Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	362,204	IS1 (–) +9 bp	coding (968‑976/1254 nt)	lacY ←	lactose permease

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	19796 =	NA (NA)	46 (0.970)	43/486	0.2	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 362212	0 (0.000)					coding (968/1254 nt)	lacY	lactose permease
*	?	NC_000913	= 258675	NA (NA)	49 (1.030)	47/486	0.1	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	362204 =	0 (0.000)					coding (976/1254 nt)	lacY	lactose permease

ACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAG  >  NC_000913/258430‑258680                                                                                                                                                                                                                                                      |      ccgcaatgcattgCCATATAAATGATTTTCTGGAGTGTACCGGGTGGAGATGCGGGGTAAGTGATCTGCAGTTGTCATTTTTTACGGCATGGATAGCAGAGATAGCGCTTATGTCCGGCGGTGCTTTTGCCTTTACGCCCCACCCCGTCAGTAGCTGAACAGGAGGCACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCCCCTgcgt  <  2:285726/239‑4 (MQ=11)                                                                                                                                                                                                                                                      |      ACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAG  >  NC_000913/258430‑258680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.