Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,675	C→T	A327V (GCT→GTT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,675	0	C	T	92.5%	100.4 / ‑4.7	40	A327V (GCT→GTT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (15/22);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGT  >  NC_000913/4637428‑4637919                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     aGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAATCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTTGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTTCTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACtgttgt                                                                                                                                                                                                                                                   <  1:206600/251‑1 (MQ=255) aGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAAAGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACtgttgt                                                                                                                                                                                                                                                   >  1:179516/1‑251 (MQ=255)       cgcgTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCGTTAACCCATGAGCTAAATAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTGTGACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGAtgt                                                                                                                                                                                                                                             <  2:242057/251‑1 (MQ=255)           tGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCCCCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAACATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTggcg                                                                                                                                                                                                                                         >  1:249851/1‑251 (MQ=255)             aaGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTggcggg                                                                                                                                                                                                                                       >  1:352194/1‑250 (MQ=255)                  ggAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCATCGGAAGTGGTGTCGCGTTTTACCGACAACATTCTGACTCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCatta                                                                                                                                                                                                                                   <  1:39976/250‑1 (MQ=255)                     aGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattatttc                                                                                                                                                                                                                               >  1:142323/1‑249 (MQ=255)                         aaaaaCTATATTGAGCAGTATGTTTATACGTTAACCCATTAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGTGGAAACTTTACGCGAAGGTACGCCTCCGGAAGTGGTGGCTCGTTTTACCTACAACATTCTGACTCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAATTCGTTCTGACTGTTGTTGATGTGTCGGCATTATTgcgccg                                                                                                                                                                                                                           <  2:265707/251‑1 (MQ=255)                          aaaaCTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTcgccgc                                                                                                                                                                                                                          <  1:189215/251‑1 (MQ=255)                             aCTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGGGAATCATAAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTc                                                                                                                                                                                                                       >  1:377362/1‑251 (MQ=255)                                     gAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAg                                                                                                                                                                                                                <  1:247743/250‑1 (MQ=255)                                              gTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCgg                                                                                                                                                                                                      >  1:211660/1‑250 (MQ=255)                                                 tatgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATGCGTGTCGCGGCGGAAATTTTTCGCGAAGGTCCGCCACCTGAAGTGGTGGCTCGTTTTACCTACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGGTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTGGATGTGGCTGCATTATTTCGCCGCGTCAGCGaggctctatccgtgc                                                                                                                                                                                                    <  2:157051/244‑15 (MQ=255)                                                 tACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGTAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGGAGAGGGGGCGCGTTTTACTGGCAACATTCTGACGCAAAAAGCGCGTGTGCAGGCGTTGGTAGGAACCGTACTACGCCAGGCCAGACTGGGGAACCGTCAGGAAGTCGTTCTGACTGCTGATGATCTGGCAGCCTTTTTTCGCCGCGTCAGGGATGCGCGCAcccggcc                                                                                                                                                                                                   >  2:59037/1‑246 (MQ=255)                                                   tgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATAGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTTGCCGCGTCAGCGAAGCGCGCACCGTGCAGt                                                                                                                                                                                                 >  1:247325/5‑251 (MQ=255)                                                                   cTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGTCTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGGTGGCAGaaaaaaacat                                                                                                                                                                                 >  1:236118/1‑248 (MQ=255)                                                                     aaaaaGCCCACGTGCGGTGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGGCCGCCACCGGTAGTGGTGGCGCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaacat                                                                                                                                                                                 <  2:120724/249‑4 (MQ=255)                                                                        aagcccgcggggggcgATTCGGGGCGCGGCGGAAATTTTACGCGAATGTCCGCCACTGGAAGTGGTGGCTCGTTTTACCGACTACATTCCGACGCAAAAGTCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGATTTCGTTCGGACTGTTGTTGATGGGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCCCCGGTCAGTTGGCAGAAAAAAACATCActc                                                                                                                                                                            <  2:363832/239‑1 (MQ=255)                                                                        aaGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGCGGTGGCTCGTTTTACCAACAACATTCTGACGCAAAATGCGCGTCTGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTCTTTCGCCGCGTCAGCGAAACGCGCACCGTGCAGTTGGCAGAAAAAAACATCActc                                                                                                                                                                            >  1:54977/1‑251 (MQ=255)                                                                              ccTGGCGGCGATTCGTGGCGCAGCGGAAACTTTACGCGAAGGTCCGACACCGGAAGTGGTGGCTCGGTTTACCGACAACAGTCTGACGCAAAATGCGTGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGATTCGTCAGGAAGCCGTTCTGACTGTTGTTGATGTGGCGGCAGTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCTGTTGGCAGaaacatactgcacaatatagt                                                                                                                                                                      <  2:377362/250‑19 (MQ=255)                                                                                 ggcggcGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGtt                                                                                                                                                                    <  1:354095/250‑1 (MQ=255)                                                                                            gTGGAGCTGCGGAAATTTTACTCGAATGTACGCCACCGGAGTTTGTGGCTCGTTTTACCGACAACTTTCTGACGCAAAAGGCGCGTATGCAGGCATTGGTAGAAACGTTTCTACGCCAGGCAAGACTGTAGAATCGTCAGGATGTCGTTCTGACTGTTGTTGATTTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTTCATGTTTTGCCCACTGAg                                                                                                                                                        <  2:355470/251‑1 (MQ=255)                                                                                                 gcggcgGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGGTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTa                                                                                                                                                    >  1:186163/1‑250 (MQ=255)                                                                                                    gcggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTaatgt                                                                                                                                                <  1:348469/251‑4 (MQ=255)                                                                                                         aaTTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTtgctg                                                                                                                                           <  1:122151/251‑1 (MQ=255)                                                                                                                         ccgccaccgGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACt                                                                                                                           <  2:142323/251‑1 (MQ=255)                                                                                                                           gccaccgGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTgg                                                                                                                         >  1:274589/5‑251 (MQ=255)                                                                                                                                   aaGTGGTGTCTCGGTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGGTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGgcg                                                                                                                 <  1:171859/251‑1 (MQ=255)                                                                                                                                   aaGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGgcg                                                                                                                 >  1:276361/1‑251 (MQ=255)                                                                                                                                       ggtggCTCGTTTTACTGACAACATTCTGCCGCAAAATGCGCGGTTGCAGGCATTGGTAGAAACGTTACTATGCCATGCAAGACGGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGATGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACGCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTTCTTGAGCAGGCGCTgg                                                                                                             <  2:198925/251‑1 (MQ=255)                                                                                                                                              agTTTTACCGACAACATTCAGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGTAAGACCGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGTGAAGCGCTCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACCGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAAttt                                                                                                      <  1:371520/250‑1 (MQ=255)                                                                                                                                                             aTTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGTAGCAGGCGCTGGGGAATTTACTGGATAACGCCAt                                                                                       <  1:120509/251‑1 (MQ=255)                                                                                                                                                               tCTGCCGCAAAATGCGCGGATGCAGGTATTGGCTGAAACGTTCCTACGCTATGCAAGACTGGAGACTCTTCATGAAGTTGTTCTGACTGCTGCTGATGTGGAGGCATTATCCCGCCGCGTCTGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAACCACTCTGCAGGTTTCGCCCACCGAGGTTAACGCTGCTGCTGAACCTGCGTTACTGGCTCAGGCGCTGGGAAATTTAATGGATAACGTCATc                                                                                      <  2:99140/250‑1 (MQ=255)                                                                                                                                                                cTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGCCTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTTCCCGCGTCTGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTCCATGTTATGCCAACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTCCTGGTTAACCCCATCg                                                                                     >  1:22941/1‑250 (MQ=255)                                                                                                                                                                        aaaTGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTAccc                                                                            >  1:47727/1‑251 (MQ=255)                                                                                                                                                                          aTGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTAccccc                                                                          <  1:3141/251‑1 (MQ=255)                                                                                                                                                                                     cAGGCATTGGGAGCAACGTTCCTACTCCAGGCAAGCCTGTAGAATCGTCCGGAAGTCTTTCTGCCTGTTGTTGATGTGGCGGCATTAGTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTGCTTGATAACGCCATCGATTTTACCCCCGAGATCGGtc                                                                <  2:315553/250‑2 (MQ=255)                                                                                                                                                                                        gCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCAt                                                            <  1:209287/251‑1 (MQ=255)                                                                                                                                                                                              gTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCt                                                      <  1:197893/251‑1 (MQ=255)                                                                                                                                                                                                             cGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGa                                       <  1:41988/251‑1 (MQ=255)                                                                                                                                                                                                               ccAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTgggtc                                     >  1:104248/1‑248 (MQ=255)                                                                                                                                                                                                                                                  tGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGt  >  1:202727/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     AGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGT  >  NC_000913/4637428‑4637919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑