Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,976	T→G	P427P (CCT→CCG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,976	0	T	G	100.0%	98.1 / NA	34	P427P (CCT→CCG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/19);  total (15/19)

AAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTT  >  NC_000913/4637735‑4638219                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    aaaaaaacaTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCTGTCGCATAACTCTAAGCGCCGAAGTGGATCATGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAt                                                                                                                                                                                                                                            <  1:240021/251‑1 (MQ=255)    aaaaaaTCACTCTACATGTTATGCCCACTGAGGTTAATTTTGCTGCTGAACCGTCGTTACTTGAGCAGGCTCTGTGGAATTTACTTGATAACGCCATCGATTTTACCCCCGAGAGCGTTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAATGTGCTGGATACCGGTAGTGGTATTCCTGACTACTCGCTGTCACATATTTTTGAACGCTTTTACTCTTTGCCGCATGCAAATggg                                                                                                                                                                                                                                         <  2:173717/251‑1 (MQ=255)     aaacaTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCACATGGGc                                                                                                                                                                                                                                        >  1:143313/5‑251 (MQ=255)              tctacATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAagc                                                                                                                                                                                                                                >  1:4952/5‑250 (MQ=255)                 acATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCg                                                                                                                                                                                                                            >  1:151729/2‑251 (MQ=255)                 acATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCg                                                                                                                                                                                                                            <  1:159609/250‑1 (MQ=255)                  cATGTTATTCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGGTACTGGAGCAGGCGCTGGGGAATTTACTGGAGAACGCCATCGATTTTACCCCCGAGAGCGGTCGCAGAACGCTAAGCGCCGAAGTGTATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTGTGAACGCTTTTACGCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCg                                                                                                                                                                                                                            <  2:236118/250‑1 (MQ=255)                        atgCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgg                                                                                                                                                                                                                      <  1:93393/248‑1 (MQ=255)                                             gctgctGACCCGGCGTTATTGTCGCAGGCGCTGGGGAATTTACTGGCTAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCGAAGCGCCGAAGTGGATCCGGAACACGTCGCCCTTAAGGTGTTGGATTCCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAATCTGCCGTCTGGGGTTGGCGTTCGGCAGTGAGGt                                                                                                                                                                                                <  2:276643/251‑1 (MQ=255)                                               gtcggAACCGTCGTTACTGGAGCAGGCGCTTGGGAATTTTCTGTATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATAACGCTAAGTGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCTCTGTCACGTATTTTTGAATGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTc                                                                                                                                                                                               <  2:186163/246‑1 (MQ=255)                                                gctgAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGc                                                                                                                                                                                             <  1:195276/251‑1 (MQ=255)                                                 cagAACCGGCGTTACTGGAGCAGGCGCGGGGGAATTTACTGTAGAACTCCTTCCATTTTCCCCCCTAGAGCGGGCGCAGAACGCTAAGCGACGAAGTGGACGAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCGGTCACGTATTTTTGAACGCTTGTACTCTTTGCCGCGTGCATGTGGTCAAAAAAGCATCGTTCTGGGGGTGGCGTTCGTCAGTGTGGTCgac                                                                                                                                                                                            <  2:249851/249‑3 (MQ=255)                                                   gAACCGGCGTTACGGGAGCGGGCGCTTGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCGGTCACGGATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATTGGCAAAAAAGCAGCTGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCg                                                                                                                                                                                          <  2:179516/251‑1 (MQ=255)                                                                                gaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTgcgc                                                                                                                                                              >  1:346976/2‑250 (MQ=255)                                                                                     tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTg                                                                                                                                                        >  1:10534/1‑251 (MQ=255)                                                                                               cGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTg                                                                                                                                              <  1:301558/251‑1 (MQ=255)                                                                                                          atACCACCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGACGCCCTTAAGGTGCGGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTAATTTTGAACGCTTGTACTCTTTTCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGTGTTCGTCAGTGAGGTCGGCCGTTTGTTGAACGGCGAAGTCAAGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGc                                                                                                                                    <  2:188819/249‑1 (MQ=255)                                                                                                                ccgagagcgaacgcatagcGCTAAGCGCCGAAGTGGATCAGGAACACGACGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTAACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGAAAAAAAGCGGCGGTCTGGGGTTGTCGTTCGTCAGTGGGGTCGCCCGTTTGTTTAACGTGGAAGTCACGCTGCGCATAGTGCAGGAAGGTGGCGTGCTGGctccactt                                                                                                                             >  2:69904/19‑244 (MQ=255)                                                                                                                            gCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGt                                                                                                                 >  1:108942/1‑251 (MQ=255)                                                                                                                                      aaGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACAt                                                                                                       <  1:11672/251‑1 (MQ=255)                                                                                                                                                      cAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGTTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTcc                                                                                       <  1:199855/251‑1 (MQ=255)                                                                                                                                                         gAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCATCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCcac                                                                                    >  1:20854/1‑251 (MQ=255)                                                                                                                                                         gAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTCTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAACTCTTCCcac                                                                                    <  1:374439/251‑1 (MQ=255)                                                                                                                                                               gtcgcccTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAgcc                                                                              >  1:124769/7‑249 (MQ=255)                                                                                                                                                                         aGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCct                                                                    >  1:30363/1‑251 (MQ=255)                                                                                                                                                                             gtgggttgccgttAGTGGTTTTCCTGACTTCGAGTTGTCACATATGTGTTAACGTTTTTATTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGTTCTGGGGTTGGTGTTCTGCAGTGAGGGCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACTTGCAGGAAGGTGGCTTGCTGGCCACGCTTCGACGTCACCGTCACGTCACATAGCCCCAAATTCTTCCCACATAGCCTTCGTATCctgct                                                                 <  2:274589/238‑1 (MQ=255)                                                                                                                                                                               ttgATACCGGTAGTGGTATACCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTGTGCCGCGTGCAAATGGGCAAAGAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGGTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCTCTTc                                                                                                           <  1:233226/204‑1 (MQ=255)                                                                                                                                                                               tGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGGGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGCGGTCGCCAGTTTGTTTAACGGCGGAGTCACGCTGCGCAACGAGCAGGAAGGTGGAGTGCGGGCCTCGCTTCGACTTCACCGTCACTTCACAGAGCTTCACATTCTTCACACATAGCCTTCGAATACTGCTGcc                                                              >  2:308408/1‑251 (MQ=255)                                                                                                                                                                                    atcGGTAGTGGTATTCCTGACTTCGCGCGGTCACTTATTGTTGAACGCTTTTACTTTTTGCCGCGTGCAAATGGGCAAAAATGCAGCTGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCCGGAAGGTGGCGTGCTTGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTg                                                          <  2:352194/248‑1 (MQ=255)                                                                                                                                                                                          aGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATACTGCTGCCATTGCAAAgg                                                    >  1:249687/1‑250 (MQ=255)                                                                                                                                                                                               tATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGagaagc                                              >  1:337907/1‑250 (MQ=255)                                                                                                                                                                                                             cgcTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGCAATcc                                >  1:258411/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttgtACTCTTTGCCGCGTTCAAAGGGGCAAAAAAGCAGCGGGCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGTGCAACGTGCAGGAAGGTGTCGTGCTGGCCTCGCGTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGaaaagtacca          <  2:276361/247‑7 (MQ=255)                                                                                                                                                                                                                                           ctTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGAccaccttgttt  >  1:183566/1‑241 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    AAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTT  >  NC_000913/4637735‑4638219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑