Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,681 A→G F93F (TTT→TTC)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,6810AG94.7% 44.9 / ‑4.0 19F93F (TTT→TTC) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (6/12);  total (6/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

AAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGA  >  NC_000913/7440‑7898
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                          
aaGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCt                                                                                                                                                                                                                   >  1:75509/1‑251 (MQ=255)
               ctcgcgCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGACACTGCACATTGGCCTCACGTTCTTTATACAGCTGTGCGAGGGAACATTCGGCCAACAAGGTCGCCATGCCGATAAAAGAGGCAACACACATACAGAAGACGGCTCCAGGTACACcggcg                                                                                                                                                                                                    >  2:37610/6‑251 (MQ=255)
                         ggCGTTCGCCTGAATTCCGCTGAAAATTATGCCGAAGGAGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGACCGCGCGCCATATACCATGACGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGACGCTAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcgggaaatgccc                                                                                                                                                                                          >  1:57232/1‑242 (MQ=255)
                                                        cctagggCGATGAGCAAAAAGACGGAGAACAGAACCCCCACCCAGCCCACCCCCACCCCGCGCACCATACACCTGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCAAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATGGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                                                                                           <  2:47460/246‑1 (MQ=255)
                                                        ccgtAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATGCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                                                                                           <  1:226794/248‑1 (MQ=255)
                                                            aGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAcgcg                                                                                                                                                        <  1:228219/250‑1 (MQ=255)
                                                                gATGAGCAAAAAGTCGGTGAACAGAGCCCTCACCCCGCGCATCCCCAGCCCGCGCGCCATATCCCATGCCGGTCCTCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGTTTTGCGAGGGAAAATTCGGCAAACGAGATCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGcgccg                                                                                                                                                   <  1:227788/251‑1 (MQ=255)
                                                                                             ccATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGCCCCCACCCACCCGCGCCGCACAG‑CCGGGCCACAATGACTGAAAtagg                                                                                                                     >  1:334545/1‑248 (MQ=255)
                                                                                                   aGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTTACCGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCC‑AGACTGGGACACAATTACTGAAACGAGGttca                                                                                                                >  1:358515/1‑248 (MQ=255)
                                                                                                    gcgcATCCCCAGCCCGCGCGCCATAGACGATGCCGGGCCGCCACGAAACTGCCCATTGGCATCACGTTCATTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAAc                                                                                                              <  1:260138/251‑1 (MQ=255)
                                                                                                         cccccAGCCCGCGCGCCATATACCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCt                                                                                                         <  1:132373/250‑1 (MQ=255)
                                                                                                                                             cACGAAACTGCCCATTGGCATAACGTTCTTTATAAAGCGGGGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCa                                                                     <  1:263307/251‑1 (MQ=255)
                                                                                                                                                                          ttATAAAGCTGTGCGAGGAAACATTCGGCAACCGAGTGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGcaca                                          <  1:181972/249‑1 (MQ=255)
                                                                                                                                                                                       cgagggaACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTTCCAGCGCAACACCGGCCAGGGTGCCGCTCACCACGCGCGCCGCA‑AGACTTGTACAAAATGACTGAAATGAGGTTAAAACGCCCGGCTGTGGGTGAATGCTATTTTTAAAACTTTTGCCAAACTGGCGGATGTAGCGGAAAGACACAAATCCGGGGCGaaa                            >  1:123090/6‑250 (MQ=255)
                                                                                                                                                                                                agtCGGCAAACGAGGCCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGaa                             <  1:16061/238‑1 (MQ=255)
                                                                                                                                                                                                         ataccttacgccgggcGATAAACGCGGCATCCCGCTTCCGGAAGACGGCCCCAGGGCCGCAGGCGGTAATGGCCAGGTCTAGCCCGGCCAGGTTGCCGCTACCCACGCCGGCCGAA‑AGACGGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTgcg         <  2:224478/236‑1 (MQ=255)
                                                                                                                                                                                                           gAGGTCGCCCTGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGcc       <  1:353646/251‑1 (MQ=255)
                                                                                                                                                                                                               tCGCCAGGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCGCCGTCGGTAATATCCGGCGCAACGCCGGCCAGGTTGCGGCTACCCACGCGTGCCGCA‑AGACTGGAGCACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCCATTTTTAAGGCGTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGaa    <  1:364134/250‑1 (MQ=255)
                                                                                                                                                                                                                cGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAaga  <  1:197303/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                          
AAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGA  >  NC_000913/7440‑7898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑