Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,365,526	IS2 (–) +5 bp	coding (1244‑1248/1302 nt)	dcuA ←	C4‑dicarboxylate transporter DcuA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	49 (1.030)	45/486	0.1	90.6%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	4365526 =	5 (0.110)					coding (1248/1302 nt)	dcuA	C4‑dicarboxylate transporter DcuA
*	?	NC_000913	= 3187426	NA (NA)	67 (1.410)	60/484	0.0	94.2%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	= 4365530	4 (0.090)					coding (1244/1302 nt)	dcuA	C4‑dicarboxylate transporter DcuA

GCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGG  >  NC_000913/3187181‑3187671                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGtgg                                                                                                                                                                                                                                                    <  1:322945/250‑3 (MQ=11)  cGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTatc                                                                                                                                                                                                                                                  >  1:380640/1‑251 (MQ=31)      gTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTAtcatca                                                                                                                                                                                                                                              <  1:387945/251‑1 (MQ=33)            agagTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGGGCGCTGGGTTATCGCTCGCCACGGGCATATCTGCGGCAGCGGGCTTGTAATGGGGTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGtt                                                                                                                                                                                                                                        >  2:394837/1‑251 (MQ=255)              agTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTtgt                                                                                                                                                                                                                                      <  1:417822/251‑1 (MQ=37)               gTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTtgtg                                                                                                                                                                                                                                     >  2:26900/1‑251 (MQ=38)                   cGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTc                                                                                                                                                                                                                                 >  1:17591/1‑251 (MQ=39)                    ggAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGAATCATGCCCCAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCACAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGACTATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTc                                                                                                                                                                                                                                 >  1:66707/1‑250 (MQ=255)                         aGCAGAGAGCTTCGTGAAAACTATAAAGCGTGAATACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTGCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTa                                                                                                                                                                                                                           <  2:454609/251‑1 (MQ=255)                          gCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTAt                                                                                                                                                                                                                          >  1:180345/1‑251 (MQ=255)                            agagagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGc                                                                                                                                                                                                                        <  1:170599/251‑1 (MQ=255)                                agCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTTCGTCAGCTTATGCCGtt                                                                                                                                                                                                                    <  1:394837/251‑1 (MQ=255)                                 gCTTCGTTAAAACGTAAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGTGTATCGCTCGCCCCGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTg                                                                                                                                                                                                                   <  2:271524/251‑1 (MQ=255)                                      gTGAAAACGATAAAGCGTGACTACATCAGGATCCTGCCCAAACCAGACGGGTTAAAGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGTCAGCGGGCGTGTAATGGGGTCAGTGATAACAGATGTCTGGACATTTAGGGGCACATCCAGTTCTCTTCATTGGTTGTGCGTCAGCTTATGcctttacattc                                                                                                                                                                                                              >  2:114243/1‑242 (MQ=14)                                       tGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGc                                                                                                                                                                                                                        <  1:371691/240‑1 (MQ=255)                                       tGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGc                                                                                                                                                                                                                        >  2:371691/1‑240 (MQ=255)                                        gAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAACCCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCGCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTcaa                                                                                                                                                                                                            <  1:304662/251‑1 (MQ=255)                                        gAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTcaa                                                                                                                                                                                                            <  1:48962/251‑1 (MQ=255)                                                      gTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGCGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGATTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg                                                                                                                                                                                              >  1:506188/1‑251 (MQ=255)                                                       tGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg                                                                                                                                                                                              >  2:205912/1‑250 (MQ=255)                                                        gcatACATCATTATCATGCCCATACCAGTCGGGTTAACTGCAGCAAAGAACCGTGCAGAGGCGTTCGAGTATTATAACGAATGGCATCCGCATAGTGCTCTGGGTTATCGCTCGCCACGGGAATATCTGCGTCAGCGGGCTTGTAATGTGGTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTAACATCATTGGTTTTGCGTCAGCTTATGCCGTTGAATTCAACTAAGATTTCATCGaa                                                                                                                                                                                            <  2:297180/248‑1 (MQ=17)                                                        gactaaagcaggaTCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGaa                                                                                                                                                                                            <  2:403007/239‑1 (MQ=255)                                                         aCTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGGGATAACTGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGa                                                                                                                                                                                             >  2:378361/1‑249 (MQ=255)                                                          cTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCACAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGc                                                                                                                                                                                          >  1:391886/1‑251 (MQ=255)                                                            aCATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAAGTCAACAAAGATTTAATCGAAGCCg                                                                                                                                                                                        <  2:305590/251‑1 (MQ=255)                                                             cATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCg                                                                                                                                                                                        >  1:60330/1‑250 (MQ=255)                                                                cAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAAACTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAg                                                                                                                                                                                     <  2:71948/250‑1 (MQ=255)                                                                 aGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑t                                                                                                                                                                                  <  1:385003/251‑1 (MQ=255)                                                                  gTATCCTGCCCAAACCAGACGGGTTAACGGCATCAAAGAACCTGGCAGAGGAGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTGATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCAGCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑Tc                                                                                                                                                                                 <  2:198319/251‑1 (MQ=255)                                                                  gTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑Tc                                                                                                                                                                                 <  1:114745/251‑1 (MQ=255)                                                                   tATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGa                                                                                                                                                                                       >  2:175455/1‑245 (MQ=255)                                                                            cAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGt                                                                                                                                                                       >  2:247968/1‑251 (MQ=255)                                                                             aaaCCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGtt                                                                                                                                                                      >  2:230977/1‑251 (MQ=255)                                                                              aaCCAGACGGGTGAACGGCAGCAAAGACCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTa                                                                                                                                                                     <  1:277394/251‑1 (MQ=255)                                                                                 cAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAAcc                                                                                                                                                                  <  1:470716/251‑1 (MQ=255)                                                                                    aCGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCCTTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATACCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTATCCttt                                                                                                                                                               <  1:506268/251‑1 (MQ=255)                                                                                     cGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTc                                                                                                                                                              <  1:26900/251‑1 (MQ=255)                                                                                       ggTTAACGGCAGCAGAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCAGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGCAGA‑ACGGGAAAAAGTTAACGTTTccc                                                                                                                                                            >  2:368498/1‑251 (MQ=18)                                                                                       ggTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCa                                                                                                                                                                                                                                >  1:466879/1‑184 (MQ=255)                                                                                       ggTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCGGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATATGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCa                                                                                                                                                                                                                                <  2:466879/184‑1 (MQ=255)                                                                                        gTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCTAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCTACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCa                                                                                                                                                           <  1:336175/251‑1 (MQ=255)                                                                                                         aCCTTGCAGAGGCGTTCGCGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGcc                                                                                                                                          >  1:348925/1‑251 (MQ=255)                                                                                                                  gggCGGTCGAGCATTATAGCGAAGTGCTTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCATCGGGCTTGTAATGGGTTAAGTGATAATAGATGTCTGGAAATATAGGGGCAAAGCCAGTTAGCATCAGTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCg                                                                                                                                 <  1:457663/250‑1 (MQ=25)                                                                                                                           ggCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAat                                                                                                                        <  2:380640/250‑1 (MQ=255)                                                                                                                           aGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTCTCGCTCGCCACGGGAATATCTGCGGCAGATGGCTTGTAATGGGGTAAGTGATAACAGATGTCTGGAAATATAGGTGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGACAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAat                                                                                                                        <  2:189871/251‑1 (MQ=255)                                                                                                                           aGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAat                                                                                                                        >  1:98717/1‑251 (MQ=255)                                                                                                                                 ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGACAATTACCCGTCGGCAAATATTCAc                                                                                                                  >  2:198226/1‑251 (MQ=255)                                                                                                                                               ccGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACGGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGAtt                                                                                                    <  2:438937/251‑1 (MQ=255)                                                                                                                                                cgcagtgTGCGCTGGGTTAGCGCTCGCTACGGGAATATCTGCGGCAGCGGGCTTGTAATTGGTTAAATGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCTTCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTTAATCTAATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATta                                                                                                   <  2:417542/245‑1 (MQ=37)                                                                                                                                                 gCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGACGGCAAATATTCACTAAGCACTCTGATta                                                                                                   >  1:204122/1‑250 (MQ=255)                                                                                                                                                   aTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaat                                                                                                 >  2:41584/1‑250 (MQ=255)                                                                                                                                                     aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAAGGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGCATTCAACAAAGATTTAATCGAAGCCGCAGA‑TCGTGAAACCGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGccccctgcttactaat                                                                                              >  2:232496/1‑236 (MQ=37)                                                                                                                                                             gggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAGCTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGAc                                                                                      <  2:180345/251‑1 (MQ=255)                                                                                                                                                                              gggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGAcc                                                                     <  1:428471/251‑1 (MQ=255)                                                                                                                                                                                        gCGGCAGCGGGCTTGTAATTGGTTAAGTGATAACAGATGTCGGGAAATATAGGGGCAAATCCAGTTATCAGCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGt                                                           <  2:506188/251‑1 (MQ=255)                                                                                                                                                                                           gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTAtt                                                        <  1:247968/251‑1 (MQ=255)                                                                                                                                                                                                   cTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCAAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGAt                                                 >  2:212350/1‑250 (MQ=255)                                                                                                                                                                                                           gggTTAAGTGATAACAGATTTCTGGAAATAGAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACt                                        <  1:249051/251‑1 (MQ=255)                                                                                                                                                                                                           gggTTAAGTGATAACAGAGGTCTTGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGGGCGTCAGTTTATGCCGATGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAGACGGTAACCTTTCCCAATGTGAAACGGATGGCCAATTACCCGTCGGTAAATAGTCCCTAAGCTCTCTGATTATTAATAAGATGACGCCGATCCTCCTTGACCGCCAAGGGGTATTCATTGATTACCAAAcc                                        <  2:92812/251‑2 (MQ=255)                                                                                                                                                                                                            ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTg                                       >  2:120415/1‑251 (MQ=255)                                                                                                                                                                                                                            aTGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGACAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACAcc                       <  1:198226/251‑1 (MQ=255)                                                                                                                                                                                                                                  ggAAATATAGGGGCAAATTCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCagag                   <  1:168414/249‑1 (MQ=255)                                                                                                                                                                                                                                   gAAATATAGGGGCAAATCCAGTTATCATCATTGGTTATGCGTCAGCTTATGCCGTTGAATTCACCAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGGCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGCATTAATTGATACCCAACCTGCTGATTGCGTTACACCAGAGCa                 >  2:186761/1‑250 (MQ=255)                                                                                                                                                                                                                                     aaTATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAAtt              <  1:230977/251‑1 (MQ=255)                                                                                                                                                                                                                                        ataGGGGCAAATTCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTaa            <  1:477506/250‑1 (MQ=255)                                                                                                                                                                                                                                         tatgtgccaaTCCAGTTATCCTCATTGGTTGTGAGTCAGCTTAAGACGTTTAATTTAACAAAGATTTAACAGATGCCGAAGAATC‑TGAACACGTTACCCTTCACTAATTAGACACGTATGGCCAATTACCCTTCTGCACATATTCACTATGTCCTCTGATTAATAATAAGAGGACGCCAATCCCCCTTGACCTCCAATGGGTATTAATGGATCACCAAACTGCAGTTTGCGTGACGCCCGAGCCATTAAc           <  2:128486/242‑1 (MQ=255)                                                                                                                                                                                                                                          aGGGGCAAATCCAATTATCATCATTGGTTGGGCGTCAGCTTATGCCGTTTAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTATCat         <  2:391886/251‑1 (MQ=255)                                                                                                                                                                                                                                               cAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAATAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACAttatta    <  1:273444/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aTCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAgg  >  1:84402/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGA‑TCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGG  >  NC_000913/3187181‑3187671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.