Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,331,059 2 bp→AT coding (12‑13/2598 nt) topA → DNA topoisomerase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,331,0590TA95.8% 61.8 / NA 25A4A (GCT→GCAtopADNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (16/7);  minor base C (1/0);  total (17/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
*NC_0009131,331,0600CT100.0% 71.2 / NA 25L5F (CTT→TTT) topADNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/8);  total (17/8)

CGCTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTAT  >  NC_000913/1330817‑1331296
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                            
cGCTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCAt                                                                                                                                                                                                                                       >  1:315495/1‑251 (MQ=255)
  cTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCg                                                                                                                                                                                                                                     >  1:287247/1‑251 (MQ=255)
               ggCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTcccc                                                                                                                                                                                                                           <  1:371451/248‑1 (MQ=255)
                           gCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCaaaa                                                                                                                                                                                                             <  1:201008/250‑1 (MQ=255)
                              tGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGAt                                                                                                                                                                                                         >  1:303704/1‑251 (MQ=255)
                                             aTTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTggg                                                                                                                                                                                          >  1:82256/1‑251 (MQ=255)
                                              ttCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGt                                                                                                                                                                                         >  1:19248/1‑251 (MQ=255)
                                                      tGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACt                                                                                                                                                                                  >  1:91589/1‑250 (MQ=255)
                                                                     aGGCCAAGACATGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTAACGACAAGAGGTTGATATCCGAAGTGAGCGAGTCCATATCGGACACTCGTTGCCAGAGGAAGGATTATCAACATGCGACGCATTCCTGGAAAAATCAAATTAGGTCAGTTGAATATGGGTAAAGCATTTGTAATCGTTGAGTCCCAGGCATAAGCCAAAACGATCAACAAGTAAATGGGGAGTGACTACGTGGTGAAAtaca                                                                                                                                                                   >  2:374937/1‑247 (MQ=255)
                                                                                                  caTTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGCGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCACATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCAATTTGCCGAcc                                                                                                                                      >  2:286509/2‑250 (MQ=255)
                                                                                                          tGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTc                                                                                                                                                              >  1:26972/1‑218 (MQ=255)
                                                                                                          tGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACACGTTGACAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTTGCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGGATCTGGGTAGTGATTACGTGGTTAAATCCAGCGTc                                                                                                                                                              <  2:26972/218‑1 (MQ=255)
                                                                                                                                   atatCCGCAGAGAGCGAGTCCATATCGGTATCTCTTTGACAGTGTAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTATGTAAGGTGAATATGTGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGtctcg                                                                                                     <  2:33495/250‑2 (MQ=255)
                                                                                                                                     atCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGCGTGCCGACTCTaac                                                                                                  >  2:247209/1‑249 (MQ=255)
                                                                                                                                                            cGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACACGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCCAAAAGAGTGCCGACTCTACCTCAACCAAGACGGCTaaaaatcc                                                                           >  2:60194/1‑248 (MQ=255)
                                                                                                                                                             ggTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCt                                                                          >  1:82518/1‑251 (MQ=255)
                                                                                                                                                                     gTTGCCAGTGGAAGGTATATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGTTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGa                                                                  <  1:46458/251‑1 (MQ=255)
                                                                                                                                                                          taGTGGAAGGTTTATCCACGTGCGACGCATTACTGGAAGAATCACATTAGGTAAGGTGAATATGGGTAACGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGAAGTGACTACGTGGTGAACTCCAGCGTCGGCCACATCCGCGCTTTGCCGACCATAGGCTCAGCTGCCACACAGAGTGCCGACGCTACCTCCACCCAGCCGGCTAAAAAGCCTAAATAGGCTGAAc                                                             >  2:27793/2‑251 (MQ=255)
                                                                                                                                                                            ggggAAGGTTTATTAACGGGCGACGCATTCCTGGAAGAATCAAATAAGGTAAAGTGAATATGGGTAAAGCATTAGGCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAGCAAGTATCTGGGTAGAGACGAGGTGGTGAAATCCAGCTTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCGGCCAAAAAGGGTGCCGACACTACCGCCACCAAGACGGCTGAAAATCCTAAAATGTATGAACGt                                                           <  2:346333/249‑1 (MQ=255)
                                                                                                                                                                              ggAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTg                                                          <  1:60194/250‑1 (MQ=255)
                                                                                                                                                                                        tCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCCTTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGACAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTc                                               >  1:41759/1‑251 (MQ=255)
                                                                                                                                                                                                                       aTTAGGTAAGGAGAATAGGGATAAAGCATTTGTCATCGATGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGCAAAATCCGCGATTTGCCGACCAGTGGCTCATCTGCCAAAGAGAGTGCAGACTCTACCTCCACCAAGACGCCTAAAAAGACTAACAAGGATGAACGGGGCGCTCCAGGCAACCTTATGGGGGTTTACCCGAGGCAAAAtt                >  2:294058/1‑251 (MQ=255)
                                                                                                                                                                                                                           ggTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTggg             >  1:73114/1‑250 (MQ=255)
                                                                                                                                                                                                                              aaGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgc         >  1:91757/1‑251 (MQ=255)
                                                                                                                                                                                                                                     ataggggTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAATCGATCAACAAGTAGCTGGGTAGTGACTACGGGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTAt  <  2:91589/247‑1 (MQ=255)
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                            
CGCTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTAT  >  NC_000913/1330817‑1331296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: