Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,627,299 2 bp→GA intergenic (+5/‑15) radA → / → nadR DNA recombination protein/DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,627,2990AG94.7% 110.0 / ‑5.9 38intergenic (+5/‑16)radA/nadRDNA recombination protein/DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (13/23);  total (14/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,627,3000TA88.9% 97.2 / ‑2.4 38intergenic (+6/‑15)radA/nadRDNA recombination protein/DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base A (9/23);  total (14/24)
Fisher's exact test for biased strand distribution p-value = 9.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑ATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTT  >  NC_000913/4627057‑4627532
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                        
ttCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTTTAATGAG‑GTATACgg                                                                                                                                                                                                                                    >  1:373951/1‑250 (MQ=255)
   cAGCCTGCGTGACAGACCGCGGCCACAGGATCTGGTGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACggaggg                                                                                                                                                                                                                                <  2:77316/251‑1 (MQ=255)
         gcgtgACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGGGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGGAGGGATGCAGGTTTTTGGTGTTAAAGAACTCTCCGTCGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GCATCCGGAGGGAGatat                                                                                                                                                                                                                          >  1:177934/5‑251 (MQ=255)
          cgcgACAGCCCGCGGCCGCAGGATCTGGTGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCACTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGTTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGATTAGCGTGTTCGACGACTTATAAGGAG‑ATATACGGAGGGAGATATg                                                                                                                                                                                                                         <  2:251894/251‑1 (MQ=255)
              aCAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATgtcgt                                                                                                                                                                                                                     <  1:65750/251‑1 (MQ=255)
                tgACCGCTGCCACAGGATCTGGTGGAGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGGAACCCGGTTTTCGCCGGGCGCTATTTCCGGCGGCTAAAGGACCGAAAACAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTTTCCGACGCGCTTAACGTGTTAGACGCCTTATAATGAGGAT‑TACGGGGGGAGATATgtcgtct                                                                                                                                                                                                                   >  2:341315/2‑250 (MQ=255)
                  aCCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCAtt                                                                                                                                                                                                                 <  1:201289/251‑1 (MQ=255)
                      cTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGGAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGCGATATGTCGTCATTTGAt                                                                                                                                                                                                             >  2:326865/1‑251 (MQ=255)
                              ggATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGTGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa                                                                                                                                                                                                     <  1:68002/251‑1 (MQ=255)
                                       tgtgtTTGGGGAAGTCGGGCTGGCAGGGGAGATCCGCGCGGTGCCCAGCGGTCATGAACGAAGTGCAGAAGCGGCGAAACACGGTTTTCGGCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCTGAATGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAAGACGGAGGGAGATATGTCGTCATTTGATTTCCTGAAAACTGCCa                                                                                                                                                                                             <  2:24155/249‑1 (MQ=255)
                                       ggtgTTAGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCCGCGGTAAGGGACGAATTTCAGAAGAGGCGAAACACGGGTTTCGCCGGGCGATTGATCCGGCGGCTAAAGCACCGAAAAAAGCGCCGAAAGGGATGAAAATTTTTGGCGTTAAAAACCTCTCCAAAGAGATTAGCGTGTTCGCAGACTTATAATGAG‑GTACACGGGGGGGGAGATGTAGTCATTTGTTTACCTGAAAAATGCCAt                                                                                                                                                                                            >  2:246524/1‑251 (MQ=255)
                                                   aGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAggg                                                                                                                                                                                <  1:326865/251‑1 (MQ=255)
                                                      cGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCt                                                                                                                                                                              <  1:15844/250‑1 (MQ=255)
                                                        ggCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCa                                                                                                                                                                           <  1:341315/251‑1 (MQ=255)
                                                                ggggAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTAcag                                                                                                                                                                   <  1:106596/251‑1 (MQ=255)
                                                                      aTCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGtg                                                                                                                                                             <  1:334276/251‑2 (MQ=255)
                                                                            ccGGTGCCCAGCGTTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTAGGCGTTAAAAAAATCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATAAGGAGTGAGATAGGTCGTCATTTGATTACCTGAACACTGCCATCAAGCAAATGGACTGCAAGCTACAGCAGGTGGCTGAt                                                                                                                                                       >  2:207595/1‑251 (MQ=255)
                                                                                ggCCCGGCGGTCAGGAACGAATTTCAGCAGCGGCGAAAAACGGTGTTCGCCGGGGGATGGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTGTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAAGACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCa                                                                                                                                                   <  2:188408/250‑1 (MQ=255)
                                                                                  cccAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGc                                                                                                                                                 >  1:350511/1‑251 (MQ=255)
                                                                                     aGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGTGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGt                                                                                                                                              <  1:371700/251‑1 (MQ=255)
                                                                                                    atttcagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGTGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTAt                                                                                                                               <  1:207164/245‑1 (MQ=255)
                                                                                                             gcggcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg                                                                                                                      <  1:242210/251‑1 (MQ=255)
                                                                                                                  gAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACt                                                                                                                 <  1:246524/251‑1 (MQ=255)
                                                                                                                   aaaCACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTg                                                                                                                >  1:252518/1‑251 (MQ=255)
                                                                                                                                                   ggcTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACGCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGTGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaaaa                                                                                <  1:193848/251‑1 (MQ=255)
                                                                                                                                                                   aaaGCGCCGGAAGAGATGCAGATTTTTGACGTTAAAAAACTCTACGATGAGCTTAGCGTGTTCGAAGACTTATAATGAG‑GGATACGGAGGGAGATATGTTGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCAGAACGCTACAGCAGGAGGCTGATGCCAGCGGGATGACCAAAGGGTATTAAAGCCAGTGACTGAATGCAAAAATACAATGCCCCAGGGCGCAAAAGCTGGAGGCGTTgaac                                                                >  2:193118/1‑248 (MQ=255)
                                                                                                                                                                       cgcCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGGGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTTGCTGATGCCAGCGGTATGACCACAGGGTGTTGACGCAAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGAGCAAAAGCTGGAGGCGTTGCACCGtg                                                            >  2:149187/1‑250 (MQ=255)
                                                                                                                                                                           ggAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGtttttt                                                        >  1:100762/1‑251 (MQ=255)
                                                                                                                                                                            gAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTg                                                       <  1:17380/251‑1 (MQ=255)
                                                                                                                                                                               gggATGCAGATTTTTGGCGTTAAAAAACTCGCCGACGCGCTAAGAGTGTTCGACGACTTATAATGGG‑GAATACGGAGGGAGATATTTCGTCATTTGATTACCTGAAAACTGCCATCACGCAACAGGGATGCACGCTAAAGCAGGTGGCTGATGCCAGCGGTATGCCCAAAGGGTAGTTACGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGATGCACCGGTTTTTgtgg                                                    >  2:311302/1‑248 (MQ=255)
                                                                                                                                                                                tgATGAAGATTTTTGGCGTTAAACAACTCTCAGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGATGGAGATATGTCGTCATTTGATTACCTGAAAGCTGCCATCAAGAAACAGGGCTGCATGCTACAGCAGTTGGCTGATGCCAGCGGTATGCCCACAGGGTATTTAAGCCAGTTACTGTATGCCTAAATCAAAAGCCCCATCGCGAACAAGCTGGAGGCGTGGCACCGTTTTTAGGGGc                                                   >  2:314910/2‑251 (MQ=255)
                                                                                                                                                                                       gATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGTGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACTGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTGTGGGGCTTGAGtt                                            <  1:67945/251‑1 (MQ=255)
                                                                                                                                                                                       gATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGtt                                            <  1:116860/251‑1 (MQ=255)
                                                                                                                                                                                              ggCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTAt                                                                                                                                            >  2:351541/1‑148 (MQ=255)
                                                                                                                                                                                              ggCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTAt                                                                                                                                            <  1:351541/148‑1 (MQ=255)
                                                                                                                                                                                                                                 cgacgtCTTATAATGAG‑GAATAAGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCATCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGt   <  2:149417/250‑1 (MQ=255)
                                                                                                                                                                                                                                 cgacgaCTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGtt  <  1:51748/251‑1 (MQ=255)
                                                                                                                                                                                                                                 cgacgaCTTATAATGAG‑GAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGtt  <  1:380341/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                        
TTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAG‑ATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTT  >  NC_000913/4627057‑4627532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑