Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,608	G→A	C233C (TGC→TGT)	arcA ←	DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,608	0	G	A	94.4%	42.6 / ‑3.8	18	C233C (TGC→TGT)	arcA	DNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (9/8);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

CAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATC  >  NC_000913/4639365‑4639844                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             aaaCTCGGCCGATAGCGGGCTGTTGTTGGGTACCAGTGTGCTGGTGGTGGCGCTGGCCGGCAGGATGTTTGTGACTCTTAAAATCGACTGGTATGCTTTTTCCCTCCCGAAAATGAAAGCAAGTAAAAAAGTTACAATGGCCGATCAATTACGTTTCTGGAAATAAGTTTGAAAAATAAAAACGGCGTTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGa                                                                                                                                                                                                                                          <  1:129224/247‑1 (MQ=255)        gCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACGCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATaa                                                                                                                                                                                                                                  <  1:299231/249‑1 (MQ=255)             tAGCGCGCTGTTGTTGGGAACCAGTGAGCTGGTGGTTGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGCTCAATTACGTATCTGGAAATAAGGTTGAAAATTAAAAACGGCGCTAAAAAGAGCCGTTTTTTTTGACGGTGGAAAAGCCGAATAATCTTCCACATCACAACAGAAGCGATAACATTCAc                                                                                                                                                                                                                           >  2:191426/1‑251 (MQ=255)                       gttatgGGAACCAGTGTGATGGTGGTGGGGCTGTCCGGCATGATGTTTGTGACTCGTAAATTCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGAtggt                                                                                                                                                                                                                 <  2:89709/247‑1 (MQ=255)                             ggAACCAGTGTGCTGGAGGTGGCGCCGGCCGGCATGATGTTTGGGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTAAAATGGACGATCAATTACGAATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAACAAGCGCCGTTTTTTTTGTCGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAAACTTCACCGTGGATGGGGGCgat                                                                                                                                                                                                           >  1:308714/1‑251 (MQ=255)                                  cAGTGTGCGTGTGGTGGCGCTGGCCGGCATGATGTTTTTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGAttt                                                                                                                                                                                                      <  1:356645/251‑1 (MQ=255)                                                                             cGTAATATCGACTGGGATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGAGCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGACGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcggcg                                                                                                                                                           <  1:172394/251‑1 (MQ=255)                                                                                                                               agaagTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAATCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACATAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGTCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCTCGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCa                                                                                                         <  1:369281/251‑1 (MQ=255)                                                                                                                                      aCAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTttctt                                                                                                  >  1:101707/1‑251 (MQ=255)                                                                                                                                            gACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTcagcag                                                                                            <  1:101945/251‑1 (MQ=255)                                                                                                                                                                   aTAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCCCGGCCGGTCATTTTCTTCAGCAGTTCAGCACGCGACTGAATTTTGc                                                                     >  1:68289/1‑251 (MQ=255)                                                                                                                                                                     aaGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCt                                                                   >  1:301040/1‑251 (MQ=255)                                                                                                                                                                       ggTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTgg                                                                 <  1:146153/251‑1 (MQ=255)                                                                                                                                                                                                        gTTTTTTTTGACGGGGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGTTCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTTCCTGGGTTTTCACAGAAGGGAAGAATGGAACGGAACTc                                >  1:333898/1‑251 (MQ=255)                                                                                                                                                                                                                     gtggtAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGGGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGAATGAATTTTGCCTGGGTTTTCACAGTAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGc                   >  1:357967/1‑251 (MQ=255)                                                                                                                                                                                                                       ggtAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCCCGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGGCTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCtt                 >  1:224012/1‑251 (MQ=255)                                                                                                                                                                                                                          aaaGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTa              <  1:98443/251‑1 (MQ=255)                                                                                                                                                                                                                                       cTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTAGTGTGGTTTCAGCTCCCGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGGCTGAATTTTGCCTGGGTTTTCACCGACGTGAAGCAAGGCGCGGAACTCGCTGCGCGGCAGCATGTACTGCTCGCCATc  >  1:57159/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             CAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATC  >  NC_000913/4639365‑4639844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑