Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,598	A→G	V121A (GTC→GCC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,598	0	A	G	100.0%	53.8 / NA	20	V121A (GTC→GCC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/9);  total (11/9)

CGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCA  >  NC_000913/7376‑7831                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           cgAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcg                                                                                                                                                                                                                  >  1:166877/1‑248 (MQ=255)                        gACAGCGAGAATAATACCTGCCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTCCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCACCCAGCGCGCCATATACCATGCACGTCCGCCACTAAACTGCCCCTTGGCATCACGTTCTTTATAAAGCGGTGCGAGGGAACATTCCGCCAACGAGGTCg                                                                                                                                                                                         >  2:118392/1‑251 (MQ=255)                           aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGcc                                                                                                                                                                                       >  1:124036/1‑250 (MQ=255)                                   taatACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAAAGCCCATCCAGCGCATCCCCAGCCCGCGCGCCCTATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAAAGAGGTCGCATTGCCGAt                                                                                                                                                                               >  2:374844/1‑250 (MQ=255)                                               ccAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAAcc                                                                                                                                                                  <  1:374844/251‑1 (MQ=255)                                                            aTCAAAAGAAAAACTCAGGGCTCACGAAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAACGACGGCGAACCAAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGAATCACGTTCTTTATAAAGATGTGCGATGGAACATTCGGCAAACATGGTCGCCATGCCGATAAAAGCGGCAACCCCCATCCaga                                                                                                                                                        >  2:61297/1‑246 (MQ=255)                                                                 aaGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCt                                                                                                                                                >  1:75509/1‑251 (MQ=255)                                                                                ctcgcgCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGACACTGCACATTGGCCTCACGTTCTTTATACAGCTGTGCGAGGGAACATTCGGCCAACAAGGTCGCCATGCCGATAAAAGAGGCAACACACATACAGAAGACGGCTCCAGGTACACcggcg                                                                                                                                 >  2:37610/6‑251 (MQ=255)                                                                                          ggCGTTCGCCTGAATTCCGCTGAAAATTATGCCGAAGGAGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGACCGCGCGCCATATACCATGACGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGACGCTAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcgggaaatgccc                                                                                                                       >  1:57232/1‑242 (MQ=255)                                                                                                                         cctagggCGATGAGCAAAAAGACGGAGAACAGAACCCCCACCCAGCCCACCCCCACCCCGCGCACCATACACCTGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCAAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATGGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                        <  2:47460/246‑1 (MQ=255)                                                                                                                         ccgtAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATGCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                        <  1:226794/248‑1 (MQ=255)                                                                                                                             aGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAcgcg                                                                                     <  1:228219/250‑1 (MQ=255)                                                                                                                                 gATGAGCAAAAAGTCGGTGAACAGAGCCCTCACCCCGCGCATCCCCAGCCCGCGCGCCATATCCCATGCCGGTCCTCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGTTTTGCGAGGGAAAATTCGGCAAACGAGATCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGcgccg                                                                                <  1:227788/251‑1 (MQ=255)                                                                                                                                                              ccATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGCCCCCACCCACCCGCGCCGCACAG‑CCGGGCCACAATGACTGAAAtagg                                                  >  1:334545/1‑248 (MQ=255)                                                                                                                                                                    aGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCa                                                                                                                                                                                      <  1:169348/114‑1 (MQ=255)                                                                                                                                                                    aGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCa                                                                                                                                                                                      >  2:169348/1‑114 (MQ=255)                                                                                                                                                                    aGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTTACCGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCC‑AGACTGGGACACAATTACTGAAACGAGGttca                                             >  1:358515/1‑248 (MQ=255)                                                                                                                                                                     gcgcATCCCCAGCCCGCGCGCCATAGACGATGCCGGGCCGCCACGAAACTGCCCATTGGCATCACGTTCATTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAAc                                           <  1:260138/251‑1 (MQ=255)                                                                                                                                                                          cccccAGCCCGCGCGCCATATACCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCt                                      <  1:132373/250‑1 (MQ=255)                                                                                                                                                                                                              cACGAAACTGCCCATTGGCATAACGTTCTTTATAAAGCGGGGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCa  <  1:263307/251‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           CGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCA  >  NC_000913/7376‑7831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑