| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 7,479 | C→T | V161I (GTT→ATT) | yaaJ ← | putative transporter YaaJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 7,479 | 0 | C | T | 91.3% | 56.9 / ‑3.6 | 23 | V161I (GTT→ATT) | yaaJ | putative transporter YaaJ |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (12/9); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCA > NC_000913/7232‑7715 | ccaAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAAGCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGCGCAACGGCGTTCGCCTGAATTcc < 2:137379/251‑1 (MQ=255) cAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATg > 1:47460/1‑251 (MQ=255) aaGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACgg > 1:181359/1‑251 (MQ=255) ttGCCCGATCTTCATTAAGCAAATCACCAGGCTGATCAGTACCCAGATTATCGTCATAAACGGAACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGTGCAAAGCCAGCGCGAATAAAACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGCAACTTATGCCGGAGGCGAGGAGCCAAAAGACgacg > 2:353646/1‑247 (MQ=255) atatTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATCGGCGATGAGCAAACAGACGGCGAACAGAAc > 2:197303/1‑251 (MQ=255) tCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATGATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGcc < 1:304319/250‑1 (MQ=255) aCGCAAATCACCAGGCTGGTCAGTACCCAGATTAACGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCa < 2:296591/251‑1 (MQ=255) caCCAGGCTGGGCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATcc < 1:153167/250‑1 (MQ=255) aGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAg < 1:166253/251‑1 (MQ=255) gggACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAgg > 1:177091/1‑169 (MQ=255) gggACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAgg < 2:177091/169‑1 (MQ=255) cAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAAc < 1:306144/251‑1 (MQ=255) aaGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTg < 1:47139/251‑1 (MQ=255) gCATGAGCCGGGCGACGCCATTAAGACCGCGAGTGATCGCCAGAAGAGCATAGACAGCGAGAATAATCCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCACCGGCGGTCTCCTGAATCCCGCTGAAAATTAGGCCTTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCTCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTg < 2:167865/251‑1 (MQ=255) cgAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcg > 1:166877/1‑248 (MQ=255) gACAGCGAGAATAATACCTGCCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTCCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCACCCAGCGCGCCATATACCATGCACGTCCGCCACTAAACTGCCCCTTGGCATCACGTTCTTTATAAAGCGGTGCGAGGGAACATTCCGCCAACGAGGTCg > 2:118392/1‑251 (MQ=255) aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGcc > 1:124036/1‑250 (MQ=255) taatACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAAAGCCCATCCAGCGCATCCCCAGCCCGCGCGCCCTATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAAAGAGGTCGCATTGCCGAt > 2:374844/1‑250 (MQ=255) ccAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAAcc < 1:374844/251‑1 (MQ=255) aTCAAAAGAAAAACTCAGGGCTCACGAAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAACGACGGCGAACCAAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGAATCACGTTCTTTATAAAGATGTGCGATGGAACATTCGGCAAACATGGTCGCCATGCCGATAAAAGCGGCAACCCCCATCCaga > 2:61297/1‑246 (MQ=255) aaGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCt > 1:75509/1‑251 (MQ=255) ctcgcgCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGACACTGCACATTGGCCTCACGTTCTTTATACAGCTGTGCGAGGGAACATTCGGCCAACAAGGTCGCCATGCCGATAAAAGAGGCAACACACATACAGAAGACGGCTCCAGGTACACcggcg > 2:37610/6‑251 (MQ=255) ggCGTTCGCCTGAATTCCGCTGAAAATTATGCCGAAGGAGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGACCGCGCGCCATATACCATGACGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGACGCTAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcgggaaatgccc > 1:57232/1‑242 (MQ=255) | CCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCA > NC_000913/7232‑7715 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |