Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,428	G→A	A163T (GCG→ACG)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,428	0	G	A	88.9%	41.6 / NA	19	A163T (GCG→ACG)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (6/10);  minor base C (2/0);  total (8/11)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTG‑ATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑  >  NC_000913/4641188‑4641652                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        gCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGCCGAAAAGTg                                                                                                                                                                                                                                >  2:90097/1‑250 (MQ=255)                                    ggtaccGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATGCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTAc                                                                                                                                                                                           <  1:47813/247‑1 (MQ=255)                                    ggtaccGCTGTTAGAGGAAAAATCTGCATGGATGAGCCAGGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGAATAACGAATAGTGAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTAc                                                                                                                                                                                           <  2:313326/247‑1 (MQ=255)                                          gCTGTTAGAGGAAAAATCTTCATGGATGAGACATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTAcgcgcac                                                                                                                                                                                     >  1:297418/1‑248 (MQ=255)                                                     aaaaaTCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATggc                                                                                                                                                                           >  1:383048/1‑248 (MQ=255)                                                                 ggATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGAct                                                                                                                                                              <  1:90097/251‑1 (MQ=255)                                                                                                          gggctGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAAACGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATCAAACTGGTCGACTGGTTac                                                                                                                      >  1:370129/5‑250 (MQ=255)                                                                                                                                 gTTGGCTGACGTTCTTACTGGTGTGCCGAGGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCATCTGCAAGCTTTACGCGAACGAGTCATGGCATTGCGGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGc                                                                                              <  1:244754/251‑1 (MQ=255)                                                                                                                                           gttatgaCTGGTGCTCCGATGGTGGCAGATTATCCGTCGCTCAATCGGGGGCAGGCAGTGATTGACTATTGCTACCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCAGTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACGGGTCGACTGGTTACAGCAACGCCTGGGTCTTTTAGATCAACGAGacac                                                                                    <  2:19368/245‑1 (MQ=255)                                                                                                                                                                  ggcagATTATCCGTCGCGCAATCTGGGGCAGGCAGGGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACCCAACGGCAGACCAACATCAACTGCAAGCTGTACGCGAACGAGTCATGGCATTGCTGACGGCTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTtgctg                                                              <  2:363576/246‑1 (MQ=255)                                                                                                                                                                        ttATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTG‑ATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTagag                                                                                               <  1:97775/211‑1 (MQ=255)                                                                                                                                                                                                         ggTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCtt                      >  1:275031/1‑251 (MQ=255)                                                                                                                                                                                                                tGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGacac                                                                                    <  1:297894/182‑1 (MQ=255)                                                                                                                                                                                                                tGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGacac                                                                                    >  2:297894/1‑182 (MQ=255)                                                                                                                                                                                                                 gCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATTGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTagtaagt               <  1:343633/250‑1 (MQ=255)                                                                                                                                                                                                                   tATCAATTAGCAACATTAATACAACAACCGCCGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTtgct                                                               >  1:258650/1‑200 (MQ=255)                                                                                                                                                                                                                   tATCAATGAGCAACAGTAAGACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTGTACGCGAACGAGTCATGTCATTGCGGACGACTCTGGCAGTGTCAGATGACATAAAACGGGTCGTCTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCTTTtgct                                                               <  2:258650/200‑1 (MQ=255)                                                                                                                                                                                                                     acaaatAGCAACATTAATACAACAACCGACGAAAA‑TGTACACAACGGCAGACCAACAGCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTAtttt          <  2:275031/246‑1 (MQ=255)                                                                                                                                                                                                                             cAACATTAATACAACAACCGACGAAAAGTG‑ACACAACGGCAGACCAACATCAACTGCAAGCTTTTCGCGAACGAGTCATGGCATTGCTGACGACGCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACTAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttt  >  1:15895‑M2/1‑245 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        GCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTG‑ATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑  >  NC_000913/4641188‑4641652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑