Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,681 A→G F93F (TTT→TTC)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,6810AG96.4% 77.3 / ‑4.4 28F93F (TTT→TTC) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (8/19);  total (9/19)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01

CAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTA  >  NC_000913/7437‑7913
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
cAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACg                                                                                                                                                                                                                                     >  1:68946/1‑251 (MQ=255)
    agaaaaacacaggcctcgctcAACGGCGTTCGCCTGAATTCCGCTGAAAATTAAGCCGAAGGCGAGAAGCAAAAAGACGGCGAACAGAACGCCCACCCAGCGCACCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTc                                                                                                                                                                                                                                 <  2:10144/231‑1 (MQ=255)
      aaaaaCTCAGGGCTCGCGCAACGGCGTTCACCTGAATTCCGCTGAAAATTAGGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTcc                                                                                                                                                                                                                                >  1:365665/1‑250 (MQ=255)
            ccAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCa                                                                                                                                                                                                                         <  1:308279/250‑1 (MQ=255)
                 gctcgccctaCGGCGTTCGCCTGAATCCCGCTGTAAATTATGCCGGAGGCGATGAGAAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCAAGCCGGTCCGCCACGAAACTGCCCATTGTCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATACGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggc                                                                                                                                                                                                                    <  2:391944/242‑1 (MQ=255)
                            ggCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCa                                                                                                                                                                                                         <  1:69619/251‑1 (MQ=255)
                                 tCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcggacaatgccagccc                                                                                                                                                                                                     >  2:68543/1‑237 (MQ=255)
                                       gaattCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGcc                                                                                                                                                                                               <  1:270619/246‑1 (MQ=255)
                                                 gAAAATTATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCACCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGtt                                                                                                                                                                                      <  1:86650/249‑1 (MQ=255)
                                                        agcccgtgggCGATGAGCAAAAAGACGGCGAACCGAACGCCCCTCCAGCGCATCCCCAGCCC‑GCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCATCGCAACGCCGGCCAGGTTGCCGCTAc                                                                                                                                                                              <  2:203242/242‑1 (MQ=255)
                                                           ccgtAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                                                                                                          <  1:378756/248‑1 (MQ=255)
                                                                ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGGAACGCCGGCCAGGTTGCCGCTAACCAcgcgcc                                                                                                                                                                     >  1:374532/1‑250 (MQ=255)
                                                                       aGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCa                                                                                                                                                                >  1:189626/1‑249 (MQ=255)
                                                                             aaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGt                                                                                                                                                        <  1:42738/251‑1 (MQ=255)
                                                                                     ccaacagaccccccaccccacgctCCCCAGCCCTGGGCGCCATCTACCAGACCGGTTCGACACGAACCGGCCCATTGGCAGCACGTTCCTTAGAATGCTGTGCGAAGGAA‑CATCTGGCAAACGAGTCCCCCCTGCCGATAAACGCGGCAACCCACAGCCAGAAGCCGGCTCCAGGCCCACCGGCGGTAACTGCCAGCGCACCGCCGGCAAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACCCaac                                                                                                                                                 <  2:58785/228‑2 (MQ=255)
                                                                                                                         gcgcAATATCCCATGCCGTTCCGCCACGAACCGGCCTATTGCAATCCCTTTCTTTAAAAAGCTGTGCGAGGGAA‑ACTCCGGCAAACGAGGCCGCCATGCCGATAAACGCGGCCACCCCCACCCAGAAGCCGGCTCCAGGACCACCGGCGGTAATTCCCAGCGCAACTCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGATTGAAATGAGGTTAAACCGCCTGGCTGTGGATGa                                                                                                             <  2:121880/251‑1 (MQ=255)
                                                                                                                            caaattcccaggcgggccctccACAAAACTGCCCATTGACTAAACTTTCTTTATAAAGCT‑TGGGAGGGAAACTTTCGGCAAGCGAGGTCGCCATGCCGATAAACGCGGCAACCCCCATCCAGAAGACTGCTCCAGTGCCACCGGCGGGAATTGCCGGCGCAACGCGGGCCAGGTGGCTGCTGCCCACGCGCGCCGCAAGACTGGGACAGAATGCCAGAAAGGAGTTTAAACCGCCTGTCTGTGGATGAATg                                                                                                          <  2:67471/231‑1 (MQ=255)
                                                                                                                                   ccATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCGGTGCGAGGGAC‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAGCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAAGGACTGAAATGATGTTAAACCTCCTGGCTGTGGATGAATGCTAtttt                                                                                                   <  1:141531/251‑1 (MQ=255)
                                                                                                                                           gTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCCACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTAAAAAATGACTGAAATGAGGGTTAACCGCCTGGGTGGGGGTGGATACTATTTTTAAGaatt                                                                                           >  1:337019/1‑248 (MQ=255)
                                                                                                                                                                tggcatCACGTTCTTTATAAAGCTGTGCGAGTGAA‑CATTCGGCAACCGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCCCCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTTTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTa                                                                       <  1:146370/245‑1 (MQ=255)
                                                                                                                                                                               tataAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAAAGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGGACACAATGGCTGAAATGAGGGTAAAACGCCTGGCTGTGGATGaa                                                                                                            >  1:420259/1‑198 (MQ=255)
                                                                                                                                                                                                gaA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATcc                                                     <  1:54674/236‑1 (MQ=255)
                                                                                                                                                                                                 aaacatccgcaaaagggggccGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCCCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGaa                                     <  2:189626/231‑1 (MQ=255)
                                                                                                                                                                                                    cATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAAACGCCTGGCTGTGGGTGAATGCTATTTTTAAGACTTTTGCCAAAATGGCGGATGTAGCGAAACTGCACAAAACCGGGGCGGAAAAG‑GAAcc                                   >  1:379394/1‑250 (MQ=255)
                                                                                                                                                                                                                ggggcgcccttcccGATAACCGCGGCACCCCCCACCCAGAAGACGGCCCCAGGTCCCCCGGCGGTAATTTCCAGCGCAACGCCGGCCCGGTGGCCGCTACCCCCGCGCGCCGCAAGACTGTTACACAATGACTGAAATGAGTTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGcc                      <  2:243338/239‑1 (MQ=255)
                                                                                                                                                                                                                  ggTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGGCGCTACCCAAGCGCGCCGCAAGAATGGTACACAATGAATGAAATGAGGATAAACCGCCTGGCTGTGGATGAATGCTATTTTTTAGACTTTTGCCAAACTGGCGGATGTAGCGAAAATGGACAAATCCGGGGCG‑AAAAGTGGACACACAACCTGAGCCg                     >  1:67579/1‑250 (MQ=255)
                                                                                                                                                                                                                        cATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCa              <  1:199537/251‑1 (MQ=255)
                                                                                                                                                                                                                                    cgcgGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTa  <  1:376738/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
CAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTA  >  NC_000913/7437‑7913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑