Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,483	C→T	A181V (GCC→GTC)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,483	0	C	T	95.0%	51.1 / ‑3.6	20	A181V (GCC→GTC)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (8/11);  total (8/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-01

AAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTG‑ATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4641240‑4641652                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            aaaaaTCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATggc                                                                                                                                                                                                                                       >  1:383048/1‑248 (MQ=255)             ggATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGAct                                                                                                                                                                                                                          <  1:90097/251‑1 (MQ=255)                                                      gggctGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAAACGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATCAAACTGGTCGACTGGTTac                                                                                                                                                                                  >  1:370129/5‑250 (MQ=255)                                                                             gTTGGCTGACGTTCTTACTGGTGTGCCGAGGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCATCTGCAAGCTTTACGCGAACGAGTCATGGCATTGCGGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGc                                                                                                                                                          <  1:244754/251‑1 (MQ=255)                                                                                       gttatgaCTGGTGCTCCGATGGTGGCAGATTATCCGTCGCTCAATCGGGGGCAGGCAGTGATTGACTATTGCTACCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCAGTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACGGGTCGACTGGTTACAGCAACGCCTGGGTCTTTTAGATCAACGAGacac                                                                                                                                                <  2:19368/245‑1 (MQ=255)                                                                                                              ggcagATTATCCGTCGCGCAATCTGGGGCAGGCAGGGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACCCAACGGCAGACCAACATCAACTGCAAGCTGTACGCGAACGAGTCATGGCATTGCTGACGGCTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTtgctg                                                                                                                          <  2:363576/246‑1 (MQ=255)                                                                                                                    ttATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTG‑ATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTagag                                                                                                                                                           <  1:97775/211‑1 (MQ=255)                                                                                                                                                     ggTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCtt                                                                                  >  1:275031/1‑251 (MQ=255)                                                                                                                                                            tGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGacac                                                                                                                                                <  1:297894/182‑1 (MQ=255)                                                                                                                                                            tGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGacac                                                                                                                                                >  2:297894/1‑182 (MQ=255)                                                                                                                                                             gCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATTGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTagtaagt                                                                           <  1:343633/250‑1 (MQ=255)                                                                                                                                                               tATCAATTAGCAACATTAATACAACAACCGCCGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTtgct                                                                                                                           >  1:258650/1‑200 (MQ=255)                                                                                                                                                               tATCAATGAGCAACAGTAAGACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTGTACGCGAACGAGTCATGTCATTGCGGACGACTCTGGCAGTGTCAGATGACATAAAACGGGTCGTCTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCTTTtgct                                                                                                                           <  2:258650/200‑1 (MQ=255)                                                                                                                                                                 acaaatAGCAACATTAATACAACAACCGACGAAAA‑TGTACACAACGGCAGACCAACAGCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTAtttt                                                                      <  2:275031/246‑1 (MQ=255)                                                                                                                                                                         cAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTTCGCGAACGAGTCATGGCATTGCTGACGACGCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACTAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttt                                                              >  1:15895‑M2/1‑245 (MQ=255)                                                                                                                                                                                              cGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctaactgaaacgggc                                          <  2:351288‑M2/250‑27 (MQ=255)                                                                                                                                                                                                      tgacacaacggCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTCGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagattttcattctgactgcaacgggcaatatgtc                                  <  1:35024‑M2/246‑35 (MQ=255)                                                                                                                                                                                                            aaCGGCAGACCAACATCAACTGCAAGCTGTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGaaaa                                                                                                            <  1:36126/171‑1 (MQ=255)                                                                                                                                                                                                                          aTCAACTTCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTTGCAGATGACATAAAACTGGTCTACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaag              >  1:370817‑M2/1‑197 (MQ=255)                                                                                                                                                                                                                                        ttACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatag  >  1:351288‑M2/1‑183 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            AAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTG‑ATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4641240‑4641652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑