Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,542,692 +A intergenic (+59/‑423) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,542,6921.A85.7% 26.7 / ‑0.8 14intergenic (+59/‑423)fimE/fimAregulator for fimA/type 1 fimbriae major subunit
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base A (7/5);  total (8/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTATCTCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAA‑C‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATA  >  NC_000913/4542447‑4542942
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                           
gTTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTATCTCGGGCATCGAAATATTCGCCCTACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTACAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAAAAAATACAAGACAAT‑GGGGCCAAAAC‑TGTc                                                                                                                                                                                                                                                       >  2:36957/1‑251 (MQ=255)
     ggCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTATCTCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAAAC‑TGTCCatat                                                                                                                                                                                                                                                  >  2:426549/1‑251 (MQ=255)
              gcagTTATGATGTGGCGGAGCGATGTACAGATACTCGTTTAATTCAGGTATACCGCGGGAATCGAAATATTCGCTATACTGTGCTTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATGGATAATAAAGTTAAAAAAACAAATAAATACAAGTCAATTGGGGCCAAA‑T‑TGTCCATATCataaataa                                                                                                                                                                                                                                         <  1:135975/248‑1 (MQ=255)
                                              aCTCGTTTAATTCAGGATTATCTCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATAtt                                                                                                                                                                                                         >  2:307908/1‑251 (MQ=255)
                                                           aGGATTATCTCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAA‑AATGTCCATATCATAAATAAGTTACGTATTTTTTCTCAATCATAAAAATATTAAAAAAcaacaa                                                                                                                                                                                             >  2:343119/1‑250 (MQ=255)
                                                                tATCTCGGGCATCGAAATATTCGCCAAACTGTGCGTTATACCGACAGTAGTGCTGATCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAAAC‑TGTCCATATCATAAATAAGTTAAGAATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAGAGc                                                                                                                                                                                        >  2:326007/1‑250 (MQ=255)
                                                                   ctcGGGCATCGACATATTCGCCCTACTGTGCGTTATACCGCAAGTAATGCTGCTCGTTTTGCCGGATTATGGGACAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATACTAAAGATAAAAAAACCAATAAATACAAGACAAT‑GGGGCCAAAAC‑TGCCCATATCATAAATAAGTTCCGTTTTTTTTCTCAATCTTAAACATATTAAAAAACGACAAAAAGCATc                                                                                                                                                                                     >  2:13146/1‑250 (MQ=255)
                                                                                                   ttATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGt                                                                                                                                                     >  1:39563/1‑250 (MQ=255)
                                                                                                     ataCCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTaaa                                                                                                                                                  <  1:307908/251‑1 (MQ=255)
                                                                                                                      gctCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCt                                                                                                                                 <  1:104122/251‑1 (MQ=255)
                                                                                                                                                                gAAAAATTAAAAAGAGAAGAGGGTTGATGTAACTTATTGATAAGAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCAAAAAC‑TGTCCAGATCATAAATAAGTTACGTATTTTGTCTCAAGCATAAAAATATTAAAAAACGATAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCGCCGCTATATGTAAAGCTAACGTTTCTGTTGCTTGACGCATCt                                                                                       <  2:260884/251‑1 (MQ=255)
                                                                                                                                                                       tAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAAAC‑TGTCCATATCATAAATAAGTTACGTCTTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGt                                                                                                                      >  1:320627/1‑213 (MQ=255)
                                                                                                                                                                       tAAAAAGAGAAGAGGATTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATGTCACATCACCTCCGCtataggt                                                                                                                      <  2:320627/213‑4 (MQ=255)
                                                                                                                                                                                     gTTTGATTTAACGTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCCAAAAC‑TTTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTTTAAATTAATCTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATGCTTCTCTCCaaaa                                                                  <  1:469575/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    aa‑C‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACaaa    >  2:151474/1‑250 (MQ=255)
                                                                                                                                                                                                                                                     a‑C‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGTCAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCGCGACGCATCTTCCTCATTCTTCGCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATa  <  2:48112/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                           
GTTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTATCTCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAA‑C‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATA  >  NC_000913/4542447‑4542942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑