Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 8,975 2 bp→TA coding (738‑739/954 nt) talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009138,9750GT95.7% 62.3 / ‑3.0 23P246P (CCG→CCT) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (10/12);  total (10/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01
*NC_0009138,9760GA95.7% 60.0 / ‑4.1 23A247T (GCA→ACA) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (10/12);  total (10/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

CCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTC  >  NC_000913/8756‑9216
                                                                                                                                                                                                                             ||                                                                                                                                                                                                                                                
ccTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGCGCTGGCTGAGAGCGa                                                                                                                                                                                                                      >  1:323583/1‑249 (MQ=255)
           ccatggttgGCCGTATTCTTTACTGGTACAAAGCGAATACCGATAAGAA‑AGAATAAGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATATGAAATCTACCAGTACTACAAAAAGCCCGGTTATGAACCCGTGGTTATGGGCGAAAGCTTCCTTAACATCGGCGAAATTCTGGAAATGGCATACTGAGTACTG‑CTGACCATAGCACATACACTGCGGAAAGAGATGGCAGAGGGCGAAGGGACTATCGa                                                                                                                                                                                                          >  2:205765/6‑250 (MQ=255)
            ggttgttgGCCGTATGCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGagag                                                                                                                                                                                                                         <  2:273625/232‑1 (MQ=255)
            gTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGagag                                                                                                                                                                                                                         >  1:273625/1‑234 (MQ=255)
                         aTTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTa                                                                                                                                                                                           <  1:259226/251‑1 (MQ=255)
                         aTTCGTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTa                                                                                                                                                                                           <  1:236379/251‑1 (MQ=255)
                             gagggttggaaaaaGCGATTAGCGATA‑GAACAGAGTGCGCGCGGGCAGAAGAGGCGGGCGTGGTTTCTGTAGCTGAAACGTACTAGTACACCAAAGAGCGGGGGTATGAAACGGGGGTTTTGGGGGCAAGCTGCGGTAAAAGCGGCGAAATTCGGGAACTGGCAGGCTGCG‑GCCGTCTGTGCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACAcc                                                                                                                                                                                       <  2:259904/240‑1 (MQ=255)
                              tGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACAcc                                                                                                                                                                                       <  1:267408/250‑1 (MQ=255)
                                                     aTAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGgcg                                                                                                                                                               >  1:328906/1‑251 (MQ=255)
                                                               agTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACt                                                                                                                                                      <  1:65202/251‑1 (MQ=255)
                                                                             gaagaTCCGGGCGTGGTTTCTGTATCTGAAATCTAACAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCt                                                                                                                                        >  1:215528/1‑251 (MQ=255)
                                                                                gaTCCGGGCGAGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAAAATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCtgtg                                                                                                                                     >  1:98382/1‑251 (MQ=255)
                                                                                               tctttATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGACATGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAgg                                                                                                                       <  2:66311/246‑1 (MQ=255)
                                                                                                              tcccAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGGGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTATTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGCTCCAATGGCAGTAg                                                                                                        <  1:139893/248‑1 (MQ=255)
                                                                                                                      ctacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTgg                                                                                               >  1:59470/1‑251 (MQ=255)
                                                                                                                                                 ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTg                                                                    <  1:107483/251‑1 (MQ=255)
                                                                                                                                                        ggCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAgg                                                              >  1:102706/1‑250 (MQ=255)
                                                                                                                                                                 ttCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGCCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTgg                                                     >  1:164022/1‑250 (MQ=255)
                                                                                                                                                                     ggaatatatgagAAATTTTGGAACTGGCGGGTTGCG‑ACAGTATTGCCAGCGCACCTACACGGCTGAAAGAGGTGGCTGAGAGCGAAGGGGCTATCTAACGTAACCTGTCTTACACCGGCGAAGTGAAAGCTCGTCCGGCTGGTATCGCTGAGTCCGAGTTCCGGTGGCAGCCCAACCATGATCCAATGGCAGTAGAAAATGTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACGGGaaaaa                                                <  2:149523/240‑1 (MQ=255)
                                                                                                                                                                                                 aGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGCGTTCCTGTGGAAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTGTTGACCAAGAAAAACGGGAAAAAAAGAACGGCGATTCGCTGTAATCATTCt                    >  2:96581/1‑251 (MQ=255)
                                                                                                                                                                                                  ggCTGCG‑ACAGTCTTACCATCACACCTACACTGCTGAAAGAGCTGGCTGAGGGCGAAGGGGCTATCGAACGTAAACTGTCTTACTCCGGCGAAGTTAAAGCGCGTCCGGCGCTTATCAATGGGTCCGAGTGTCTGTTGCAGCTCAATCAGTATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCtt                   <  2:121953/251‑1 (MQ=255)
                                                                                                                                                                                                           ccGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGGCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGgc                                                                                                                                   <  1:218594/131‑1 (MQ=255)
                                                                                                                                                                                                                    cATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTTTCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTc  <  1:269769/251‑1 (MQ=255)
                                                                                                                                                                                                                             ||                                                                                                                                                                                                                                                
CCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTC  >  NC_000913/8756‑9216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑