Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,296	T→C	L119L (TTG→CTG)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,296	0	T	C	100.0%	66.5 / NA	23	L119L (TTG→CTG)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/10);  total (13/10)

CAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACT  >  NC_000913/4641050‑4641502                                                                                                                                                                                                                                                        |                                                                                                                                                                                                               cAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGAc                                                                                                                                                                                                            >  1:66593/1‑251 (MQ=255)  aGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCCGTCGGGCGAAATATCATTACTACGCTACGCCCGTAGAACTGGTACCGCTGTTAGCGGGAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACt                                                                                                                                                                                                           >  2:47813/1‑251 (MQ=255)                cctgccaCCCTCTGGGTCGCACAGGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGTTCGAAATATCATTTCTACGCTACGCCAGTAGAACTGGTACCGCGGTTAGAGGAAAAATCTTCATGGAGGGGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGc                                                                                                                                                                                             <  1:236985/248‑1 (MQ=255)                      aCCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCACGACACTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGACGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGTAGAGTTAGCGTTGGc                                                                                                                                                                                       >  1:306682/1‑251 (MQ=255)                             gggTCGCCCATGGATCGGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCTCTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGACGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGt                                                                                                                                                                                 <  1:46100/250‑1 (MQ=255)                                     caTGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTgg                                                                                                                                                                        >  1:313326/1‑251 (MQ=255)                                              ggtattatatatgatatatttAAAGTTTTCCCGACACTGGCTGAATCGTTACGCGATGTCGATTTC‑ACTGTCTCCACGACGGCGCGCAGTCGGGCGAAATAGCATTACTACGCTACGCCAGTCGAACGGTTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGAGCGGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGAt                                                                                                                                                               <  2:142672/232‑1 (MQ=255)                                                    attattTAAAATATTAAAGTTGTCCCGACACGGGTTGAATCGGTACACGATGTCG‑TTTCTACTTTCGCCACCTCTGCGCGCAGTCGGGCGAAATATCATTACTACGCTTCGCCATTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGTCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGAtggtgg                                                                                                                                                          <  2:304579/250‑1 (MQ=255)                                                    attattGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCAACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGATAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGTAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGc                                                                                                                                                         >  1:84805/1‑251 (MQ=255)                                                                     aGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCa                                                                                                                                        <  1:206155/251‑1 (MQ=255)                                                                             cGACACTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTgggg                                                                                                                                >  1:207890/1‑251 (MQ=255)                                                                                        gAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGCGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGAt                                                                                                                     >  1:18769/1‑251 (MQ=255)                                                                                        gAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGAt                                                                                                                     <  1:360292/251‑1 (MQ=255)                                                                                                    gATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCt                                                                                                          >  1:75912/1‑250 (MQ=255)                                                                                                          gATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAAtt                                                                                                   <  1:323728/251‑1 (MQ=255)                                                                                                                             acTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATacaac                                                                                  >  1:363576/1‑250 (MQ=255)                                                                                                                                     cAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCgacg                                                                          >  1:159903/1‑247 (MQ=255)                                                                                                                                            gCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGCCGAAAAGTg                                                                   >  2:90097/1‑250 (MQ=255)                                                                                                                                                                               ggtaccGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATGCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTAc                               <  1:47813/247‑1 (MQ=255)                                                                                                                                                                               ggtaccGCTGTTAGAGGAAAAATCTGCATGGATGAGCCAGGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGAATAACGAATAGTGAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTAc                               <  2:313326/247‑1 (MQ=255)                                                                                                                                                                                     gCTGTTAGAGGAAAAATCTTCATGGATGAGACATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTAcgcgcac                         >  1:297418/1‑248 (MQ=255)                                                                                                                                                                                                aaaaaTCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATggc               >  1:383048/1‑248 (MQ=255)                                                                                                                                                                                                            ggATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGAct  <  1:90097/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                               CAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTC‑ACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACT  >  NC_000913/4641050‑4641502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑