑1 (MQ=255)
                                                                                                               gTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCCACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTAAAAAATGACTGAAATGAGGGTTAACCGCCTGGGTGGGGGTGGATACTATTTTTAAGaatt                                                                                                                                      >  1:337019/1‑248 (MQ=255)
                                                                                                                                    tggcatCACGTTCTTTATAAAGCTGTGCGAGTGAA‑CATTCGGCAACCGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCCCCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTTTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTa                                                                                                                  <  1:146370/245‑1 (MQ=255)
                                                                                                                                                   tataAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAAAGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGGACACAATGGCTGAAATGAGGGTAAAACGCCTGGCTGTGGATGaa                                                                                                                                                       >  1:420259/1‑198 (MQ=255)
                                                                                                                                                                    gaA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATcc                                                                                                <  1:54674/236‑1 (MQ=255)
                                                                                                                                                                     aaacatccgcaaaagggggccGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCCCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGaa                                                                                <  2:189626/231‑1 (MQ=255)
                                                                                                                                                                        cATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAAACGCCTGGCTGTGGGTGAATGCTATTTTTAAGACTTTTGCCAAAATGGCGGATGTAGCGAAACTGCACAAAACCGGGGCGGAAAAG‑GAAcc                                                                              >  1:379394/1‑250 (MQ=255)
                                                                                                                                                                                    ggggcgcccttcccGATAACCGCGGCACCCCCCACCCAGAAGACGGCCCCAGGTCCCCCGGCGGTAATTTCCAGCGCAACGCCGGCCCGGTGGCCGCTACCCCCGCGCGCCGCAAGACTGTTACACAATGACTGAAATGAGTTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGcc                                                                 <  2:243338/239‑1 (MQ=255)
                                                                                                                                                                                      ggTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGGCGCTACCCAAGCGCGCCGCAAGAATGGTACACAATGAATGAAATGAGGATAAACCGCCTGGCTGTGGATGAATGCTATTTTTTAGACTTTTGCCAAACTGGCGGATGTAGCGAAAATGGACAAATCCGGGGCG‑AAAAGTGGACACACAACCTGAGCCg                                                                >  1:67579/1‑250 (MQ=255)
                                                                                                                                                                                            cATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCa                                                         <  1:199537/251‑1 (MQ=255)
                                                                                                                                                                                                        cgcgGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTa                                             <  1:376738/251‑1 (MQ=255)
                                                                                                                                                                                                               acccccacccagaagtctgCTCCAGGGCCACCGGCCGTAATTGCCAGCCGAACGCCGGCCAGGTGGCCACTACCCTCCCGCGCCGAAAGACTGGTACACAAAGAGGGAAATGAGGTTAAACCGCCTGTCTGTGGAGGAATGCTATTTTTAAGACTTTTGCCAAACGGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAGCCAACAACCTGCGCCGAAGGGCATTTAAATCAGTTCCGATcc                                      <  2:205179/233‑1 (MQ=255)
                                                                                                                                                                                                                        cagaaGACGGCTCCAGGTCCCCCGTCGTTAATTGCCAGCGCACAGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTAAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGAc                             <  1:235261/248‑1 (MQ=255)
                                                                                                                                                                                                                          gaaGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGc                           <  1:393855/250‑1 (MQ=255)
                                                                                                                                                                                                                             gACGGCTCCAGGTCCACGGGCGTGAATTGCCAGCTCAACGCCGGCCAGGGTGCCGCTACCCACGCGCGCCGCAATACTGGTACACAATGTCTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGt                        <  1:446936/251‑1 (MQ=255)
                                                                                                                                                                                                                              acgggcccAGGCCCACCGGCGGTAAGTGCACGCGCAACGCCGTCCAGGTTTCCGCGACCCCCGCGGGCCGCAAGACTGGTAAACAAGGACTTAAATGGGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCTCCGAAGAGCATGTAAATCATTACCGAGCCCCAAAGGACGCTGtt                       <  2:499982/245‑1 (MQ=255)
                                                                                                                                                                                                                                      aGGTCCACCGGCGGTAATTGCCAGAGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATTAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAgg               <  1:473451/251‑1 (MQ=255)
                                                                                                                                                                                                                                        gTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGGATGCTATTTTTAAGACTTTTGGCAAACTGGCGGATGTAGCGAAACTGCACAAAACCGGGGCG‑AAAAGAGGAACAACAAACGGAGCCGAAGAGCAAGGAAATAATTAACGAACACAAAAGGACGCTGTTAATAAAgg               >  1:97858/1‑249 (MQ=255)
                                                                                                                                                                                                                                                   gTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGACGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTgg  >  1:379016/1‑251 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
GGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGG  >  NC_000913/7465‑7956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑