| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,638,282 | T→C | A35A (GCT→GCC) | creD → | putative inner membrane protein CreD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,638,282 | 0 | T | C | 93.9% | 86.8 / ‑4.1 | 34 | A35A (GCT→GCC) | creD | putative inner membrane protein CreD |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (16/15); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
GTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAG > NC_000913/4638035‑4638521 | gtttgtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGAt > 1:229827/1‑251 (MQ=255) tttgtttAACGGCGAAGTCACGCTGCGCAACGTGCCGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGAtt > 1:102508/1‑251 (MQ=255) cGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTG‑TTTGGTGCAGTATTG‑CTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCg > 1:279275/1‑251 (MQ=255) cGGCGAAGTCACGCTGCGCAACGGGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCATCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCg < 1:69904/251‑1 (MQ=255) gtgAAGTCACTCTGCGCAACGTGCAGGAAGGTGGC‑TGTCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCGTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑GTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGAt < 2:337907/249‑1 (MQ=255) gTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGcc > 1:161739/1‑250 (MQ=255) aGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGt < 1:203529/251‑1 (MQ=255) ggttgCGTG‑CTGGCCTCGCTTCGACTTCACCGTTACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAg < 1:7864/247‑1 (MQ=255) ggTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTAcc > 1:344765/1‑249 (MQ=255) gtttcaccgtctccGTCACTTCCTATAGCTTCAAATTCTTCCCAAATAGCCTGCGTATCCTGTTGCAATTTTAAAGGAGGTGACGATGTTGAAATCCCCCCTGTTCTGGAAAATGTCCA‑CCTGGTTTGGTGCAGTATTG‑CTGTTGTTTATTCCGATAATGCTGATTCTGCAAGTGATTGTCGAACGTGCCGATTACCGTAGAGATGTTGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCACAATCTCg < 2:211660/239‑1 (MQ=255) cGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTgg < 1:314782/251‑1 (MQ=255) tagccTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGc > 1:164264/5‑250 (MQ=255) ctgctgCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATaa > 1:291218/1‑250 (MQ=255) cagctgtCATTGCAAAGGAGAAGAATATGTTGACAACCCCCCTGTACTGTAATATGCCCACCTTG‑TTTGGTGTAG‑GTGGTCGTTTGTGGATTTCTATATTGCTTTTGCGGCAAGTGATTGTCTTACGTGCCGGTTACCGTAGCGATGGGGAAGTTGCGATTCACCAAAGTACCAGCGGGTCGCAAAAACGCGTTGGGCCTCGTAACTCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATaaa < 2:202727/249‑1 (MQ=255) gctgcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAAc < 1:268299/251‑1 (MQ=255) gcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGt < 1:381024/251‑1 (MQ=255) aTTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTgg > 1:228434/1‑250 (MQ=255) ttGCAAAGGAGAAGACTATGTTGAAATCCGCCCTGTGCTTGAAAATGACCACCTTG‑TATGAGGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTTGAACGTGCAGATTACCGGAGCGGTGTGGCAGATGCGATTCGCCAAAGTACCAGCGGGCCGAAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGATGTATACGGGGCAGGAAGAggtacaaaccggcagg > 2:351465/1‑237 (MQ=255) cAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCgg > 1:284779/1‑251 (MQ=255) agaCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAgg > 1:127580/1‑250 (MQ=255) gTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAGCCGTGGAGCGGAAACGAAGTTTTATcc > 1:296917/1‑251 (MQ=255) gAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCTCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAgg < 2:303665/209‑1 (MQ=255) gAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAgg > 1:303665/1‑209 (MQ=255) ccccccTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGAc < 1:148538/184‑1 (MQ=255) gtccaccttG‑TTTGGTGCAGTACTG‑CTGTGTTTGATTCCGTTAATGCTTATACGGCAAGTGATTGACGAACGTGCTGATGACCGTAGTGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGTGCCGCAAAAACTCGTTGGGTCGCTTATCGCGATTCCTGTGAACGAGCTTTATACGGTGCATGTAGAGGATATAACCGTGGATCGGAAACGTAGATTTATCCATTTTTGGTTACCGGAGTCAttgtta < 2:151729/242‑4 (MQ=255) cttG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCAttgatggttgatg > 1:378130/3‑251 (MQ=255) gattcctataagtttgtatcgtcaagTGATTGTCGAATGTGCCGATTCCCTTAGCGATGAGGCAGATGCGATCCGCCAAAGGACCAGCGGGCCGCAAAAACGCGTTGGTCCGCTTATCGCTATTCCTGAGACCGAGCTTTATACGGTGCAGGAAGAGGATAACACCGTGGAGCTGAAACGAAGTTTTATCCATTTTTGGTTACCTGATTCATTGATGGTTGATGGCAATCAGAACGTTGAAGATCGCAAg < 2:143313/225‑1 (MQ=255) gATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTggg < 2:12085/109‑1 (MQ=255) gATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTggg > 1:12085/1‑109 (MQ=255) tGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTAACGTAGCGATGTGGTAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTTCAGGGAGAGGATAAAACCGTGGAGCGGAAACGAAATTTTCTCCCTTTTTGGTTACCTGGGTCATGGTTGGTTGATGGCAATCAGAAAGTGGAAGAACGCAAGATaagggtgac > 2:351234/1‑242 (MQ=255) gATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATAcc < 1:349761/250‑1 (MQ=255) gATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCg > 1:63570/1‑251 (MQ=255) gcaagTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAgg > 1:274922/5‑251 (MQ=255) gTCGAACGTGCCGATTACCGTAGCGATGGGGAAGATTCGATTCGCTAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCTGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAAATAGGGATTTATACCGGTCAGGTCTGGCACAg < 2:161739/251‑1 (MQ=255) | GTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAG > NC_000913/4638035‑4638521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |