Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,943	T→G	L416L (CTT→CTG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,943	0	T	G	97.4%	109.7 / ‑3.8	39	L416L (CTT→CTG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (16/22);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

CGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCC  >  NC_000913/4637698‑4638189                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       cgccgcGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACg                                                                                                                                                                                                                                                   <  1:248712/251‑1 (MQ=255)   ctgcgTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCTACCGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCTCTGGGGAATTTACTGGATAACGCCAGCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTTACTACGCGCTGTCACg                                                                                                                                                                                                                                                   <  2:37153/247‑1 (MQ=255)           gCGAAGCGCGCACCGTGCAGTTTGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGATGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGaa                                                                                                                                                                                                                                         <  2:104248/251‑1 (MQ=255)             gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATGCCGGTAGAGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACg                                                                                                                                                                                                                                       >  1:263088/1‑251 (MQ=255)             gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACg                                                                                                                                                                                                                                       >  1:371013/1‑251 (MQ=255)                 cgcgcACCGTGCAGTTGGCAGAAAAAAAAATCAATCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCttt                                                                                                                                                                                                                                   <  1:59037/251‑1 (MQ=255)                  gcgcTCCGGGCAGTTGGCTGAAAAAAGCATCACGCGACATGTTATGCCCGCTGAGGTTAATGTGGCTGCTGACCCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCtttt                                                                                                                                                                                                                                  <  2:114885/251‑1 (MQ=255)                                      aaaaaaacaTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCTGTCGCATAACTCTAAGCGCCGAAGTGGATCATGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAt                                                                                                                                                                                                              <  1:240021/251‑1 (MQ=255)                                         aaaaaaTCACTCTACATGTTATGCCCACTGAGGTTAATTTTGCTGCTGAACCGTCGTTACTTGAGCAGGCTCTGTGGAATTTACTTGATAACGCCATCGATTTTACCCCCGAGAGCGTTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAATGTGCTGGATACCGGTAGTGGTATTCCTGACTACTCGCTGTCACATATTTTTGAACGCTTTTACTCTTTGCCGCATGCAAATggg                                                                                                                                                                                                           <  2:173717/251‑1 (MQ=255)                                          aaacaTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCACATGGGc                                                                                                                                                                                                          >  1:143313/5‑251 (MQ=255)                                                   tctacATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAagc                                                                                                                                                                                                  >  1:4952/5‑250 (MQ=255)                                                      acATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCg                                                                                                                                                                                              >  1:151729/2‑251 (MQ=255)                                                      acATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCg                                                                                                                                                                                              <  1:159609/250‑1 (MQ=255)                                                       cATGTTATTCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGGTACTGGAGCAGGCGCTGGGGAATTTACTGGAGAACGCCATCGATTTTACCCCCGAGAGCGGTCGCAGAACGCTAAGCGCCGAAGTGTATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTGTGAACGCTTTTACGCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCg                                                                                                                                                                                              <  2:236118/250‑1 (MQ=255)                                                             atgCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgg                                                                                                                                                                                        <  1:93393/248‑1 (MQ=255)                                                                                  gctgctGACCCGGCGTTATTGTCGCAGGCGCTGGGGAATTTACTGGCTAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCGAAGCGCCGAAGTGGATCCGGAACACGTCGCCCTTAAGGTGTTGGATTCCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAATCTGCCGTCTGGGGTTGGCGTTCGGCAGTGAGGt                                                                                                                                                                  <  2:276643/251‑1 (MQ=255)                                                                                    gtcggAACCGTCGTTACTGGAGCAGGCGCTTGGGAATTTTCTGTATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATAACGCTAAGTGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCTCTGTCACGTATTTTTGAATGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTc                                                                                                                                                                 <  2:186163/246‑1 (MQ=255)                                                                                     gctgAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGc                                                                                                                                                               <  1:195276/251‑1 (MQ=255)                                                                                      cagAACCGGCGTTACTGGAGCAGGCGCGGGGGAATTTACTGTAGAACTCCTTCCATTTTCCCCCCTAGAGCGGGCGCAGAACGCTAAGCGACGAAGTGGACGAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCGGTCACGTATTTTTGAACGCTTGTACTCTTTGCCGCGTGCATGTGGTCAAAAAAGCATCGTTCTGGGGGTGGCGTTCGTCAGTGTGGTCgac                                                                                                                                                              <  2:249851/249‑3 (MQ=255)                                                                                        gAACCGGCGTTACGGGAGCGGGCGCTTGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCGGTCACGGATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATTGGCAAAAAAGCAGCTGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCg                                                                                                                                                            <  2:179516/251‑1 (MQ=255)                                                                                                                     gaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTgcgc                                                                                                                                >  1:346976/2‑250 (MQ=255)                                                                                                                          tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTg                                                                                                                          >  1:10534/1‑251 (MQ=255)                                                                                                                                    cGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTg                                                                                                                <  1:301558/251‑1 (MQ=255)                                                                                                                                               atACCACCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGACGCCCTTAAGGTGCGGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTAATTTTGAACGCTTGTACTCTTTTCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGTGTTCGTCAGTGAGGTCGGCCGTTTGTTGAACGGCGAAGTCAAGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGc                                                                                                      <  2:188819/249‑1 (MQ=255)                                                                                                                                                     ccgagagcgaacgcatagcGCTAAGCGCCGAAGTGGATCAGGAACACGACGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTAACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGAAAAAAAGCGGCGGTCTGGGGTTGTCGTTCGTCAGTGGGGTCGCCCGTTTGTTTAACGTGGAAGTCACGCTGCGCATAGTGCAGGAAGGTGGCGTGCTGGctccactt                                                                                               >  2:69904/19‑244 (MQ=255)                                                                                                                                                                 gCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGt                                                                                   >  1:108942/1‑251 (MQ=255)                                                                                                                                                                           aaGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACAt                                                                         <  1:11672/251‑1 (MQ=255)                                                                                                                                                                                           cAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGTTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTcc                                                         <  1:199855/251‑1 (MQ=255)                                                                                                                                                                                              gAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCATCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCcac                                                      >  1:20854/1‑251 (MQ=255)                                                                                                                                                                                              gAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTCTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAACTCTTCCcac                                                      <  1:374439/251‑1 (MQ=255)                                                                                                                                                                                                    gtcgcccTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAgcc                                                >  1:124769/7‑249 (MQ=255)                                                                                                                                                                                                              aGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCct                                      >  1:30363/1‑251 (MQ=255)                                                                                                                                                                                                                  gtgggttgccgttAGTGGTTTTCCTGACTTCGAGTTGTCACATATGTGTTAACGTTTTTATTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGTTCTGGGGTTGGTGTTCTGCAGTGAGGGCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACTTGCAGGAAGGTGGCTTGCTGGCCACGCTTCGACGTCACCGTCACGTCACATAGCCCCAAATTCTTCCCACATAGCCTTCGTATCctgct                                   <  2:274589/238‑1 (MQ=255)                                                                                                                                                                                                                    ttgATACCGGTAGTGGTATACCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTGTGCCGCGTGCAAATGGGCAAAGAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGGTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCTCTTc                                                                             <  1:233226/204‑1 (MQ=255)                                                                                                                                                                                                                    tGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGGGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGCGGTCGCCAGTTTGTTTAACGGCGGAGTCACGCTGCGCAACGAGCAGGAAGGTGGAGTGCGGGCCTCGCTTCGACTTCACCGTCACTTCACAGAGCTTCACATTCTTCACACATAGCCTTCGAATACTGCTGcc                                >  2:308408/1‑251 (MQ=255)                                                                                                                                                                                                                         atcGGTAGTGGTATTCCTGACTTCGCGCGGTCACTTATTGTTGAACGCTTTTACTTTTTGCCGCGTGCAAATGGGCAAAAATGCAGCTGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCCGGAAGGTGGCGTGCTTGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTg                            <  2:352194/248‑1 (MQ=255)                                                                                                                                                                                                                               aGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATACTGCTGCCATTGCAAAgg                      >  1:249687/1‑250 (MQ=255)                                                                                                                                                                                                                                    tATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGagaagc                >  1:337907/1‑250 (MQ=255)                                                                                                                                                                                                                                                  cgcTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGCAATcc  >  1:258411/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCC  >  NC_000913/4637698‑4638189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑