Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,005	G→C	P276P (CCG→CCC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,005	0	G	C	90.0%	74.3 / 3.4	30	P276P (CCG→CCC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base C (11/16);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

GCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTG‑GCGCTTTCTGAACATACCGGTTTTACCGTGGCATG  >  NC_000913/4638797‑4639235                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        gCGATATTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCa                                                                                                                                                                                                >  1:128722/1‑250 (MQ=255)        ttCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTaa                                                                                                                                                                                           <  1:126504/248‑1 (MQ=255)                            gTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTGTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGcc                                                                                                                                                                    <  1:153869/251‑1 (MQ=255)                               aTAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCAtt                                                                                                                                                                 >  1:217844/1‑251 (MQ=255)                                               aaCCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAAcg                                                                                                                                                                                         >  1:58947/1‑210 (MQ=255)                                                       aCTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCTTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATg                                                                                                                                         <  1:211446/251‑1 (MQ=255)                                                          ggCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCg                                                                                                                                      <  1:221287/251‑1 (MQ=255)                                                                        tttttAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTg                                                                                                                         <  1:351234/250‑1 (MQ=255)                                                                           tgaGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGaaa                                                                                                                      <  1:205781/248‑1 (MQ=255)                                                                                 gATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGGGTCGCCGTGACGACGCCACCCGATCAATACCAATTAACGGGCCGGGGGCCTACGGCCGCCATTCTGCTGATTGCACAGACTTTTATGGCGTTCTTTGTTTTTTACACGCTCAc                                                                                                               >  1:105185/1‑251 (MQ=255)                                                                                  gttttttgccacgcAAACGGGAAGGTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCGGGCAATGATACCGGCTGGAAAAACTTCCCCGCGTTTAGCGTCGCAGTGATGACGCCAGTCGATCAATACCAATTAACGGTCCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCAcc                                                                                                              <  2:109061/238‑1 (MQ=255)                                                                                  attttttgccAGCCAAACGGGAATTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGCTACCGGCTGGGAAAAATTCCCCGCGTTTAGCGTCGACGTGACGCCGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTAATCCATTCTGCTGATTGCACTCACTTTTACGGCGTTCTTTGTTTTTGAAACGCTCAcc                                                                                                              >  2:57378/9‑251 (MQ=255)                                                                                                aaaCGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTaca                                                                                                <  1:309966/251‑1 (MQ=255)                                                                                                   cGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGCCTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACAccc                                                                                             >  1:376688/1‑251 (MQ=255)                                                                                                    gggAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTa                                                                                                                                                                                            <  2:39171/155‑1 (MQ=255)                                                                                                    gggAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTa                                                                                                                                                                                            >  1:39171/1‑155 (MQ=255)                                                                                                        aGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATCAACTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGACAACTTCCCCGCGTTTAGCGTCGCCGTGACGACGCCAGCCGGTCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGAGATCTTTGTTTTTGCAACGCTCACCGCGCAACGTTTACACCCAATGc                                                                                        >  1:172378/1‑251 (MQ=255)                                                                                                                aGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTg                                                                                <  1:250878/251‑1 (MQ=255)                                                                                                                              gcgcAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTGTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTc                                                                  <  1:131134/251‑1 (MQ=255)                                                                                                                                            cagcTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGAc                                                                                                                                                                              >  1:335766/1‑129 (MQ=255)                                                                                                                                            cagcTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGAGACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGAc                                                                                                                                                                              <  2:335766/129‑1 (MQ=255)                                                                                                                                                      gCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGctct                                           <  1:57378/250‑1 (MQ=255)                                                                                                                                                         aataatCTCGGTTAGCGTTTTGCTTCAGGCAATGATTCCGGCTGGGAAAACTTCCTCTCGTTTAGCGTCGCAGTGGCTACGCCAGCCGATCAATACCAATTTACGGACCGGGCGATTAAGTACGCCATTCTGCTGATTGCACTGACTTTTAGGGCTTTCTTTGTTGTTGAAACGTTCACCGCGCAACGTTTTCACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCt                                       <  1:356427/251‑1 (MQ=255)                                                                                                                                                         aataagCTCGGTGGGCGTTTTGCTTCGGGCAATGATACCGGCGGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCt                                       <  1:110793/251‑1 (MQ=255)                                                                                                                                                                ccggggatctttttGATTCAGTCAATGAAACCGGCGGGGAAAACTTCCCCGTGTTTAGGGTCGCAGTGACGGCGTCTGCCGAACAATACCAATTAACTGACCGGGCTACTAAGGACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTGTTGAAACGCTCACCGCGCAACGTTTACCCCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTG‑GCGCtt                               <  2:217844/241‑1 (MQ=255)                                                                                                                                                                    tGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGGCTAAGTACGCCATTCTTCTGATTGCACTGACTTTTAAGGCGTTCTTTGTTTTTGAACCGCTCACCGCGAAACGTTTAAACCCAATGCAATCTTTGCTGGTGTGGCTTTCTTTTGTGATGTTTTATTTGCTCTTG‑TGTGCGCTTTCTg                           >  2:130521/1‑251 (MQ=255)                                                                                                                                                                                 tCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTG‑GCGCTTTCTGAACATATCGGttt              >  1:138792/1‑251 (MQ=255)                                                                                                                                                                                        aTGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTG‑GCGCTTTCTGAACATATCGGTTTTACCGTg       >  1:175734/1‑251 (MQ=255)                                                                                                                                                                                             cccGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTG‑GCGCTTTCTGAACATATCGGTTTTACCGTGGCATg  <  1:377818/250‑1 (MQ=255)                                                                                                                                                                                             aCCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTG‑GCGCTTTCTGAACATATCGGTTTTACCGTGGCATg  <  1:82269/251‑1 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        GCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTG‑GCGCTTTCTGAACATACCGGTTTTACCGTGGCATG  >  NC_000913/4638797‑4639235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑