Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,640,864	A→G	intergenic (+93/‑78)	ytjE → / → yjtD	protein YtjE/putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,640,864	0	A	G	84.2%	39.7 / 1.0	19	intergenic (+93/‑78)	ytjE/yjtD	protein YtjE/putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (9/7);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

TTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACA‑TGGATCTGGTGATATT  >  NC_000913/4640621‑4641103                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 tttGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGAATTACGTTAAGGATTTGt                                                                                                                                                                                                                                            >  1:265719/1‑250 (MQ=255)    gatgaAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAAt                                                                                                                                                                                                                                        >  1:105252/1‑251 (MQ=255)         aaTCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCa                                                                                                                                                                                                                                   <  1:219692/251‑1 (MQ=255)                             aGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGt                                                                                                                                                                                                               >  1:99261/1‑251 (MQ=255)                                                        cAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTGTTATAAAATTATCCAAAGTAAACAGAGGGATATGTAGCATTTTTTAACAATTCAACCGTTAATACAGGCCGGCGGTCGTTTAGCCTTTATTAAGCTAAGTAGAGGGCTTTTTCGGCGACTTACGTTAAGGATTTGTAAATTCGCCGCGCTGAATAAGTTGCCAGTGATCACCCTGTTCTCGGTTATTTggtc                                                                                                                                                                                    >  1:58092/1‑248 (MQ=255)                                                                     catACAACTAATAAACGTTGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAATAGTGGCa                                                                                                                                                                       <  1:280238/251‑1 (MQ=255)                                                                      atACAACGAATAAACGGGGTGAATCCAATTGGCGAGATTTATTTTTTATAAAAGTATCCTAAGTAAACAGAAGGAGATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCACGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTGTCTGCGACGTACGTTAAGGATTTGTAAATTCACACCGCGTAAAAAGTTGACTGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCa                                                                                                                                                                       <  2:99261/250‑1 (MQ=255)                                                                                             tCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTgg                                                                                                                                               >  1:304579/1‑251 (MQ=255)                                                                                                      cGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCAccc                                                                                                                                       <  1:276442/250‑1 (MQ=255)                                                                                                                                       aaCAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCggg                                                                                                     <  1:207581/251‑1 (MQ=255)                                                                                                                                            aaGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACa                                                                                                                                                                                                                 <  1:65300/138‑1 (MQ=255)                                                                                                                                            aaGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACa                                                                                                                                                                                                                 >  2:65300/1‑138 (MQ=255)                                                                                                                                                                         aCCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGAt                                                                   <  1:173548/251‑1 (MQ=255)                                                                                                                                                                                            aaTAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGattatt                                                                                                                                                   >  1:200040/1‑152 (MQ=255)                                                                                                                                                                                            aaTAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGattatt                                                                                                                                                   <  2:200040/152‑1 (MQ=255)                                                                                                                                                                                                         tttttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGagccagcc                                    >  1:19368/1‑250 (MQ=255)                                                                                                                                                                                                                              tttttCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTATTAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGACACACCTGGCGCCAGCCAACCGCTGGGTCGCACA‑TGGAt             >  2:206155/1‑251 (MQ=255)                                                                                                                                                                                                                                tttCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGAAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGGACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCG‑ACAATGGATCt           >  1:351740/1‑251 (MQ=255)                                                                                                                                                                                                                                         cTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACA‑TGGATCTGGTGAtatt  >  1:203577/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 TTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACA‑TGGATCTGGTGATATT  >  NC_000913/4640621‑4641103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑