Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,956	T→C	L117L (CTA→CTG)	arcA ←	DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,956	0	T	C	82.9%	68.0 / 4.8	35	L117L (CTA→CTG)	arcA	DNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base C (18/11);  total (21/14)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

CGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGC  >  NC_000913/4639719‑4640190                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           tggTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCAc                                                                                                                                                                                                                               >  1:106846/2‑251 (MQ=255)       cAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCGTTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCg                                                                                                                                                                                                                         >  1:186042/1‑251 (MQ=255)        aGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGAGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCACCCATTGAACTTGTAGCTATCACCGCTACGACGTTCTTCGCTGACGGTACCCAGATTAATAGTACGGGACAGCAGGACGCGTGCACGAGTCGt                                                                                                                                                                                                                        >  1:35772/1‑251 (MQ=255)           tCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTc                                                                                                                                                                                                                       >  1:189942/1‑249 (MQ=255)                       aTTTTCTTCAGCAGTTCAGCACGAGACTGAATGTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACTAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGtt                                                                                                                                                                                                         <  1:248693/251‑1 (MQ=255)                         tttCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCAGGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTg                                                                                                                                                                                                        <  2:301040/250‑1 (MQ=255)                           tcttcAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGATACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAAc                                                                                                                                                                                                     >  2:144556/1‑251 (MQ=255)                             ttcAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACgg                                                                                                                                                                                                   >  1:157495/1‑251 (MQ=255)                                     ttCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTg                                                                                                                                                                                            >  1:99249/1‑250 (MQ=255)                                          cACGGGACTTAAATTGGCCTGGGTTTTCACCGAAGTGAGGCATGGCGCGGAAATCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCAG‑ATCAACGAACGGCTGTTGATGTCCAGTTCACAACCATTGCAATTGTAGCTTTCAACGCTCCGACGTTCTTCGCTGACAGTAACCAGATTCATGGTCAGGGACAGTGGGTTGCGTGCCCGAGTCGTCGGTTCACGCGGGTAGAAAGGTTTGGTGATGt                                                                                                                                                                                        >  2:148735/1‑249 (MQ=255)                                                aCTGAAGTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCt                                                                                                                                                                                <  2:189942/251‑1 (MQ=255)                                                         ggCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGAtt                                                                                                                                                                       <  2:101707/250‑1 (MQ=255)                                                                        agaagagaagCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGCTGTAGTCATCTGCACCGATTCCGAGGCCGAGAAtt                                                                                                                                                        >  1:258129/1‑251 (MQ=255)                                                                                         cgGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCAAGTTCCCCACCATTGAACTTGTAGCATTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGACCGGGACCGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGCGGCCGAGAATTTTATCGACTTCGTTGTc                                                                                                                                       >  1:75435/1‑251 (MQ=255)                                                                                            aaCTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACg                                                                                                                                    >  1:91421/1‑251 (MQ=255)                                                                                                     cgcgGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTc                                                                                                                             >  1:199002/1‑249 (MQ=255)                                                                                                          cAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAAc                                                                                                                       >  1:99893/1‑250 (MQ=255)                                                                                                            gCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTGCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATc                                                                                                                    <  1:360734/251‑1 (MQ=255)                                                                                                                     gcgcGCCATCAGGGCCG‑ATCAACGAACGGCTGTTTATGTCCAGTTCCCAACCATTGAACTTGTAGATTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATGCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATGTCGAGGCCGAGAATTTTATCGACTTCGTTGGCACGGCCAGTCAGGAACATCAACGCaa                                                                                                             <  2:247634/246‑1 (MQ=255)                                                                                                                        cgcaaTCAGGGCCGTAT‑AACGAACGGCTGTTGATGTCAAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGGCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTc                                                                                                        <  2:99249/247‑1 (MQ=255)                                                                                                                            aTCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCt                                                                                                    >  1:244842/1‑251 (MQ=255)                                                                                                                                gtgcag‑ATCAACGAACTGCTGTTGATGGCGAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCGGGCAGTACCCAGAGTCATGGTACGGGACAGCAGGTTGCGTGCTCGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCTATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTc                                                                                                <  2:68289/246‑1 (MQ=255)                                                                                                                                                       tGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCCCGCGCTAAc                                                                             >  1:304971/1‑248 (MQ=255)                                                                                                                                                              cAGTTCCCATCCATTGATCGTTTAGCTTTCAACGCTACGACGTTTTTCGCTGACATTACCCAGATGCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCAcc                                                                                                                                                                           <  1:348528/147‑1 (MQ=255)                                                                                                                                                               aGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGtt                                                                  <  1:191426/251‑1 (MQ=255)                                                                                                                                                                    ccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGGTCTTAc                                                             <  1:151945/251‑1 (MQ=255)                                                                                                                                                                                      gCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATcc                                            >  1:2444/1‑250 (MQ=255)                                                                                                                                                                                            aaCGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCCCGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTCTTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCCAAGCAACATTCGCCAGATAGCGCAGTTCTAGCGCTAACAGAAGCCCGTTCTTCCCTGGCatagtgctatcaattcta                                      >  2:316163/1‑233 (MQ=255)                                                                                                                                                                                                      cgTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGAt                           >  1:144159/1‑251 (MQ=255)                                                                                                                                                                                                       gTTCTTCGCTGCCAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGACAGTTCACGCGGGCTGAACGGATTGGTGATGTAGTCATCTGAACCGATTTCGAGTCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCTACGAAACATTCGCCTGATCGCACAGTTCCCGCGCTAAAAGAAGACAGTTCTTACCCCGACGATTGATATACATGAttctcaggtagatg                          >  2:54293/1‑238 (MQ=255)                                                                                                                                                                                                       gTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATg                          >  1:93376/1‑251 (MQ=255)                                                                                                                                                                                                                        cccAGATGCATGGTACGGGACAGTAGGTTGCGTGTACGAATCGACAGTTCACGAGGGTTGAACGTTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATACGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGTTTGATGTCATATTCAGAGAGGAt         <  2:186042/251‑1 (MQ=255)                                                                                                                                                                                                                          cAGATTCATGGAACGGGACAGCAGGTTGAGTGCACGAATCGTCAGTTCACGCGGGTTGATCGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCAAGGCCAGTCAGGAAACTCAACGCAACATTAGCCTGATCGCGCAGTTCACGCACTACCAGAAGACCGTTCTTACCCGGCAGATTGATCTCCATGATCACCAGGTTGATGTCTTTTTCAGAAGGGATCt       >  2:207161/1‑251 (MQ=255)                                                                                                                                                                                                                             aTTCATGGTACGGGACAGCAGGTTGCGTGCACGAAGCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGAt    <  2:106846/251‑1 (MQ=255)                                                                                                                                                                                                                                cATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGc  <  1:52002/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           CGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCG‑ATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGC  >  NC_000913/4639719‑4640190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑