Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 5,067 Δ1 bp intergenic (+47/‑167) thrC → / → yaaX threonine synthase/DUF2502 domain‑containing protein YaaX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009135,0670C.84.6% 72.7 / 14.1 26intergenic (+47/‑167)thrC/yaaXthreonine synthase/DUF2502 domain‑containing protein YaaX
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base . (13/9);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.54e-01

CGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTT  >  NC_000913/4855‑5316
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
cGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAggg                                                                                                                                                                                                                    >  1:294053/1‑251 (MQ=255)
     ccGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGaaaa                                                                                                                                                                                                                >  1:444036/1‑250 (MQ=255)
      cgcacatgcGGCGAAATTAAAAGAGAGCGTGGAAGCGATTCCCGTTGAAACTT‑TGGATCTGCAAAAAGAGCTGGCAGATCGTGCTGATTTACCCTTGCTTTACCATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAg                                                                                                                                                                                                              <  2:444036/243‑1 (MQ=255)
                     aTTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAAt                                                                                                                                                                                                >  1:135412/1‑250 (MQ=255)
                        taaaaagcgtgTGGAATCGATTCTCGGT‑AAACCTATGGATCTGCAAAAAGAGCTGTCAGATCGGGCGTATTAACCATCTCTTTCACATAA‑CTCCCCCGCCTATTTTGCTTCTGTGCGTAAATTGATGATGAATCATCTGTAAAATCTATTCATTATCTCAATCAGGCCGGATTTGCTTTTATGCAGCT‑TGCTTTTTTATTAAGAAATGATGGAGAATAACGACAGGGAAATAGTATAAAGTCACAATaaa                                                                                                                                                                                             <  2:74499/240‑1 (MQ=255)
                                          gATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGAtt                                                                                                                                                                           >  2:437126/1‑250 (MQ=255)
                                           attctcgggtaataagtggtaTCTACCAAAAGAGCGGGCAGAACTGGCTGATTGCCCTTTGTTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCTTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCGTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTACCTTAGAGATTa                                                                                                                                                                          <  2:499323/231‑1 (MQ=255)
                                                   tGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCAGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCgg                                                                                                                                                                 >  1:50563/1‑251 (MQ=255)
                                                   tGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGAAAAATCTATTCATTATATCAATCAGGCCGAGTTTGATTTTATGCAGCC‑GGCTTTTTTATAAAGAAATTATGGAGAAAAACGACAGGGAAACAGGAGAAATTCTCAATAACTGCGGTAACTTAGAGATTAGGATTGCgg                                                                                                                                                                 >  1:286578/1‑251 (MQ=255)
                                                       aacgtgggaacggCCAAAAGAGTGGGCAGAACGGTCTGATTTCCCTTTGCTTTCACATATTCGGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGgaga                                                                                                                                                              <  1:22939/239‑1 (MQ=255)
                                                              gATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATaa                                                                                                                                                                                              >  1:252684/1‑213 (MQ=255)
                                                                tCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAAcc                                                                                                                                                     >  1:168474/1‑251 (MQ=255)
                                                                     aaaaaGAGCTGGCAGAACGTGTTTATTTACCCTTGCTTTAACATAATCTGCCC‑GCCGATTTTGCTGCGGTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACcgtcgt                                                                                                                                                <  1:373106/250‑1 (MQ=255)
                                                                       aaatagcgggCAGACCGTGCTGACTTCCCCTTTCTTACATATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTtc                                                                                                                                              <  2:447430/243‑1 (MQ=255)
                                                                           agCTGGCAGCACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACCGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTACGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCAt                                                                                                                                          >  2:311122/1‑251 (MQ=255)
                                                                                                     tggattctattaatgcttccc‑GCCGATTTTGCTGTGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGACGGGTTTGCTTTTATGCAGCC‑GGCTTGTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCa                                                                                                                 <  1:45265/232‑1 (MQ=255)
                                                                                                       gCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGAAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATa                                                                                                               >  1:347978/1‑250 (MQ=255)
                                                                                                                         cctccgATGTTGCTGCGTTGCGTAAAATGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCA‑TCCGGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGCTaaacgg                                                                                             <  2:96154/248‑4 (MQ=255)
                                                                                                                             cgATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                                                          >  2:363565/1‑250 (MQ=255)
                                                                                                                                                        gatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAAAGACAGGGAAAAAGGAGACATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGACGATCTCATCGCGTAATCTCCGGATATCGAACCATAACGGGCAATGATAAAAGGAGGAACCTATGAAAAAGATGCAATCTAt                                                                 >  1:346194/1‑248 (MQ=255)
                                                                                                                                                           gaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAAt                                                                                                                                                                                                       <  2:210404/112‑1 (MQ=255)
                                                                                                                                                           gaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAAt                                                                                                                                                                                                       >  1:210404/1‑112 (MQ=255)
                                                                                                                                                           gaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTAc                                                            <  1:434251/250‑1 (MQ=255)
                                                                                                                                                                             aTTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGTAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGt                                            <  2:294053/248‑1 (MQ=255)
                                                                                                                                                                                               gCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGGGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGAGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCAATAACGGGCAATGATAAAAGGAGTAGCCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCACATGgcagc                       >  1:1871/1‑251 (MQ=255)
                                                                                                                                                                                                   ggTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGcac                     >  2:74773/1‑249 (MQ=255)
                                                                                                                                                                                                                     ccGGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGtt  <  2:79790/251‑1 (MQ=255)
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
CGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTT  >  NC_000913/4855‑5316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑