| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,638,212 | G→C | S12T (AGC→ACC) | creD → | putative inner membrane protein CreD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,638,212 | 0 | G | C | 97.0% | 97.0 / NA | 35 | S12T (AGC→ACC) | creD | putative inner membrane protein CreD |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (16/16); minor base A (1/0); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCC > NC_000913/4637981‑4638430 | cAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTg < 2:124769/251‑1 (MQ=255) ggtcAAAAAAGCAGCGGACTGGGGTTAGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTGACGCTGCGCCACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTAAACGTCACTTCACATAGCATCCAATTCTTCCCACATAGCCTTCGTCTCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAACATGATCACCTTG‑TTTGGTGCAGTATTT‑Ctgtt > 2:268299/4‑251 (MQ=255) ggCAAAAAAGCAGCGGTCTGGGGGTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATGCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑Ctgttg < 2:108942/251‑1 (MQ=255) gCAAAAAAGCAGCGGTCTGGGGTTGTCGTTCGTCAGCGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑Ctgttgt > 1:118317/1‑251 (MQ=255) tctgTCTGGTGTTAGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGAGTG‑CTGGCATCGCTTCGACTTAACCGTCACTTCACATAGCTTCAAATTATTCCCACATAGCCTTCGTATCATGCTGCCCTTGCAAAGGTGATGACTATGTTTAGTTCCCCCCTGTTCTGGAAAAGGACCACATTG‑TTTGGTGCAGTCTTG‑CTGATGTTGGTTCCGAt > 2:120951/4‑250 (MQ=255) gTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTACCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATg < 1:147970/251‑1 (MQ=255) tGGGGTTGGCGTTTGTCAGGGAGGTCTCCCGTTGGTGTAACGGGGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCCCATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCt < 2:10534/250‑1 (MQ=255) cGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCa > 1:291620/1‑250 (MQ=255) ggctgTCAGTGAGGTCTCTCGTTTGTGTAACGGCGAAGTCACGCTGGGCAACGTGCAGGAATGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGTCTATGTTGAAATCCCCCCGGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGGTGTTGATTCCGATAATGCTGATTCGGCaag < 2:47727/247‑3 (MQ=255) gTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTc < 1:229590/251‑1 (MQ=255) cGTTTGTTTAACGACGAAGTCACGCTGCGCAACGTGCAGGAAGGCGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGCGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCAACTTG‑TTTGTTACAGAATTT‑CTGTTGTTGATTACGTTAATGCTGTTTCGCCACAGGATTGTCTAACGTGcggt > 2:195437/1‑248 (MQ=255) gtttgtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGAt > 1:229827/1‑251 (MQ=255) tttgtttAACGGCGAAGTCACGCTGCGCAACGTGCCGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGAtt > 1:102508/1‑251 (MQ=255) cGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTG‑TTTGGTGCAGTATTG‑CTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCg > 1:279275/1‑251 (MQ=255) cGGCGAAGTCACGCTGCGCAACGGGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCATCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCg < 1:69904/251‑1 (MQ=255) gtgAAGTCACTCTGCGCAACGTGCAGGAAGGTGGC‑TGTCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCGTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑GTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGAt < 2:337907/249‑1 (MQ=255) gTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGcc > 1:161739/1‑250 (MQ=255) aGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGt < 1:203529/251‑1 (MQ=255) ggttgCGTG‑CTGGCCTCGCTTCGACTTCACCGTTACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAg < 1:7864/247‑1 (MQ=255) ggTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTAcc > 1:344765/1‑249 (MQ=255) gtttcaccgtctccGTCACTTCCTATAGCTTCAAATTCTTCCCAAATAGCCTGCGTATCCTGTTGCAATTTTAAAGGAGGTGACGATGTTGAAATCCCCCCTGTTCTGGAAAATGTCCA‑CCTGGTTTGGTGCAGTATTG‑CTGTTGTTTATTCCGATAATGCTGATTCTGCAAGTGATTGTCGAACGTGCCGATTACCGTAGAGATGTTGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCACAATCTCg < 2:211660/239‑1 (MQ=255) cGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTgg < 1:314782/251‑1 (MQ=255) tagccTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGc > 1:164264/5‑250 (MQ=255) ctgctgCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATaa > 1:291218/1‑250 (MQ=255) cagctgtCATTGCAAAGGAGAAGAATATGTTGACAACCCCCCTGTACTGTAATATGCCCACCTTG‑TTTGGTGTAG‑GTGGTCGTTTGTGGATTTCTATATTGCTTTTGCGGCAAGTGATTGTCTTACGTGCCGGTTACCGTAGCGATGGGGAAGTTGCGATTCACCAAAGTACCAGCGGGTCGCAAAAACGCGTTGGGCCTCGTAACTCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATaaa < 2:202727/249‑1 (MQ=255) gctgcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAAc < 1:268299/251‑1 (MQ=255) gcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGt < 1:381024/251‑1 (MQ=255) aTTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTgg > 1:228434/1‑250 (MQ=255) ttGCAAAGGAGAAGACTATGTTGAAATCCGCCCTGTGCTTGAAAATGACCACCTTG‑TATGAGGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTTGAACGTGCAGATTACCGGAGCGGTGTGGCAGATGCGATTCGCCAAAGTACCAGCGGGCCGAAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGATGTATACGGGGCAGGAAGAggtacaaaccggcagg > 2:351465/1‑237 (MQ=255) cAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCgg > 1:284779/1‑251 (MQ=255) agaCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAgg > 1:127580/1‑250 (MQ=255) gTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAGCCGTGGAGCGGAAACGAAGTTTTATcc > 1:296917/1‑251 (MQ=255) gAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCTCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAgg < 2:303665/209‑1 (MQ=255) gAAATCCCCCCTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAgg > 1:303665/1‑209 (MQ=255) ccccccTGTTCTGGAAAATGACCACCTTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGAc < 1:148538/184‑1 (MQ=255) | CAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTG‑CTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTG‑TTTGGTGCAGTATTG‑CTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCC > NC_000913/4637981‑4638430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |