Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,764	G→A	S181S (TCC→TCT)	arcA ←	DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,764	0	G	A	80.8%	49.9 / 2.8	28	S181S (TCC→TCT)	arcA	DNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base G (5/0);  new base A (13/8);  total (20/8)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

ATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGT  >  NC_000913/4639527‑4640008                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     aTAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCCCGGCCGGTCATTTTCTTCAGCAGTTCAGCACGCGACTGAATTTTGc                                                                                                                                                                                                                                         >  1:68289/1‑251 (MQ=255)   aaGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCt                                                                                                                                                                                                                                       >  1:301040/1‑251 (MQ=255)     ggTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTgg                                                                                                                                                                                                                                     <  1:146153/251‑1 (MQ=255)                                      gTTTTTTTTGACGGGGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGTTCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTTCCTGGGTTTTCACAGAAGGGAAGAATGGAACGGAACTc                                                                                                                                                                                                    >  1:333898/1‑251 (MQ=255)                                                   gtggtAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGGGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGAATGAATTTTGCCTGGGTTTTCACAGTAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGc                                                                                                                                                                                       >  1:357967/1‑251 (MQ=255)                                                     ggtAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCCCGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGGCTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCtt                                                                                                                                                                                     >  1:224012/1‑251 (MQ=255)                                                        aaaGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTa                                                                                                                                                                                  <  1:98443/251‑1 (MQ=255)                                                                     cTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTAGTGTGGTTTCAGCTCCCGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGGCTGAATTTTGCCTGGGTTTTCACCGACGTGAAGCAAGGCGCGGAACTCGCTGCGCGGCAGCATGTACTGCTCGCCATc                                                                                                                                                                      >  1:57159/1‑250 (MQ=255)                                                                                     gAAGCGATAACCTGTGCCGGGGATGGGGGCTATGATTTCCGGCGTATCCGGCTTAGATTCGAAAAGTTTACGAATCCGGCGTATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGCCTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGTCGCGGAACTCGCGGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCaacgaac                                                                                                                                                     <  2:308999/251‑1 (MQ=255)                                                                                                   cACCGTGTATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTc                                                                                                                                        <  1:339697/250‑1 (MQ=255)                                                                                                                     tgatTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAAc                                                                                                                     >  1:118283/1‑251 (MQ=255)                                                                                                                            cggcgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGGCACGTCTACAGTACGGTCGTGTGGTCTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGGTGTCCAGTTCCCATCCATTGAACTTGTAGc                                                                                                              <  1:114367/251‑1 (MQ=255)                                                                                                                                                         tACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGCAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGAc                                                                                  >  1:247634/1‑250 (MQ=255)                                                                                                                                                                 cggcgaATCGTCACGTCTACAGTACGGTCGTGAGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGCGACTGAATTTTGCCTGGGTTTTCACAGAAGTGCAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGCACTTGTCGCTTTAAACGCTACGGCGTTCTTCGCTGACAGTAccc                                                                           >  2:19597/1‑249 (MQ=255)                                                                                                                                                                  ggcggATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCCGTTCCCAACCATTGAACTTGTAGCTTTCAACGCAACGACGTTCTTCGCTGACAGTAccc                                                                           >  1:238159/1‑248 (MQ=255)                                                                                                                                                                      gATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTAACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAATGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGACCGATCAACGAACGGCCGTTGATGTCCAGTTACCAACCATTGAACATGTAGCTTACAACGCTACGCCGTTCTTCGCTGACAGtcacaagatta                                                                     >  2:151945/1‑240 (MQ=255)                                                                                                                                                                                                 tggTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCAc                                         >  1:106846/2‑251 (MQ=255)                                                                                                                                                                                                       cAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCGTTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCg                                   >  1:186042/1‑251 (MQ=255)                                                                                                                                                                                                        aGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGAGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCACCCATTGAACTTGTAGCTATCACCGCTACGACGTTCTTCGCTGACGGTACCCAGATTAATAGTACGGGACAGCAGGACGCGTGCACGAGTCGt                                  >  1:35772/1‑251 (MQ=255)                                                                                                                                                                                                           tCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTc                                 >  1:189942/1‑249 (MQ=255)                                                                                                                                                                                                                       aTTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATg                                                                                                                                          >  1:201178/1‑132 (MQ=255)                                                                                                                                                                                                                       aTTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATg                                                                                                                                          <  2:201178/132‑1 (MQ=255)                                                                                                                                                                                                                       aTTTTCTTCAGCAGTTCAGCACGAGACTGAATGTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACTAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGtt                   <  1:248693/251‑1 (MQ=255)                                                                                                                                                                                                                         tttCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCAGGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTg                  <  2:301040/250‑1 (MQ=255)                                                                                                                                                                                                                           tcttcAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGATACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAAc               >  2:144556/1‑251 (MQ=255)                                                                                                                                                                                                                             ttcAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGCAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACgg             >  1:157495/1‑251 (MQ=255)                                                                                                                                                                                                                                     ttCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTg      >  1:99249/1‑250 (MQ=255)                                                                                                                                                                                                                                          cACGGGACTTAAATTGGCCTGGGTTTTCACCGAAGTGAGGCATGGCGCGGAAATCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCAGATCAACGAACGGCTGTTGATGTCCAGTTCACAACCATTGCAATTGTAGCTTTCAACGCTCCGACGTTCTTCGCTGACAGTAACCAGATTCATGGTCAGGGACAGTGGGTTGCGTGCCCGAGTCGTCGGTTCACGCGGGTAGAAAGGTTTGGTGATGt  >  2:148735/1‑249 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     ATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGT  >  NC_000913/4639527‑4640008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑