| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,492,583 | (G)6→7 | intergenic (‑58/+4) | idnT ← / ← idnO | L‑idonate/5‑ketogluconate/gluconate transporter/5‑keto‑D‑gluconate 5‑reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,492,583 | 1 | . | G | 88.2% | 45.3 / 0.0 | 17 | intergenic (‑58/+4) | idnT/idnO | L‑idonate/5‑ketogluconate/gluconate transporter/5‑keto‑D‑gluconate 5‑reductase |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (9/6); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGG‑CTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGT > NC_000913/4492337‑4492816 | cATTGCCGGCCCGACGGGGGTGCTGCCGATTCAAGTGTGTATAGAGTGCAGGACGGCCTGTGCATCCAACCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑gggggggct < 2:328255/250‑4 (MQ=255) ttgccaccccgcagcgagtactgacGATACCTTGTTGTATAGAGATCAGGACGGCCTGTGAACCAATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCTTTATCTTTAAAGCCGATCATCAGGATAAGAAGCATCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGTGGG‑CTGtt < 2:155394/226‑1 (MQ=255) aGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAAc > 2:84439/1‑250 (MQ=255) ccgAGCGTGCTGCCGATACCATTTTGAATAGAGTGCAGGACGCCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGACAGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGACTGTATCTCTTATTAGGGCCAGAAAAGCAGCGCTTTCGCGCTGAGCGTA‑ggggggggtgtgaaacagacact > 2:144674/1‑234 (MQ=255) tGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTACCATGCCGCCATCAACAaacaac > 2:192495/1‑251 (MQ=255) cAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGtt > 1:210547/1‑251 (MQ=255) tgtgCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGAAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACTCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATc < 1:473351/250‑1 (MQ=255) aaaTCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGa > 1:393765/1‑250 (MQ=255) atcccacaacgGCAGCCCCCAGAACGAGTGCAAAAAAGCCGTGAACTTTAAAGACGATAATCAGGATAAGAAGCAGGGCTGCGCCTTCCGCAATAATGAGTAAGGGTATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAACCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGTAAGGcaca < 2:54676/243‑1 (MQ=255) aCCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGt > 2:180806/1‑251 (MQ=255) aaCGAGGGCAATAAAGCCGTTATCTTTAAAACAGAACATCAGGAAAAAAAGCAGCGCGCCGCCTGCCTCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTATGGGGGG‑CTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTg < 1:279154/251‑1 (MQ=255) cAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATACGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGCACACCGCAGCACCACTCAGTTCCTGCGGAtctc > 1:278002/1‑251 (MQ=255) aagacGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTCACATGCCGCCATCAACAAACAACAGGTGGCCGCTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGCTCTCCCCCGCGTGCGGCGGGGGTc > 1:319048/5‑251 (MQ=255) atcaGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGcac < 1:82513/251‑1 (MQ=255) tGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCCCAACCAGGCGGGGaacggaagagcggc > 2:379730/1‑239 (MQ=255) tGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCgg < 1:379730/235‑1 (MQ=255) ataatGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAACTCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGACCGGTTGCACCACCAAGCggggcagggatcctacccaccccgg > 2:486430/1‑228 (MQ=255) atGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCt < 1:84439/251‑1 (MQ=255) cgcTGCACGTA‑GGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATtgt < 1:154618/251‑1 (MQ=255) | CATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGG‑CTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGT > NC_000913/4492337‑4492816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |