Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	310	(T)9→8	intergenic (+55/‑27)	thrL → / → thrA	thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	310	0	T	.	96.5%	105.6 / ‑4.1	29	intergenic (+55/‑27)	thrL/thrA	thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (14/14);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAAC  >  NC_000913/67‑556                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       cagcTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCaaa                                                                                                                                                                                                                                                <  2:36493/251‑1 (MQ=255)       cgcaaCTGTTTCCCTGCAGTGAGTAAATTAAAATTTTATTGAATTAGGTCACTAAATCCTTTAACCAATAAAGGCATAGCGCACAGACAGATAAAAAGTACAGAGAACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCGCCTGACAGTGCGGGCGTTTTTTT‑CGCCCAAAGGTaa                                                                                                                                                                                                                                           <  2:51908/247‑1 (MQ=255)              ggTTACCTGCCGTGAGTCAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATCTAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGCACAAAGGTAACGAggaa                                                                                                                                                                                                                                    >  2:154531/1‑248 (MQ=255)                  aCCTGCCGTAAGTAAAATAAAAGTTTATTGTATTTGGTGACAAAATACTGAAACCAAAATAGGCATAGCACACCAAAACATAATAATTACCGACAACACAAAAAAACTGATAAGCATTTGCACAACTATTACCACCCCCACCACCATTCCCACAGGTAACGGTGCTGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTGTTA‑CGACCAAAGGTAACGAGGTcacaac                                                                                                                                                                                                                               <  2:263225/251‑1 (MQ=255)                                      acttttATTGACTTAGGTCACTAAATACTTTAACCAATATAGACCTAGCGCACCGCCAGATAACAATTACAGCGTAAACAACATCCATGAAACGCAATAGCACCACCATTACCACCACCATAACCATTACCACAGGTAACGGTGCGGACTGACGCGTACAGGAAACACAGAAACACGACCGCGCCTGACAGAGCGGACTTTTTTTT‑CGACCAAAGGAAACGAGAAAACAACCATGCGAGTGTTCAATTTcg                                                                                                                                                                                                           >  2:273224/3‑251 (MQ=255)                                               gACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTTCCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACa                                                                                                                                                                                                   <  1:308384/250‑1 (MQ=255)                                                        cACTAAATACTCTAACCAATATAGCAATAGCGAACACACAGATAAAACTAACAGAATACACAACATCCATGCAACGCCTTAGCACCACCCTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCAGACGCGTACAGGCAACCCAGAAAAAAGCCCGCACCTGACCGTGCGGGCATTTTTTT‑AGCCCCAAGAACACGAGGTACCAACCATGCGAGTGTTGAAGTTCGGCGGTGCATCCGTGGCa                                                                                                                                                                                          >  2:293461/1‑250 (MQ=255)                                                              aTACTTTAACCAATATAGGCATATCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCGCCACCATTGCCGCCACCATCACCAATACCAAATGTAACGGGGCGGGCTGACGGGTACAGGAAACACAAAAAAAAGCCCGCACCTGACAGTGCAGG‑GTGTTTTTTCGACCAAAGGTAACGAGGGAACAACCAATCGAGTGTTGAAGTTCGGCGTGACAACAGTGACAAATGCAg                                                                                                                                                                                   <  2:498161/251‑1 (MQ=255)                                                                  tttAAACAATATAGGCATAGCGCACAGACAGATAAAAATTACAGGGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACAACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCAGCACCTGCCAGTGCGGGCTTTTTTTT‑CGACCAAAGGTGACGAGGTACAAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAATGGCCAATGCAGAACg                                                                                                                                                                               >  2:455314/1‑251 (MQ=255)                                                                     aaCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACCACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt                                                                                                                                                                            >  1:52090/1‑251 (MQ=255)                                                                               ggCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAAGTTTTCTGCGGGtt                                                                                                                                                                  >  1:296474/1‑251 (MQ=255)                                                                                    aGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGGTTTCTGCGGGTTGCCg                                                                                                                                                              >  1:176071/1‑250 (MQ=255)                                                                                         gcagacagaTAACAATTACAGAGTACACAACATCCATGAAACACATTAGCACCAACATTACCACCACAATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACCCAGAAAAAAGCCAGCACCTGACGGTGCGGGCTTTTTTT‑‑CGACCAAAGGTAACAAGGAACCAAGCATGCGTGAGTTGAAGTTCGGCGGTACATCAGTGGCAAATGAAGAACGTTTTCTGCGGGTTggctctattct                                                                                                                                                       >  2:39779/2‑241 (MQ=255)                                                                                           agaaaaaTAAAAATTACAGAACACACAACTACCAGGAAACACATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCAGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGACCGTTTTCTGCGGGTTGCCGATATTCTg                                                                                                                                                      <  2:244405/245‑1 (MQ=255)                                                                                                                tACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg                                                                                                                                 >  2:217253/1‑251 (MQ=255)                                                                                                                                                              tcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAaccacc                                                                                   >  1:105873/1‑251 (MQ=255)                                                                                                                                                                accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCtg                                                                                 >  2:83674/1‑251 (MQ=255)                                                                                                                                                                      aCCACAGGTACGGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCCCCGGCCAGTGCGGGCTTTTTTTT‑CGACCAAAGTTAACGAGGTAACAACCATGCGAGTGTGGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCg                                                                           <  2:446003/251‑1 (MQ=255)                                                                                                                                                                      aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCACCCACCtggtgg                                                                             >  1:125604/1‑249 (MQ=255)                                                                                                                                                                            gatAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACAATGCGAGTGTTGAAGTTCGGCGGTTCATCAGTGGCAAATGCAGAACTTTTTCTGCGGGTTGCCGATATTCTGTAAAGCAATTCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTTCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGAtt                                                                     <  1:21230/249‑1 (MQ=255)                                                                                                                                                                                aCGGTGCGGGGTGACGCGCAAGGGAAACACAAAAAAAAGCCCGCACCTGACAGTGCGGTCTTTTTTTT‑CGACCAAAGTTAACGAGGTAACAACCATGCGAGTGTTGAAGTTGGGCGGTACATCAGTGGCAAATGCAGACCGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCTTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGaaa                                                                 <  2:492670/251‑1 (MQ=255)                                                                                                                                                                                            gaggcgTACAGGAAACACAGAAAAAAGCCCGTACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTATCGAGTTAACAACCTTGCGAGTGTTGAAGTTCGGCGGTACATCTTTGTCAAATGCAGAACTTTTTCTTCGGGTTTCCGATATTCTGTAAAGCAATGCCAGGAAGGGGCAGGTGGCCACCTTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGc                                                      <  1:468293/247‑1 (MQ=255)                                                                                                                                                                                                         aaaacaaaaaaaaGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACAAAAGGTAACGAGGTAACAACCATGCGAGTGTGGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCGGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGGGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCttt                                        <  2:305598/244‑1 (MQ=255)                                                                                                                                                                                                          accaaGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATCCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTa                                       <  1:42340/247‑1 (MQ=255)                                                                                                                                                                                                                        gcGCACCTGAAAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAAAAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGAAATGCCAGGCAGGGGCAGGGGGGCAGCGTAATCTCTGCACCCGCCAAAATCAACAAACACATGGTGGCGATGGTTGAAAAAAACATTAGCGGCAAGGGTGCTTTAAACAatatggat                           >  2:221102/2‑245 (MQ=255)                                                                                                                                                                                                                        ccGCACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAatat                               <  1:221102/245‑1 (MQ=255)                                                                                                                                                                                                                           ccccTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATGAGCGTCCAGGATGCTTTACCCAATAGCAGCGATGc                      <  1:391447/249‑1 (MQ=255)                                                                                                                                                                                                                           cACCTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCCATAACAGCGGTGc                      >  2:215490/1‑251 (MQ=255)                                                                                                                                                                                                                             ccTGACAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCg                    <  1:83674/251‑1 (MQ=255)                                                                                                                                                                                                                                               ttttttCGACCAAAGGAAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGCTATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAACTCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAAGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGa    >  1:449023/1‑250 (MQ=255)                                                                                                                                                                                                                                                tttttCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGCTGCTTGAAAAAACCATTAGCGGCCCGGAATCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGACGAAc  >  2:68595/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ttttCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAAc  <  1:217253/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       CAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAAC  >  NC_000913/67‑556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑